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The map label for this gene is selU [H]

Identifier: 116689614

GI number: 116689614

Start: 1753238

End: 1754365

Strand: Direct

Name: selU [H]

Synonym: Bcen2424_1592

Alternate gene names: 116689614

Gene position: 1753238-1754365 (Clockwise)

Preceding gene: 116689613

Following gene: 116689615

Centisome position: 50.32

GC content: 67.38

Gene sequence:

>1128_bases
TTGAAGAACCTGATTGTCACCCTCGATCGCGCCGGCGAGTTCGACGAGATCATCGACGTGCGCACGCCGCTCGAGTTCGC
CGAGGACCATATCCCCGGCGCGCTGAACGCGCCCGTGCTCAGCAACGAAGAACGCGTGCTCGTCGGCACGATGTACCGCC
AGGTGTCGCCGTACGAGGCGACGCGCGTCGGCGCGGCGATCGTCGCGCGCAACATCGCGCGCCATCTCGACACGATGTTC
GCCGACCGGCCGCGCAACTGGCGGCCGCTGATCTACTGCTGGCGCGGCGGCAAGCGCTCGGGCTCGATGACGACCTGGTT
CAACCTGATCGGGTGGAAGGCGCGCCAGCTCGACGGCGGCTACAAGACGTACCGCCAGTCGGTATGCGCGACGCTCGACT
CGCTGCCGACACGCTTTCGCTACATCGCGCTCGTCGGCCATACGGGCTGCGGCAAGACGCGCCTGCTGAACGCGCTGCGC
GACGTGGGTGCCCAGACGCTCGATCTGGAAGCGCTCGCGTGCCATCGCGGCTCGCTGCTCGGCGCGCTGCCGGGCAAGCC
GCAACCGGCGCAGAAGGGGTTCGATTCGGGGCTCGTGGCGGCGCTTGGTCGTTTCGACCCCGAATGGCCGGTATTCGTGG
AGTCGGAAAGCCGCAGGATCGGGCTCGTCCAGCTGCCGATCGCGCTGATCGAAGCGTTTCACGCGGGCCCATGGGTGCAG
GTCGAAGCGGCTCACGACGAGCGGATCGCGTTCCTGCTCGATGATTACGCGCACCTGTTCGACGAGCCGGATGCGTTCAA
GGCACAGCTTCATCGGCTGATCGGCCTGCACAGCCGCGAAGAAGTGACGCGCTGGAAGGGGCTGATCGATGCCGGCGCGC
GGGCCGATTTGTTCGCCGAACTGATCGACAGGCATTACGATCCCGCCTACGCGCGCACTTACCGCGCGACGTACAACAAG
CCGAACCGGGCGCTGACCTTCACGTTCCGGCCCAACGCGGCCGACGTGCGCGAGCAGGCGCGCCGGCTGCTGGCCGAACT
TGCGCAAGCCGGGTTGCCCGCATCGGCCGCGCTCGCCGCCGGCACGACATCCGAAACCGACAAAGACCAATCGACCACCA
CACGATGA

Upstream 100 bases:

>100_bases
GCCGCGCGAGGCCCGGGACGCCGGCCCGTGAGATAATCGTCAATCCGCCTTCCGTGCCCATGCGGCTCCTCGAGCCGCAT
CGCCCCTGTTCGACGCCAAA

Downstream 100 bases:

>100_bases
CGACCACACGCACTCAACCGAATCCGGCCCTCGGCTGGATGACCTTCCTGCTGATCGCGGTCGCGGGACTGTTCTACGTG
AAATGGTTCCCGTACTACAA

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MKNLIVTLDRAGEFDEIIDVRTPLEFAEDHIPGALNAPVLSNEERVLVGTMYRQVSPYEATRVGAAIVARNIARHLDTMF
ADRPRNWRPLIYCWRGGKRSGSMTTWFNLIGWKARQLDGGYKTYRQSVCATLDSLPTRFRYIALVGHTGCGKTRLLNALR
DVGAQTLDLEALACHRGSLLGALPGKPQPAQKGFDSGLVAALGRFDPEWPVFVESESRRIGLVQLPIALIEAFHAGPWVQ
VEAAHDERIAFLLDDYAHLFDEPDAFKAQLHRLIGLHSREEVTRWKGLIDAGARADLFAELIDRHYDPAYARTYRATYNK
PNRALTFTFRPNAADVREQARRLLAELAQAGLPASAALAAGTTSETDKDQSTTTR

Sequences:

