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The map label for this gene is odhL [H]

Identifier: 116689532

GI number: 116689532

Start: 1670098

End: 1671528

Strand: Direct

Name: odhL [H]

Synonym: Bcen2424_1510

Alternate gene names: 116689532

Gene position: 1670098-1671528 (Clockwise)

Preceding gene: 116689531

Following gene: 116689533

Centisome position: 47.94

GC content: 64.78

Gene sequence:

>1431_bases
ATGTCCAAGGAATTTGACGTCGTCGTGATCGGCGCCGGCCCTGGCGGCTACATCGCCGCGATTCGCGCCGCGCAGCTCGG
CAAGACCGTTGCCTGTATCGAGAAGTGGAAGAACCCGGCCGGCGCGCTGAAGCTCGGCGGCACCTGCCTGAACGTCGGTT
GCATCCCGTCGAAGGCGCTGCTCGCGTCGTCGGAAGAGTTCGAGAACACGTCGCACCACCTGGCCGACCACGGCATCACG
GTCGACGGCGTGAAGATCGACGTCGCGAAGATGCTCGGCCGCAAGGATGCGATCGTCGAGAAGATGACGAGCGGGATCGA
GTTCCTGTTCAAGAAGAACAAGATCACCTGGCTGAAGGGCCATGGCAAGTTCACCGGCAAGACCGACGCCGGCGTGCAGA
TCGAAGTGAGCGGCGAGGGTGAAGCCGAAGTCGTCACCGCGAAGAACGTGATCATCGCGACGGGCTCGAAGGCGCGTCAC
CTGCCGAACATCCCGGTCGACAACAAGATCGTGTCGGACAACGAAGGTGCGCTGACGTTCGACTCGGTGCCGAAGAAGCT
CGCCGTGATCGGCGCGGGCGTGATCGGCCTCGAGCTCGGCTCGGTGTGGCGTCGCCTCGGTGCCGACGTGACGGTGCTCG
AAGCGCTGCCGGCGTTCCTCGGCGCAGCCGACGAAGCGCTCGCGAAGGAAGCGGCCAAGCTGTTCAAGAAGCAGGGCCTC
GACATCCATCTCGGCGTGAAGATCGGCGACGTGAAGACGACCGCCGACGGTGTGTCGATCGTCTACACGGACAAGGACGG
CAACGCGCAGACGCTCGACGCCGACCGCCTGATCGTGTCGGTCGGCCGCCTGCCGAACACCGACAACCTCGGCCTCGAGG
CGATCGGCCTGAAGGCGAACGAGCGTGGTTTCATCGACGTGGACGACCACTGCCGCACGGCGGTGCCGAACGTGTACGCG
ATCGGCGACGTGGTGCGCGGCCCGATGCTCGCGCACAAGGCAGAAGACGAAGGCGTGCTGGTCGCGGAAGTGATCGACGG
CCAGAAGCCGCACATCGACTACAACTGCATTCCGTGGGTGATCTACACGTACCCGGAAATCGCATGGGTCGGCAAGACGG
AGCAGCAGCTGAAGGCGGAAGGCCGCGAGATCAAGACGGGCAAGTTCCCGTTCTCGATCAACGGCCGCGCGCTCGGCATG
AACGCGCCGGACGGCTTCGTGAAGATGATCGCGGATGCGAAGACCGACGAGCTGCTCGGCGTGCACGTGATCGCCGCGAA
CGCGTCGGACCTGATCGCGGAAGCCGTGGTCGCGATGGAATTCAAGGCGGCGTCGGAAGACATCGCCCGCATCTGCCATC
CGCACCCGTCGATGTCGGAAGTGATGCGCGAAGCGGCGCTCGCGGTCGACAAGCGCTCGCTGAACAGCTGA

Upstream 100 bases:

>100_bases
CCTGCTGCTCGACCTGTAAGCCGAGTCTCACCGCATCGACCCGTTCCGCAGGTTCGCGCCCACGTGCGAACCTGCGGGCG
TACAAGTAGAAAGGATTGTC

Downstream 100 bases:

>100_bases
GCGCGGTATAGCGCCGGCCGGCCATCGGCTGGCGCAACCGTCTCATGACGAAGGCGGGCGGGTTTTTCCCGCTCGCCTTT
GTTTTTTCCGGTTTGCCCCG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MSKEFDVVVIGAGPGGYIAAIRAAQLGKTVACIEKWKNPAGALKLGGTCLNVGCIPSKALLASSEEFENTSHHLADHGIT
VDGVKIDVAKMLGRKDAIVEKMTSGIEFLFKKNKITWLKGHGKFTGKTDAGVQIEVSGEGEAEVVTAKNVIIATGSKARH
LPNIPVDNKIVSDNEGALTFDSVPKKLAVIGAGVIGLELGSVWRRLGADVTVLEALPAFLGAADEALAKEAAKLFKKQGL
DIHLGVKIGDVKTTADGVSIVYTDKDGNAQTLDADRLIVSVGRLPNTDNLGLEAIGLKANERGFIDVDDHCRTAVPNVYA
IGDVVRGPMLAHKAEDEGVLVAEVIDGQKPHIDYNCIPWVIYTYPEIAWVGKTEQQLKAEGREIKTGKFPFSINGRALGM
NAPDGFVKMIADAKTDELLGVHVIAANASDLIAEAVVAMEFKAASEDIARICHPHPSMSEVMREAALAVDKRSLNS

Sequences:

>Translated_476_residues
MSKEFDVVVIGAGPGGYIAAIRAAQLGKTVACIEKWKNPAGALKLGGTCLNVGCIPSKALLASSEEFENTSHHLADHGIT
VDGVKIDVAKMLGRKDAIVEKMTSGIEFLFKKNKITWLKGHGKFTGKTDAGVQIEVSGEGEAEVVTAKNVIIATGSKARH
LPNIPVDNKIVSDNEGALTFDSVPKKLAVIGAGVIGLELGSVWRRLGADVTVLEALPAFLGAADEALAKEAAKLFKKQGL
DIHLGVKIGDVKTTADGVSIVYTDKDGNAQTLDADRLIVSVGRLPNTDNLGLEAIGLKANERGFIDVDDHCRTAVPNVYA
IGDVVRGPMLAHKAEDEGVLVAEVIDGQKPHIDYNCIPWVIYTYPEIAWVGKTEQQLKAEGREIKTGKFPFSINGRALGM
NAPDGFVKMIADAKTDELLGVHVIAANASDLIAEAVVAMEFKAASEDIARICHPHPSMSEVMREAALAVDKRSLNS
>Mature_475_residues
SKEFDVVVIGAGPGGYIAAIRAAQLGKTVACIEKWKNPAGALKLGGTCLNVGCIPSKALLASSEEFENTSHHLADHGITV
DGVKIDVAKMLGRKDAIVEKMTSGIEFLFKKNKITWLKGHGKFTGKTDAGVQIEVSGEGEAEVVTAKNVIIATGSKARHL
PNIPVDNKIVSDNEGALTFDSVPKKLAVIGAGVIGLELGSVWRRLGADVTVLEALPAFLGAADEALAKEAAKLFKKQGLD
IHLGVKIGDVKTTADGVSIVYTDKDGNAQTLDADRLIVSVGRLPNTDNLGLEAIGLKANERGFIDVDDHCRTAVPNVYAI
GDVVRGPMLAHKAEDEGVLVAEVIDGQKPHIDYNCIPWVIYTYPEIAWVGKTEQQLKAEGREIKTGKFPFSINGRALGMN
APDGFVKMIADAKTDELLGVHVIAANASDLIAEAVVAMEFKAASEDIARICHPHPSMSEVMREAALAVDKRSLNS

