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The map label for this gene is phbC [H]

Identifier: 116689812

GI number: 116689812

Start: 1987918

End: 1989783

Strand: Reverse

Name: phbC [H]

Synonym: Bcen2424_1791

Alternate gene names: 116689812

Gene position: 1989783-1987918 (Counterclockwise)

Preceding gene: 116689813

Following gene: 116689811

Centisome position: 57.11

GC content: 65.27

Gene sequence:

>1866_bases
ATGACAGCATCGAAAAATTCGTCGACGTCCGCGCATGCGGGCACTTCCGCGGGCAGCACCGGTTTCGATCCGGCCGCCCA
GCCGATGCAGCAGATGTTCGAGTCGTGGTTGAACGCGTGGCGTGGTTTTGCCGATCCGGCGCGTGCCGCGACGGCCTCGG
CCACCGTCAATCCGTTCGCGACATTCCAGTTCCCGACATCGTTTCCGTTCCAGCTGCCGTCGATGCCCGACCTCGGTGCG
ATGGCGTCGCCGTTCGCGGGGCTGAAGCTGCCAGTTGCGGCGATTCCGCCCGAACGGCTCCAGGCATTGCAGGCCGACTA
CGCGCGCGATTGCATGACGCTGATGCAGCAGGCGGCCGCCGCGAAGCTCGAGTCGCCGGAGTTGAAAGACCGCCGCTTCA
GCGGCGACGCATGGAAGGCGTCGCCCGCGCATGCGTTCGCGGCTGCGTGGTATCTGCTCAACGCGCGTTATCTGCAGGAG
CTGGCCGACGCGTTGCAGACCGATCCGAAGACGCGCGAGCGCATTCGTTTCACGGTCCAGCAATGGACGGCCGCCGCCGC
GCCGAGCAACTTCCTCGCACTCAATCCCGATGCACAGAAATCGATCCTCGAGACGCAGGGCGAAAGCCTGCGGCAGGGGA
TGATGAACCTGCTCGGCGACCTGCAGCGCGGCAAGATTTCGCAGACCGACGAATCGCAGTTCGTGGTCGGCAAGAACCTC
GGGTGTACCGCGGGCGCGGTCGTGTACGAGAACGACCTGATCCAGCTGATCCAGTACACGCCGAAGACGGACAAGGTGTT
CGAGCGGCCGCTGCTGATCGTGCCGCCGTGCATCAACAAGTTCTACATCCTCGACCTGCAACCCGAGAATTCGCTCGTCG
CGCACGCGCTGTCGAACGGCCATCAGGTGTTCCTCGTGTCGTGGCGCAACGCGGATGCGTCGGTCGCGCACAAGACGTGG
GACGACTACATGAACGAAGGGCTGCTCGCGGCGATCGACGCGGTGCAGCAGATCAGCGGCCGCGAGCAGATCAACACGCT
CGGCTTCTGCGTCGGCGGCACGATGCTCGCGACCGCGCTCGCGGTGCTCGCCGCCCGCGGCGAACATCCGGCTGCGTCGA
TGACATTGCTCACCGCGATGCTCGATTTCACCGACACGGGCATCCTCGACGTGTTCGTCGACGAAGCGCACGTGCAGATG
CGCGAGCAGACCATCGGCGGCAAGAACGGTACGCAACCGGGGCTGATGCGCGGCATGGAGTTCGCGAACACGTTCTCGTT
CCTGCGGCCGAACGATCTGGTGTGGAACTACGTCGTCGACAACTACCTGAAGGGCCGCACGCCCGCGCCGTTCGACCTGC
TGTACTGGAACAGCGATTCGACGAGCCTGCCGGGCCCGATGTACGCGTGGTACCTGCGCCATACCTATCTCGAAAACAAG
CTGCGCGAACCGGGCGCGTTGACCGTGTGCGGCGAATCGGTCGACCTGTCGCTGATCGACGTGCCGACCTTCATCTACGG
GTCGCGCGAGGATCACATCGTGCCGTGGCAGACCGCCTACGCATCGACGTCGATCCTGTCGGGTCCGCTGAAATTCGTGC
TCGGCGCGTCGGGGCACATCGCGGGCGTGATCAATCCGCCGGCGAAGAAGAAGCGCAGCTACTGGGTCAACGAGGGCGAC
CTGCCCGAATCCGCGGACGACTGGTTTGCCGGGGCGACCGAGCAGCCGGGAAGCTGGTGGACGACGTGGGTCGAGTGGCT
CGACGCATACGGCGGCCGCAAGGTCGTGCCGCCGTCCCAGCCGGGTTCCACGCAATTCCCGGTGATCGAGCCGGCACCCG
GGCGCTACGTGTTGCAGCGCGATTGA

Upstream 100 bases:

>100_bases
AGACGCCTCTCCAGAGCATGACGGCCCGGCTCGCAGCCGGCACGCCGCGCGCGAGCAGGATTTCACGATTGACGCTCACG
AATCACCGGTAAGGCAGGTA

Downstream 100 bases:

>100_bases
CGAAAACCGGACAGCGGCGCACGCTGTCCGGTGCACCGCAGTTTTTTTAACAGGGCCGGTAACGATGCGCCGTGTCGTGC
GGGCCTGGAGGAAAGGGAAA

Product: poly(R)-hydroxyalkanoic acid synthase, class I

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 621; Mature: 620

Protein sequence:

>621_residues
MTASKNSSTSAHAGTSAGSTGFDPAAQPMQQMFESWLNAWRGFADPARAATASATVNPFATFQFPTSFPFQLPSMPDLGA
MASPFAGLKLPVAAIPPERLQALQADYARDCMTLMQQAAAAKLESPELKDRRFSGDAWKASPAHAFAAAWYLLNARYLQE
LADALQTDPKTRERIRFTVQQWTAAAAPSNFLALNPDAQKSILETQGESLRQGMMNLLGDLQRGKISQTDESQFVVGKNL
GCTAGAVVYENDLIQLIQYTPKTDKVFERPLLIVPPCINKFYILDLQPENSLVAHALSNGHQVFLVSWRNADASVAHKTW
DDYMNEGLLAAIDAVQQISGREQINTLGFCVGGTMLATALAVLAARGEHPAASMTLLTAMLDFTDTGILDVFVDEAHVQM
REQTIGGKNGTQPGLMRGMEFANTFSFLRPNDLVWNYVVDNYLKGRTPAPFDLLYWNSDSTSLPGPMYAWYLRHTYLENK
LREPGALTVCGESVDLSLIDVPTFIYGSREDHIVPWQTAYASTSILSGPLKFVLGASGHIAGVINPPAKKKRSYWVNEGD
LPESADDWFAGATEQPGSWWTTWVEWLDAYGGRKVVPPSQPGSTQFPVIEPAPGRYVLQRD

Sequences:

>Translated_621_residues
MTASKNSSTSAHAGTSAGSTGFDPAAQPMQQMFESWLNAWRGFADPARAATASATVNPFATFQFPTSFPFQLPSMPDLGA
MASPFAGLKLPVAAIPPERLQALQADYARDCMTLMQQAAAAKLESPELKDRRFSGDAWKASPAHAFAAAWYLLNARYLQE
LADALQTDPKTRERIRFTVQQWTAAAAPSNFLALNPDAQKSILETQGESLRQGMMNLLGDLQRGKISQTDESQFVVGKNL
GCTAGAVVYENDLIQLIQYTPKTDKVFERPLLIVPPCINKFYILDLQPENSLVAHALSNGHQVFLVSWRNADASVAHKTW
DDYMNEGLLAAIDAVQQISGREQINTLGFCVGGTMLATALAVLAARGEHPAASMTLLTAMLDFTDTGILDVFVDEAHVQM
REQTIGGKNGTQPGLMRGMEFANTFSFLRPNDLVWNYVVDNYLKGRTPAPFDLLYWNSDSTSLPGPMYAWYLRHTYLENK
LREPGALTVCGESVDLSLIDVPTFIYGSREDHIVPWQTAYASTSILSGPLKFVLGASGHIAGVINPPAKKKRSYWVNEGD
LPESADDWFAGATEQPGSWWTTWVEWLDAYGGRKVVPPSQPGSTQFPVIEPAPGRYVLQRD
>Mature_620_residues
TASKNSSTSAHAGTSAGSTGFDPAAQPMQQMFESWLNAWRGFADPARAATASATVNPFATFQFPTSFPFQLPSMPDLGAM
ASPFAGLKLPVAAIPPERLQALQADYARDCMTLMQQAAAAKLESPELKDRRFSGDAWKASPAHAFAAAWYLLNARYLQEL
ADALQTDPKTRERIRFTVQQWTAAAAPSNFLALNPDAQKSILETQGESLRQGMMNLLGDLQRGKISQTDESQFVVGKNLG
CTAGAVVYENDLIQLIQYTPKTDKVFERPLLIVPPCINKFYILDLQPENSLVAHALSNGHQVFLVSWRNADASVAHKTWD
DYMNEGLLAAIDAVQQISGREQINTLGFCVGGTMLATALAVLAARGEHPAASMTLLTAMLDFTDTGILDVFVDEAHVQMR
EQTIGGKNGTQPGLMRGMEFANTFSFLRPNDLVWNYVVDNYLKGRTPAPFDLLYWNSDSTSLPGPMYAWYLRHTYLENKL
REPGALTVCGESVDLSLIDVPTFIYGSREDHIVPWQTAYASTSILSGPLKFVLGASGHIAGVINPPAKKKRSYWVNEGDL
PESADDWFAGATEQPGSWWTTWVEWLDAYGGRKVVPPSQPGSTQFPVIEPAPGRYVLQRD

