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The map label for this gene is ispG [H]

Identifier: 116689833

GI number: 116689833

Start: 2013652

End: 2014968

Strand: Reverse

Name: ispG [H]

Synonym: Bcen2424_1812

Alternate gene names: 116689833

Gene position: 2014968-2013652 (Counterclockwise)

Preceding gene: 116689834

Following gene: 116689832

Centisome position: 57.84

GC content: 67.27

Gene sequence:

>1317_bases
ATGGATCTATCGATGCAATCCGAAGCTCAATCCCCACGCAGCAGTCAGATTTGTTCAACCGAGCCGGTGTTCGGCGGGCA
TCAGCCGCGCCGCGTATCGCATGCGGTGGATGTCCGCTGGGGCGGGCAACTCGTGACGATCGGCGGCGACGCGCCGGTGC
GCGTGCAGTCGATGACGAATACCGACACGGCCGATGCGATCGGCACGGCGATCCAGATCAAGGAACTCGCGAATGCGGGC
TCCGAGCTGGTGCGCATCACGGTCAACACGCCCGAAGCCGCGGTCGCCGTGCCGGCGATCCGCGAGCAGCTCGACCGGAT
GGGCGTGACCGTGCCGCTCGTCGGCGATTTCCACTACAACGGCCACCTGCTGCTGCGCGACTATCCGGGCTGCGCCGAGT
CGCTGTCGAAGTACCGGATCAACCCCGGTAACGTCGGTCAGGGTGCGAAGCGCGACACGCAGTTCGCGCAGATGATCGAG
GCCGCGGCGAAGTACGACAAGCCGGTGCGGATCGGCGTGAACTGGGGCAGCCTCGACCAGGACCTGCTCGCACGCATGAT
GGACGAGAACGGCGCGCGCGCGCAGCCGTGGGACGCGCAGAGCGTGATGTACGAGGCGCTGATCCAGTCGGCCATCGGCT
CGGCCGAACGCGCGGTCGAGCTCGGCCTCGGCCGTGACCGCATCGTGTTGTCGTGCAAGGTCAGCGGCGTGCAGGACCTG
ATCGCCGTGTACCGCGAACTCGGCCGCCGCTGCGGCTTCGCGCTGCACCTCGGGCTGACCGAGGCCGGCATGGGTTCGAA
GGGCATCGTCGCGTCGACGGCGGCGCTCGGCGTGTTGCTGCAGGAAGGGATCGGCGACACGATCCGCATCTCGCTGACGC
CGGAGCCGGGCGCGTCGCGCACCGGCGAGGTGATCGTCGGCCAGGAAATCCTGCAGACGATGGGCCTGCGCTCGTTCGCG
CCGATGGTGATCGCGTGCCCGGGCTGCGGCCGCACGACGAGCACGCTGTTCCAGGAGCTCGCGATGCAGATCCAGACCTA
CCTGCGCGAGCAGATGCCGGTCTGGCGCAAGGAATATCCGGGCGTCGAGAAGATGAACGTCGCGGTGATGGGCTGCATCG
TCAATGGCCCGGGCGAATCGAAGCACGCGAACATCGGCATCAGCCTGCCGGGTTCGGGCGAGAACCCGGCCGCACCCGTC
TTCATCGACGGCGAGAAGGTCAAGACGCTGCGCGGCGAGCGGATCGCCGAGGAATTCCAGCAGATCGTCAGCGACTACGT
CGCACGCAATTACGGCCGCACCGAGGCCCTGAACTAA

Upstream 100 bases:

>100_bases
CGTCAAGCGCCGTGGCGAAGGCCTGTCGTCGATCTCGGCAGGCCGTGAGGGCACGGCGCTTTTTCAATTCATGCGCCACG
TTGCGGCGCATTCCGCGTAA

Downstream 100 bases:

>100_bases
TTCGTCCACACACACGAACGATGACTGAACAGAAACGCAAGATCGAGAAGCTCACCGGCGTCAAAGGCATGAACGACATC
CTTCCGCAGGATGCCGGCCT

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MDLSMQSEAQSPRSSQICSTEPVFGGHQPRRVSHAVDVRWGGQLVTIGGDAPVRVQSMTNTDTADAIGTAIQIKELANAG
SELVRITVNTPEAAVAVPAIREQLDRMGVTVPLVGDFHYNGHLLLRDYPGCAESLSKYRINPGNVGQGAKRDTQFAQMIE
AAAKYDKPVRIGVNWGSLDQDLLARMMDENGARAQPWDAQSVMYEALIQSAIGSAERAVELGLGRDRIVLSCKVSGVQDL
IAVYRELGRRCGFALHLGLTEAGMGSKGIVASTAALGVLLQEGIGDTIRISLTPEPGASRTGEVIVGQEILQTMGLRSFA
PMVIACPGCGRTTSTLFQELAMQIQTYLREQMPVWRKEYPGVEKMNVAVMGCIVNGPGESKHANIGISLPGSGENPAAPV
FIDGEKVKTLRGERIAEEFQQIVSDYVARNYGRTEALN

