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The map label for this gene is sucB [H]

Identifier: 116689531

GI number: 116689531

Start: 1668736

End: 1670016

Strand: Direct

Name: sucB [H]

Synonym: Bcen2424_1509

Alternate gene names: 116689531

Gene position: 1668736-1670016 (Clockwise)

Preceding gene: 116689530

Following gene: 116689532

Centisome position: 47.9

GC content: 65.73

Gene sequence:

>1281_bases
ATGGCTATCGTAGAAGTCAAAGTCCCCCAGTTGTCGGAGTCGGTGTCGGAAGCCACCATGCTGCAGTGGAAGAAGAAGCC
GGGCGAAGCAGTCGCGCAGGACGAAATCCTGATCGAACTCGAGACCGACAAGGTCGTCCTCGAAGTGCCGGCACCGGCAG
CGGGCGTGCTCGCGCAAGTGCTGCAGAACGACGGTGACACGGTCGTGGCCGACCAGATCATCGCGACGATCGACACCGAA
GCGAAGGCAGGTGCTGCCGAAGCCGCGGCAGGCGCCGCCGAAGTCAAGCCGGCAGCCGCGCCTGCAGCAGCAGCCGCACC
GGCCGCTCAGCCGGCAGCCGCAACGGCATCGAGCTCGGCCGCCGCATCGCCGGCCGCAGCGAAGCTGCTGGCCGAGAAGG
GCCTGTCGACGGGCGACGTCGCAGGTTCGGGCCGCGACGGCCGCGTCACGAAGGGCGACGCGCTGGCAGCAGGCAGCGCA
CCGAAGGCTGCTCCGGCCGCCGCACCGGCGAAGACCGCCGCCGCGAAGCCGGCGCTGCCGGAAGTGAAGGTGCCGGCGTC
GGCAGCGACCTGGCTGAACGACCGCCCGGAACAGCGTGTGCCGATGTCGCGCCTGCGTGCGCGTATCGCCGAGCGTCTGC
TCGAATCGCAGCAGACCAACGCGATCCTGACGACGTTCAACGAAGTGAACATGGCTCCGGTCATGGAACTGCGCAACAAG
TACAAGGACAAGTTCGAGAAGGAACACGGCGTGAAGCTCGGCTTCATGTCGTTCTTCGTGAAGGCGGCCGTCCATGCGCT
GAAGAAGTTCCCGCTCGTGAACGCGTCGATCGACGGTAACGACATCGTCTACCACGGCTACTTCGACATCGGTATCGCGG
TCGGTTCGCCGCGCGGCCTGGTCGTGCCGATCCTGCGCAACGCGGATCAACTGAGCCTCGCCGAGATCGAGAAGAAGATC
GCCGAATTCGGCCAGAAGGCGAAGGACGGCAAGCTGTCGATCGAGGAAATGACGGGCGGTACGTTCTCGATCTCGAACGG
CGGCGTGTTCGGCTCGATGCTGTCGACCCCGATCATCAACCCGCCGCAATCGGCCATCCTCGGCGTGCACGCGACGAAGG
AGCGCCCGGTCGTCGAGAACGGCCAGATCGTGATCCGCCCGATCAACTACCTCGCGCTGTCGTACGACCACCGCATCATC
GACGGCCGCGAAGCCGTGCTGTCGCTCGTCGCGATGAAGGATGCGCTGGAAGATCCGGCACGCCTGCTGCTCGACCTGTA
A

Upstream 100 bases:

>100_bases
TCCCGTTCCTTTTGGCCCGCCGGGCGCGTCGCGCGCCGCACCGGCCGAAGGAATCGCACGAACCTCGTCATACCCCAGAT
TAAGCATCCAGGAAAATCAC

Downstream 100 bases:

>100_bases
GCCGAGTCTCACCGCATCGACCCGTTCCGCAGGTTCGCGCCCACGTGCGAACCTGCGGGCGTACAAGTAGAAAGGATTGT
CATGTCCAAGGAATTTGACG

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MAIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLAQVLQNDGDTVVADQIIATIDTE
AKAGAAEAAAGAAEVKPAAAPAAAAAPAAQPAAATASSSAAASPAAAKLLAEKGLSTGDVAGSGRDGRVTKGDALAAGSA
PKAAPAAAPAKTAAAKPALPEVKVPASAATWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMAPVMELRNK
YKDKFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQLSLAEIEKKI
AEFGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQSAILGVHATKERPVVENGQIVIRPINYLALSYDHRII
DGREAVLSLVAMKDALEDPARLLLDL

Sequences:

>Translated_426_residues
MAIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLAQVLQNDGDTVVADQIIATIDTE
AKAGAAEAAAGAAEVKPAAAPAAAAAPAAQPAAATASSSAAASPAAAKLLAEKGLSTGDVAGSGRDGRVTKGDALAAGSA
PKAAPAAAPAKTAAAKPALPEVKVPASAATWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMAPVMELRNK
YKDKFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQLSLAEIEKKI
AEFGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQSAILGVHATKERPVVENGQIVIRPINYLALSYDHRII
DGREAVLSLVAMKDALEDPARLLLDL
>Mature_425_residues
AIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLAQVLQNDGDTVVADQIIATIDTEA
KAGAAEAAAGAAEVKPAAAPAAAAAPAAQPAAATASSSAAASPAAAKLLAEKGLSTGDVAGSGRDGRVTKGDALAAGSAP
KAAPAAAPAKTAAAKPALPEVKVPASAATWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMAPVMELRNKY
KDKFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQLSLAEIEKKIA
EFGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQSAILGVHATKERPVVENGQIVIRPINYLALSYDHRIID
GREAVLSLVAMKDALEDPARLLLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=443, Percent_Identity=31.1512415349887, Blast_Score=172, Evalue=6e-43,
Organism=Homo sapiens, GI110671329, Length=437, Percent_Identity=27.6887871853547, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI203098753, Length=458, Percent_Identity=28.82096069869, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI203098816, Length=458, Percent_Identity=28.82096069869, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=37.888198757764, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1786946, Length=429, Percent_Identity=53.1468531468531, Blast_Score=440, Evalue=1e-125,
Organism=Escherichia coli, GI1786305, Length=430, Percent_Identity=32.093023255814, Blast_Score=170, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI25146366, Length=431, Percent_Identity=43.8515081206497, Blast_Score=328, Evalue=4e-90,
Organism=Caenorhabditis elegans, GI17560088, Length=452, Percent_Identity=31.6371681415929, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17537937, Length=446, Percent_Identity=26.457399103139, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17538894, Length=326, Percent_Identity=32.5153374233129, Blast_Score=145, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6320352, Length=421, Percent_Identity=43.4679334916865, Blast_Score=332, Evalue=7e-92,
Organism=Saccharomyces cerevisiae, GI6324258, Length=454, Percent_Identity=29.7356828193833, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24645909, Length=242, Percent_Identity=54.9586776859504, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI18859875, Length=435, Percent_Identity=29.1954022988506, Blast_Score=158, Evalue=6e-39,
Organism=Drosophila melanogaster, GI24582497, Length=228, Percent_Identity=34.2105263157895, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI20129315, Length=228, Percent_Identity=34.2105263157895, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 44401; Mature: 44270

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLAQV
CEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEEEECCCCHHHHHHHH
LQNDGDTVVADQIIATIDTEAKAGAAEAAAGAAEVKPAAAPAAAAAPAAQPAAATASSSA
HHCCCCEEEHHHHHHHCCCCCCCCCHHHHCCCHHCCCCCCCCHHCCCCCCCCHHCCCCCC
AASPAAAKLLAEKGLSTGDVAGSGRDGRVTKGDALAAGSAPKAAPAAAPAKTAAAKPALP
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCHHHCCCCCCC
EVKVPASAATWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMAPVMELRNK
CCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHH
YKDKFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEEEEEEECCCCCE
VVPILRNADQLSLAEIEKKIAEFGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIIN
EEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCEEHHHHCCCCCC
PPQSAILGVHATKERPVVENGQIVIRPINYLALSYDHRIIDGREAVLSLVAMKDALEDPA
CCHHHEEEEECCCCCCCCCCCCEEEEECCEEEEECCCEEECCHHHHHHHHHHHHHHCCHH
RLLLDL
HHHHCC
>Mature Secondary Structure 
AIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLAQV
EEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEEEECCCCHHHHHHHH
LQNDGDTVVADQIIATIDTEAKAGAAEAAAGAAEVKPAAAPAAAAAPAAQPAAATASSSA
HHCCCCEEEHHHHHHHCCCCCCCCCHHHHCCCHHCCCCCCCCHHCCCCCCCCHHCCCCCC
AASPAAAKLLAEKGLSTGDVAGSGRDGRVTKGDALAAGSAPKAAPAAAPAKTAAAKPALP
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCHHHCCCCCCC
EVKVPASAATWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMAPVMELRNK
CCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHH
YKDKFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEEEEEEECCCCCE
VVPILRNADQLSLAEIEKKIAEFGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIIN
EEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCEEHHHHCCCCCC
PPQSAILGVHATKERPVVENGQIVIRPINYLALSYDHRIIDGREAVLSLVAMKDALEDPA
CCHHHEEEEECCCCCCCCCCCCEEEEECCEEEEECCCEEECCHHHHHHHHHHHHHHCCHH
RLLLDL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]