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010963
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 68102; Mature: 67970

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASKNSSTSAHAGTSAGSTGFDPAAQPMQQMFESWLNAWRGFADPARAATASATVNPFA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCE
TFQFPTSFPFQLPSMPDLGAMASPFAGLKLPVAAIPPERLQALQADYARDCMTLMQQAAA
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHH
AKLESPELKDRRFSGDAWKASPAHAFAAAWYLLNARYLQELADALQTDPKTRERIRFTVQ
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
QWTAAAAPSNFLALNPDAQKSILETQGESLRQGMMNLLGDLQRGKISQTDESQFVVGKNL
HHHHHCCCCCEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCC
GCTAGAVVYENDLIQLIQYTPKTDKVFERPLLIVPPCINKFYILDLQPENSLVAHALSNG
CCCCCEEEEHHHHHHHHHCCCCCHHHHHCCCEEECCCCCCEEEEEECCCCHHHHHHHCCC
HQVFLVSWRNADASVAHKTWDDYMNEGLLAAIDAVQQISGREQINTLGFCVGGTMLATAL
CEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
AVLAARGEHPAASMTLLTAMLDFTDTGILDVFVDEAHVQMREQTIGGKNGTQPGLMRGME
HHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHCCCCCCCCHHHHHHHH
FANTFSFLRPNDLVWNYVVDNYLKGRTPAPFDLLYWNSDSTSLPGPMYAWYLRHTYLENK
HHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
LREPGALTVCGESVDLSLIDVPTFIYGSREDHIVPWQTAYASTSILSGPLKFVLGASGHI
CCCCCCEEEECCCCCEEEEECCHHEECCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCE
AGVINPPAKKKRSYWVNEGDLPESADDWFAGATEQPGSWWTTWVEWLDAYGGRKVVPPSQ
EEEECCCHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
PGSTQFPVIEPAPGRYVLQRD
CCCCCCCEECCCCCCEEEECC
>Mature Secondary Structure 
TASKNSSTSAHAGTSAGSTGFDPAAQPMQQMFESWLNAWRGFADPARAATASATVNPFA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCE
TFQFPTSFPFQLPSMPDLGAMASPFAGLKLPVAAIPPERLQALQADYARDCMTLMQQAAA
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHH
AKLESPELKDRRFSGDAWKASPAHAFAAAWYLLNARYLQELADALQTDPKTRERIRFTVQ
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
QWTAAAAPSNFLALNPDAQKSILETQGESLRQGMMNLLGDLQRGKISQTDESQFVVGKNL
HHHHHCCCCCEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCC
GCTAGAVVYENDLIQLIQYTPKTDKVFERPLLIVPPCINKFYILDLQPENSLVAHALSNG
CCCCCEEEEHHHHHHHHHCCCCCHHHHHCCCEEECCCCCCEEEEEECCCCHHHHHHHCCC
HQVFLVSWRNADASVAHKTWDDYMNEGLLAAIDAVQQISGREQINTLGFCVGGTMLATAL
CEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
AVLAARGEHPAASMTLLTAMLDFTDTGILDVFVDEAHVQMREQTIGGKNGTQPGLMRGME
HHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHCCCCCCCCHHHHHHHH
FANTFSFLRPNDLVWNYVVDNYLKGRTPAPFDLLYWNSDSTSLPGPMYAWYLRHTYLENK
HHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
LREPGALTVCGESVDLSLIDVPTFIYGSREDHIVPWQTAYASTSILSGPLKFVLGASGHI
CCCCCCEEEECCCCCEEEEECCHHEECCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCE
AGVINPPAKKKRSYWVNEGDLPESADDWFAGATEQPGSWWTTWVEWLDAYGGRKVVPPSQ
EEEECCCHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
PGSTQFPVIEPAPGRYVLQRD
CCCCCCCEECCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2670936; 1987116 [H]