Sequences:

>Translated_438_residues
MDLSMQSEAQSPRSSQICSTEPVFGGHQPRRVSHAVDVRWGGQLVTIGGDAPVRVQSMTNTDTADAIGTAIQIKELANAG
SELVRITVNTPEAAVAVPAIREQLDRMGVTVPLVGDFHYNGHLLLRDYPGCAESLSKYRINPGNVGQGAKRDTQFAQMIE
AAAKYDKPVRIGVNWGSLDQDLLARMMDENGARAQPWDAQSVMYEALIQSAIGSAERAVELGLGRDRIVLSCKVSGVQDL
IAVYRELGRRCGFALHLGLTEAGMGSKGIVASTAALGVLLQEGIGDTIRISLTPEPGASRTGEVIVGQEILQTMGLRSFA
PMVIACPGCGRTTSTLFQELAMQIQTYLREQMPVWRKEYPGVEKMNVAVMGCIVNGPGESKHANIGISLPGSGENPAAPV
FIDGEKVKTLRGERIAEEFQQIVSDYVARNYGRTEALN
>Mature_438_residues
MDLSMQSEAQSPRSSQICSTEPVFGGHQPRRVSHAVDVRWGGQLVTIGGDAPVRVQSMTNTDTADAIGTAIQIKELANAG
SELVRITVNTPEAAVAVPAIREQLDRMGVTVPLVGDFHYNGHLLLRDYPGCAESLSKYRINPGNVGQGAKRDTQFAQMIE
AAAKYDKPVRIGVNWGSLDQDLLARMMDENGARAQPWDAQSVMYEALIQSAIGSAERAVELGLGRDRIVLSCKVSGVQDL
IAVYRELGRRCGFALHLGLTEAGMGSKGIVASTAALGVLLQEGIGDTIRISLTPEPGASRTGEVIVGQEILQTMGLRSFA
PMVIACPGCGRTTSTLFQELAMQIQTYLREQMPVWRKEYPGVEKMNVAVMGCIVNGPGESKHANIGISLPGSGENPAAPV
FIDGEKVKTLRGERIAEEFQQIVSDYVARNYGRTEALN

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=376, Percent_Identity=34.5744680851064, Blast_Score=181, Evalue=6e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 47077; Mature: 47077

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLSMQSEAQSPRSSQICSTEPVFGGHQPRRVSHAVDVRWGGQLVTIGGDAPVRVQSMTN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEEEEECCEEEEECCCCCEEEEECCC
TDTADAIGTAIQIKELANAGSELVRITVNTPEAAVAVPAIREQLDRMGVTVPLVGDFHYN
CCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHEEHHHHHHHHHHCCCEEEEEEEEEEC
GHLLLRDYPGCAESLSKYRINPGNVGQGAKRDTQFAQMIEAAAKYDKPVRIGVNWGSLDQ
CEEEEECCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCH
DLLARMMDENGARAQPWDAQSVMYEALIQSAIGSAERAVELGLGRDRIVLSCKVSGVQDL
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEECCCHHHH
IAVYRELGRRCGFALHLGLTEAGMGSKGIVASTAALGVLLQEGIGDTIRISLTPEPGASR
HHHHHHHHHHCCEEEEECCHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
TGEVIVGQEILQTMGLRSFAPMVIACPGCGRTTSTLFQELAMQIQTYLREQMPVWRKEYP
CCCEEECHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
GVEKMNVAVMGCIVNGPGESKHANIGISLPGSGENPAAPVFIDGEKVKTLRGERIAEEFQ
CCCCEEEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHH
QIVSDYVARNYGRTEALN
HHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDLSMQSEAQSPRSSQICSTEPVFGGHQPRRVSHAVDVRWGGQLVTIGGDAPVRVQSMTN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEEEEECCEEEEECCCCCEEEEECCC
TDTADAIGTAIQIKELANAGSELVRITVNTPEAAVAVPAIREQLDRMGVTVPLVGDFHYN
CCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHEEHHHHHHHHHHCCCEEEEEEEEEEC
GHLLLRDYPGCAESLSKYRINPGNVGQGAKRDTQFAQMIEAAAKYDKPVRIGVNWGSLDQ
CEEEEECCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCH
DLLARMMDENGARAQPWDAQSVMYEALIQSAIGSAERAVELGLGRDRIVLSCKVSGVQDL
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEECCCHHHH
IAVYRELGRRCGFALHLGLTEAGMGSKGIVASTAALGVLLQEGIGDTIRISLTPEPGASR
HHHHHHHHHHCCEEEEECCHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
TGEVIVGQEILQTMGLRSFAPMVIACPGCGRTTSTLFQELAMQIQTYLREQMPVWRKEYP
CCCEEECHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
GVEKMNVAVMGCIVNGPGESKHANIGISLPGSGENPAAPVFIDGEKVKTLRGERIAEEFQ
CCCCEEEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHH
QIVSDYVARNYGRTEALN
HHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA