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The map label for this gene is ddl [H]

Identifier: 116872268

GI number: 116872268

Start: 880310

End: 881422

Strand: Direct

Name: ddl [H]

Synonym: lwe0848

Alternate gene names: 116872268

Gene position: 880310-881422 (Clockwise)

Preceding gene: 116872267

Following gene: 116872269

Centisome position: 31.28

GC content: 37.65

Gene sequence:

>1113_bases
ATGAAAACCAAATTAATTTTATTATACGGTGGAAAGTCTGCTGAGCACGAGGTTTCCTTACAAACAGCATTTTCAGTTAT
CAATGCGTTAGATTTGGAAAAATTTGAAGCTGAGCCAATATATATTACAAATGAAGGAGAATGGGTTCAAGGACCACTTC
TCACTGGTAAATTAGATTTTGTTGAACAATTACGTTTTTCAGCAACAAATACAGTGAAACTTGCTGCTACGGAATCAGAA
AAATCAGAAGGCGAGGCAATTAGCCCAGCTGTTTTAGAGGCAGATGATCAAGAAACGGTTGTTTTCCCACTTTTACATGG
TCCAAACGGAGAAGATGGTACCGTTCAAGGATTATTTGAAGTATTAAATATTCCGTATGTTGGTAATGGGGTTTTAGCTT
CTTCTGCTGCAATGGACAAAATCGTGATGAAGAAGATTTTTGCAGATGCTGGCATTCCGCAAGTTCCAGCAGTTGCAGTA
CGTTTAATTGATTGGAAAAATTATCAAGAAGAAATGGTTTCTGAAATGGAAGAAGTACTTACATATCCAGTCTTCGTCAA
GCCAGCAAATCTTGGTTCTAGTGTTGGTATTAGTAAAGCAACAAACAAAAAAGAATTAGAAGATGCAATGACAGAAGCAT
TTTTATATGACCGCCGTGTTGTTGTTGAACAAGGGGTGGTTGCACGTGAAATCGAAATGGGTGTACTTGGAAACGATACA
CCAGTTTGCTCGGTTCCAGGTGAAATTTTGCCAGAAGGTGCAGTGGCTACATTCTACGATTACAAAGCAAAATATCAAGA
TAATAATACTGCATTAATTATTCCGACTGAAGTGGATCCAGAAATCTTGGAACAAATGAAAGAATATGCGGTCCAAGCGT
TTTTAGGCCTTGATGCAAGTGGATTAGTACGGGCAGACTTTTTCTTAACAGATGATAATCAGCTTTTCTTAAATGAAGTC
AATACGATGCCTGGTTTTACGCCTTACAGCATGTATCCACTTCTTTGGCAGGAAACTGGTTTACCATACAGCGCGTTAAT
TGAAAGATTAGTTGATTTAGCAAAAGAACGACATGCCGCAAAAAATGCACTTAAATATAAATTAGAAGACTAA

Upstream 100 bases:

>100_bases
TTTAAAAAAGAAGATTTTTTCAACCTATTTTTTAGTTATGTCTTTTCATCTTGCTAAAACATGGTAAAATAGATAAAGCG
AATAATGACGGAGGGGTATT

Downstream 100 bases:

>100_bases
AATAAACAAGAAAGCAGTTTTCATATGCGGAAGCTGCTTTTTTATGGAAGAGAGCGATTTGATTTGAAGAAAACAGTAGG
CGAAGTAGTAGCAATGTTAG

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MKTKLILLYGGKSAEHEVSLQTAFSVINALDLEKFEAEPIYITNEGEWVQGPLLTGKLDFVEQLRFSATNTVKLAATESE
KSEGEAISPAVLEADDQETVVFPLLHGPNGEDGTVQGLFEVLNIPYVGNGVLASSAAMDKIVMKKIFADAGIPQVPAVAV
RLIDWKNYQEEMVSEMEEVLTYPVFVKPANLGSSVGISKATNKKELEDAMTEAFLYDRRVVVEQGVVAREIEMGVLGNDT
PVCSVPGEILPEGAVATFYDYKAKYQDNNTALIIPTEVDPEILEQMKEYAVQAFLGLDASGLVRADFFLTDDNQLFLNEV
NTMPGFTPYSMYPLLWQETGLPYSALIERLVDLAKERHAAKNALKYKLED

Sequences:

>Translated_370_residues
MKTKLILLYGGKSAEHEVSLQTAFSVINALDLEKFEAEPIYITNEGEWVQGPLLTGKLDFVEQLRFSATNTVKLAATESE
KSEGEAISPAVLEADDQETVVFPLLHGPNGEDGTVQGLFEVLNIPYVGNGVLASSAAMDKIVMKKIFADAGIPQVPAVAV
RLIDWKNYQEEMVSEMEEVLTYPVFVKPANLGSSVGISKATNKKELEDAMTEAFLYDRRVVVEQGVVAREIEMGVLGNDT
PVCSVPGEILPEGAVATFYDYKAKYQDNNTALIIPTEVDPEILEQMKEYAVQAFLGLDASGLVRADFFLTDDNQLFLNEV
NTMPGFTPYSMYPLLWQETGLPYSALIERLVDLAKERHAAKNALKYKLED
>Mature_370_residues
MKTKLILLYGGKSAEHEVSLQTAFSVINALDLEKFEAEPIYITNEGEWVQGPLLTGKLDFVEQLRFSATNTVKLAATESE
KSEGEAISPAVLEADDQETVVFPLLHGPNGEDGTVQGLFEVLNIPYVGNGVLASSAAMDKIVMKKIFADAGIPQVPAVAV
RLIDWKNYQEEMVSEMEEVLTYPVFVKPANLGSSVGISKATNKKELEDAMTEAFLYDRRVVVEQGVVAREIEMGVLGNDT
PVCSVPGEILPEGAVATFYDYKAKYQDNNTALIIPTEVDPEILEQMKEYAVQAFLGLDASGLVRADFFLTDDNQLFLNEV
NTMPGFTPYSMYPLLWQETGLPYSALIERLVDLAKERHAAKNALKYKLED

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=372, Percent_Identity=42.4731182795699, Blast_Score=289, Evalue=2e-79,
Organism=Escherichia coli, GI1786280, Length=358, Percent_Identity=31.5642458100559, Blast_Score=171, Evalue=6e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 40789; Mature: 40789

Theoretical pI: Translated: 4.16; Mature: 4.16

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKLILLYGGKSAEHEVSLQTAFSVINALDLEKFEAEPIYITNEGEWVQGPLLTGKLDF
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEECCEEECHHHH
VEQLRFSATNTVKLAATESEKSEGEAISPAVLEADDQETVVFPLLHGPNGEDGTVQGLFE
HHHHHHCCCCEEEEEEECCCCCCCCCCCCCEECCCCCCEEEEEEEECCCCCCCHHHHHHH
VLNIPYVGNGVLASSAAMDKIVMKKIFADAGIPQVPAVAVRLIDWKNYQEEMVSEMEEVL
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEECCCCHHHHHHHHHHHHH
TYPVFVKPANLGSSVGISKATNKKELEDAMTEAFLYDRRVVVEQGVVAREIEMGVLGNDT
CCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHCCCEEEEEEECCCCCCC
PVCSVPGEILPEGAVATFYDYKAKYQDNNTALIIPTEVDPEILEQMKEYAVQAFLGLDAS
CEEECCHHHCCCCCEEEEEECEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCC
GLVRADFFLTDDNQLFLNEVNTMPGFTPYSMYPLLWQETGLPYSALIERLVDLAKERHAA
CCEEEEEEEECCCEEEEEHHCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHH
KNALKYKLED
HHHHEEECCC
>Mature Secondary Structure
MKTKLILLYGGKSAEHEVSLQTAFSVINALDLEKFEAEPIYITNEGEWVQGPLLTGKLDF
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEECCEEECHHHH
VEQLRFSATNTVKLAATESEKSEGEAISPAVLEADDQETVVFPLLHGPNGEDGTVQGLFE
HHHHHHCCCCEEEEEEECCCCCCCCCCCCCEECCCCCCEEEEEEEECCCCCCCHHHHHHH
VLNIPYVGNGVLASSAAMDKIVMKKIFADAGIPQVPAVAVRLIDWKNYQEEMVSEMEEVL
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEECCCCHHHHHHHHHHHHH
TYPVFVKPANLGSSVGISKATNKKELEDAMTEAFLYDRRVVVEQGVVAREIEMGVLGNDT
CCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHCCCEEEEEEECCCCCCC
PVCSVPGEILPEGAVATFYDYKAKYQDNNTALIIPTEVDPEILEQMKEYAVQAFLGLDAS
CEEECCHHHCCCCCEEEEEECEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCC
GLVRADFFLTDDNQLFLNEVNTMPGFTPYSMYPLLWQETGLPYSALIERLVDLAKERHAA
CCEEEEEEEECCCEEEEEHHCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHH
KNALKYKLED
HHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]