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The map label for this gene is celB [H]

Identifier: 116872304

GI number: 116872304

Start: 915792

End: 917078

Strand: Direct

Name: celB [H]

Synonym: lwe0886

Alternate gene names: 116872304

Gene position: 915792-917078 (Clockwise)

Preceding gene: 116872303

Following gene: 116872305

Centisome position: 32.54

GC content: 39.01

Gene sequence:

>1287_bases
GTGAATAAATTTATGGAGTTGCTTGGGGATAAATTAATGCCTCTTGCTGCAAAGTTAGGGGAAAACCGTTATTTAACAAC
TTTGCGAGACGCATTTATGTTAGCGTTTCCACTAACAATGTTTGGTTCAATTGCCGTGGTTTTAATGAATCTACCTTTTT
GGAGTGATGAAACAAAAGCTGTTTTGCAATTGTATTTAGGTAATGCGCAAAATGCGACGATGAGTATTATGACCGTTTTT
GTCGTTTTTGGGATTGGTTATTCTTTATCCAAGTATTATAAAGTAGAAGCTATTTATGGTGGTGCCGTAGCGCTTGCTAG
TTTCTTGATCTTAACGCCATTTTTCTTTAATAGTGCAAATGGGGAACTAGTGACAGGCGCGCTTTCTTTAGACAGACTTG
GTGCAAAAGGTATGTTTATTGGTATGATTACAGGTTTTATCGCGGGGGAATTATATCGTTTCTTCGTTCAACGGGACTGG
ACTATTAAGATGCCAGCTGGTGTGCCGCCAGCAGTTGCTAAATCTTTTGCAGCTTTAATTCCAGCTGTTTTGACCTTAAG
TATCTTTTTAGTAATTAACATAATCGTTCAATTCTTCTTTAATACTAACTTACATGATGTCGTGTACACAGTTATTCAAA
AGCCACTAGTTGGGTTAGGATCAGGTATTATTCCAACTCTGATTGCGCTTTTCTTTGTCCAAGTATTGTGGTTCTTCGGT
TTACATGGTCAAATTATTGTAAACTCGGTAATGGACCCGATTTGGAATACGTTAATGCTTGAAAATTTAGATGCTTACAA
AGCTGGTAATCCTTTGCCACATATTATTACGAAACCATTCATGGAAGTATTTACTGTTGGAATGGGCGGATCTGGGATGA
CACTCGCAGTAGTTATCGCGCTCGCTTTTCTAATGAAGAGTAAACAGAGTAAAGAAATCGGTCGTTTAGCACTTGGACCT
GGTATTTTTAACGTGAATGAGCCGGTATTATTCGGGATGCCAATTGTGTTAAATGCAACGATTTTAATTCCTTGGATACT
GGCGCCATTAATTGTTACGACGCTTAATTACTTTGTGATGGCTGCTGGAATTGTGCCAGCACCAACCGGAGTTTCTGTTC
CGTGGACTGTGCCAATTATCATTAATGGAATCTTGGCGACGAACTCATGGCTTGGCGGGGCACTTCAAGTGGTTGATTTC
TTTATTGTCTTAATTATTTGGTATCCATTCCTTAAACTTGTTGATCGTGCGAATATCGCGAGGGAATCGGAAGCTATTAC
CAAATAA

Upstream 100 bases:

>100_bases
GTCTTTTATTTACAAAAGACTAGACATTTAAAATTAAATGAGTATAATAATAAATAGAAAACGTTTTCTTGTAGAATAAA
ATACGTTTGGGGGAAGAAAA

Downstream 100 bases:

>100_bases
GTTATGTTTTTGTTTTCGATATGCTATAATTTGACTAGAAATGGCTAGACATTTTTAATTCAAGCGAAGGAGCGATCGAA
TTGGTTAAGTATGAATTGAT

Product: cellobiose-specific PTS, IIC component

Products: pyruvate; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm] [C]

Alternate protein names: Glucomannan utilization protein C; PTS system oligo-beta-mannoside-specific EIIC component [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MNKFMELLGDKLMPLAAKLGENRYLTTLRDAFMLAFPLTMFGSIAVVLMNLPFWSDETKAVLQLYLGNAQNATMSIMTVF
VVFGIGYSLSKYYKVEAIYGGAVALASFLILTPFFFNSANGELVTGALSLDRLGAKGMFIGMITGFIAGELYRFFVQRDW
TIKMPAGVPPAVAKSFAALIPAVLTLSIFLVINIIVQFFFNTNLHDVVYTVIQKPLVGLGSGIIPTLIALFFVQVLWFFG
LHGQIIVNSVMDPIWNTLMLENLDAYKAGNPLPHIITKPFMEVFTVGMGGSGMTLAVVIALAFLMKSKQSKEIGRLALGP
GIFNVNEPVLFGMPIVLNATILIPWILAPLIVTTLNYFVMAAGIVPAPTGVSVPWTVPIIINGILATNSWLGGALQVVDF
FIVLIIWYPFLKLVDRANIARESEAITK

Sequences:

>Translated_428_residues
MNKFMELLGDKLMPLAAKLGENRYLTTLRDAFMLAFPLTMFGSIAVVLMNLPFWSDETKAVLQLYLGNAQNATMSIMTVF
VVFGIGYSLSKYYKVEAIYGGAVALASFLILTPFFFNSANGELVTGALSLDRLGAKGMFIGMITGFIAGELYRFFVQRDW
TIKMPAGVPPAVAKSFAALIPAVLTLSIFLVINIIVQFFFNTNLHDVVYTVIQKPLVGLGSGIIPTLIALFFVQVLWFFG
LHGQIIVNSVMDPIWNTLMLENLDAYKAGNPLPHIITKPFMEVFTVGMGGSGMTLAVVIALAFLMKSKQSKEIGRLALGP
GIFNVNEPVLFGMPIVLNATILIPWILAPLIVTTLNYFVMAAGIVPAPTGVSVPWTVPIIINGILATNSWLGGALQVVDF
FIVLIIWYPFLKLVDRANIARESEAITK
>Mature_428_residues
MNKFMELLGDKLMPLAAKLGENRYLTTLRDAFMLAFPLTMFGSIAVVLMNLPFWSDETKAVLQLYLGNAQNATMSIMTVF
VVFGIGYSLSKYYKVEAIYGGAVALASFLILTPFFFNSANGELVTGALSLDRLGAKGMFIGMITGFIAGELYRFFVQRDW
TIKMPAGVPPAVAKSFAALIPAVLTLSIFLVINIIVQFFFNTNLHDVVYTVIQKPLVGLGSGIIPTLIALFFVQVLWFFG
LHGQIIVNSVMDPIWNTLMLENLDAYKAGNPLPHIITKPFMEVFTVGMGGSGMTLAVVIALAFLMKSKQSKEIGRLALGP
GIFNVNEPVLFGMPIVLNATILIPWILAPLIVTTLNYFVMAAGIVPAPTGVSVPWTVPIIINGILATNSWLGGALQVVDF
FIVLIIWYPFLKLVDRANIARESEAITK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1455

COG function: function code G; Phosphotransferase system cellobiose-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=451, Percent_Identity=30.1552106430155, Blast_Score=207, Evalue=9e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003352
- InterPro:   IPR004796
- InterPro:   IPR004501 [H]

Pfam domain/function: PF02378 PTS_EIIC [H]

EC number: NA

Molecular weight: Translated: 46766; Mature: 46766

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKFMELLGDKLMPLAAKLGENRYLTTLRDAFMLAFPLTMFGSIAVVLMNLPFWSDETKA
CCHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
VLQLYLGNAQNATMSIMTVFVVFGIGYSLSKYYKVEAIYGGAVALASFLILTPFFFNSAN
HHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHEEEEEECCCHHHHHHHHHHHHHHHCCCC
GELVTGALSLDRLGAKGMFIGMITGFIAGELYRFFVQRDWTIKMPAGVPPAVAKSFAALI
CCEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHH
PAVLTLSIFLVINIIVQFFFNTNLHDVVYTVIQKPLVGLGSGIIPTLIALFFVQVLWFFG
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
LHGQIIVNSVMDPIWNTLMLENLDAYKAGNPLPHIITKPFMEVFTVGMGGSGMTLAVVIA
CCCHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHH
LAFLMKSKQSKEIGRLALGPGIFNVNEPVLFGMPIVLNATILIPWILAPLIVTTLNYFVM
HHHHHHCCCHHHHCCCEECCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAGIVPAPTGVSVPWTVPIIINGILATNSWLGGALQVVDFFIVLIIWYPFLKLVDRANIA
HHCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RESEAITK
HHHHHCCC
>Mature Secondary Structure
MNKFMELLGDKLMPLAAKLGENRYLTTLRDAFMLAFPLTMFGSIAVVLMNLPFWSDETKA
CCHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
VLQLYLGNAQNATMSIMTVFVVFGIGYSLSKYYKVEAIYGGAVALASFLILTPFFFNSAN
HHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHEEEEEECCCHHHHHHHHHHHHHHHCCCC
GELVTGALSLDRLGAKGMFIGMITGFIAGELYRFFVQRDWTIKMPAGVPPAVAKSFAALI
CCEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHH
PAVLTLSIFLVINIIVQFFFNTNLHDVVYTVIQKPLVGLGSGIIPTLIALFFVQVLWFFG
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
LHGQIIVNSVMDPIWNTLMLENLDAYKAGNPLPHIITKPFMEVFTVGMGGSGMTLAVVIA
CCCHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHH
LAFLMKSKQSKEIGRLALGPGIFNVNEPVLFGMPIVLNATILIPWILAPLIVTTLNYFVM
HHHHHHCCCHHHHCCCEECCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAGIVPAPTGVSVPWTVPIIINGILATNSWLGGALQVVDFFIVLIIWYPFLKLVDRANIA
HHCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RESEAITK
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: diacetylchitobiose [Periplasm]; phosphoenolpyruvate; cellobiose [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + diacetylchitobiose [Periplasm] = pyruvate + diacetylchitobiose-6-phosphate [Cytoplasm] phosphoenolpyruvate + cellobiose [Periplasm] = cellobiose-6-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]