>Translated_375_residues
MKNLIVTLDRAGEFDEIIDVRTPLEFAEDHIPGALNAPVLSNEERVLVGTMYRQVSPYEATRVGAAIVARNIARHLDTMF
ADRPRNWRPLIYCWRGGKRSGSMTTWFNLIGWKARQLDGGYKTYRQSVCATLDSLPTRFRYIALVGHTGCGKTRLLNALR
DVGAQTLDLEALACHRGSLLGALPGKPQPAQKGFDSGLVAALGRFDPEWPVFVESESRRIGLVQLPIALIEAFHAGPWVQ
VEAAHDERIAFLLDDYAHLFDEPDAFKAQLHRLIGLHSREEVTRWKGLIDAGARADLFAELIDRHYDPAYARTYRATYNK
PNRALTFTFRPNAADVREQARRLLAELAQAGLPASAALAAGTTSETDKDQSTTTR
>Mature_375_residues
MKNLIVTLDRAGEFDEIIDVRTPLEFAEDHIPGALNAPVLSNEERVLVGTMYRQVSPYEATRVGAAIVARNIARHLDTMF
ADRPRNWRPLIYCWRGGKRSGSMTTWFNLIGWKARQLDGGYKTYRQSVCATLDSLPTRFRYIALVGHTGCGKTRLLNALR
DVGAQTLDLEALACHRGSLLGALPGKPQPAQKGFDSGLVAALGRFDPEWPVFVESESRRIGLVQLPIALIEAFHAGPWVQ
VEAAHDERIAFLLDDYAHLFDEPDAFKAQLHRLIGLHSREEVTRWKGLIDAGARADLFAELIDRHYDPAYARTYRATYNK
PNRALTFTFRPNAADVREQARRLLAELAQAGLPASAALAAGTTSETDKDQSTTTR

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA [H]

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1786712, Length=352, Percent_Identity=29.2613636363636, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR017582 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: NA

Molecular weight: Translated: 41779; Mature: 41779

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLIVTLDRAGEFDEIIDVRTPLEFAEDHIPGALNAPVLSNEERVLVGTMYRQVSPYEA
CCCEEEEECCCCCHHHHHHCCCCHHHHHHCCCCCCCCCEECCCCEEEEEHHHHHCCCHHH
TRVGAAIVARNIARHLDTMFADRPRNWRPLIYCWRGGKRSGSMTTWFNLIGWKARQLDGG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCHHHHCCCH
YKTYRQSVCATLDSLPTRFRYIALVGHTGCGKTRLLNALRDVGAQTLDLEALACHRGSLL
HHHHHHHHHHHHHHCCHHEEEEEEEECCCCCHHHHHHHHHHCCCCEECHHHHHHCCCCEE
GALPGKPQPAQKGFDSGLVAALGRFDPEWPVFVESESRRIGLVQLPIALIEAFHAGPWVQ
ECCCCCCCHHHCCCCCHHHHHHHCCCCCCCEEEECCCCEEEEEEHHHHHHHHHCCCCCEE
VEAAHDERIAFLLDDYAHLFDEPDAFKAQLHRLIGLHSREEVTRWKGLIDAGARADLFAE
EECCCCCEEHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHH
LIDRHYDPAYARTYRATYNKPNRALTFTFRPNAADVREQARRLLAELAQAGLPASAALAA
HHHCCCCCHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHCC
GTTSETDKDQSTTTR
CCCCCCCCCCCCCCC
>Mature Secondary Structure
MKNLIVTLDRAGEFDEIIDVRTPLEFAEDHIPGALNAPVLSNEERVLVGTMYRQVSPYEA
CCCEEEEECCCCCHHHHHHCCCCHHHHHHCCCCCCCCCEECCCCEEEEEHHHHHCCCHHH
TRVGAAIVARNIARHLDTMFADRPRNWRPLIYCWRGGKRSGSMTTWFNLIGWKARQLDGG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCHHHHCCCH
YKTYRQSVCATLDSLPTRFRYIALVGHTGCGKTRLLNALRDVGAQTLDLEALACHRGSLL
HHHHHHHHHHHHHHCCHHEEEEEEEECCCCCHHHHHHHHHHCCCCEECHHHHHHCCCCEE
GALPGKPQPAQKGFDSGLVAALGRFDPEWPVFVESESRRIGLVQLPIALIEAFHAGPWVQ
ECCCCCCCHHHCCCCCHHHHHHHCCCCCCCEEEECCCCEEEEEEHHHHHHHHHCCCCCEE
VEAAHDERIAFLLDDYAHLFDEPDAFKAQLHRLIGLHSREEVTRWKGLIDAGARADLFAE
EECCCCCEEHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHH
LIDRHYDPAYARTYRATYNKPNRALTFTFRPNAADVREQARRLLAELAQAGLPASAALAA
HHHCCCCCHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHCC
GTTSETDKDQSTTTR
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]