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=477, Percent_Identity=47.5890985324948, Blast_Score=430, Evalue=1e-120,
Organism=Homo sapiens, GI50301238, Length=470, Percent_Identity=28.2978723404255, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI22035672, Length=474, Percent_Identity=27.8481012658228, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI33519430, Length=467, Percent_Identity=25.4817987152034, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI33519428, Length=467, Percent_Identity=25.4817987152034, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI33519426, Length=467, Percent_Identity=25.4817987152034, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI148277065, Length=467, Percent_Identity=25.4817987152034, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI148277071, Length=467, Percent_Identity=25.4817987152034, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI291045266, Length=479, Percent_Identity=24.8434237995825, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI291045268, Length=474, Percent_Identity=23.8396624472574, Blast_Score=102, Evalue=9e-22,
Organism=Escherichia coli, GI1786307, Length=468, Percent_Identity=38.8888888888889, Blast_Score=320, Evalue=1e-88,
Organism=Escherichia coli, GI87082354, Length=475, Percent_Identity=31.5789473684211, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1789915, Length=465, Percent_Identity=30.3225806451613, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI87081717, Length=474, Percent_Identity=28.4810126582279, Blast_Score=162, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI32565766, Length=469, Percent_Identity=47.9744136460554, Blast_Score=438, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17557007, Length=488, Percent_Identity=28.2786885245902, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI71982272, Length=489, Percent_Identity=27.4028629856851, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI71983419, Length=464, Percent_Identity=28.0172413793103, Blast_Score=125, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI71983429, Length=464, Percent_Identity=28.0172413793103, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17559934, Length=196, Percent_Identity=30.6122448979592, Blast_Score=72, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6321091, Length=482, Percent_Identity=46.2655601659751, Blast_Score=412, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6325240, Length=477, Percent_Identity=29.979035639413, Blast_Score=216, Evalue=9e-57,
Organism=Saccharomyces cerevisiae, GI6325166, Length=473, Percent_Identity=27.2727272727273, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI21358499, Length=470, Percent_Identity=49.7872340425532, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24640549, Length=490, Percent_Identity=28.1632653061224, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24640553, Length=490, Percent_Identity=28.1632653061224, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24640551, Length=490, Percent_Identity=28.1632653061224, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI17737741, Length=489, Percent_Identity=26.5848670756646, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50521; Mature: 50389

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEFDVVVIGAGPGGYIAAIRAAQLGKTVACIEKWKNPAGALKLGGTCLNVGCIPSKAL
CCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCEEECCEEEEECCCCCHHH
LASSEEFENTSHHLADHGITVDGVKIDVAKMLGRKDAIVEKMTSGIEFLFKKNKITWLKG
HCCCHHHCCCHHHHHHCCEEEECEEEEHHHHCCCHHHHHHHHHHHHHEEEECCCEEEEEC
HGKFTGKTDAGVQIEVSGEGEAEVVTAKNVIIATGSKARHLPNIPVDNKIVSDNEGALTF
CCEECCCCCCCEEEEECCCCCEEEEEECEEEEECCCCCCCCCCCCCCCEEECCCCCCEEC
DSVPKKLAVIGAGVIGLELGSVWRRLGADVTVLEALPAFLGAADEALAKEAAKLFKKQGL
CCCCCEEHHHCCCHHHHHHHHHHHHHCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
DIHLGVKIGDVKTTADGVSIVYTDKDGNAQTLDADRLIVSVGRLPNTDNLGLEAIGLKAN
EEEEEEEECCEEECCCCEEEEEECCCCCCEEECCCCEEEEECCCCCCCCCCEEEEEEECC
ERGFIDVDDHCRTAVPNVYAIGDVVRGPMLAHKAEDEGVLVAEVIDGQKPHIDYNCIPWV
CCCCEECCCCHHHCCCCEEEEHHHHCCCHHEECCCCCCEEEEEECCCCCCCCCCCCCCEE
IYTYPEIAWVGKTEQQLKAEGREIKTGKFPFSINGRALGMNAPDGFVKMIADAKTDELLG
EEECCCEEEECCCHHHHHHCCCEEECCCCCEEECCEEEECCCCHHHHHHHHCCCCCCEEE
VHVIAANASDLIAEAVVAMEFKAASEDIARICHPHPSMSEVMREAALAVDKRSLNS
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKEFDVVVIGAGPGGYIAAIRAAQLGKTVACIEKWKNPAGALKLGGTCLNVGCIPSKAL
CCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCEEECCEEEEECCCCCHHH
LASSEEFENTSHHLADHGITVDGVKIDVAKMLGRKDAIVEKMTSGIEFLFKKNKITWLKG
HCCCHHHCCCHHHHHHCCEEEECEEEEHHHHCCCHHHHHHHHHHHHHEEEECCCEEEEEC
HGKFTGKTDAGVQIEVSGEGEAEVVTAKNVIIATGSKARHLPNIPVDNKIVSDNEGALTF
CCEECCCCCCCEEEEECCCCCEEEEEECEEEEECCCCCCCCCCCCCCCEEECCCCCCEEC
DSVPKKLAVIGAGVIGLELGSVWRRLGADVTVLEALPAFLGAADEALAKEAAKLFKKQGL
CCCCCEEHHHCCCHHHHHHHHHHHHHCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
DIHLGVKIGDVKTTADGVSIVYTDKDGNAQTLDADRLIVSVGRLPNTDNLGLEAIGLKAN
EEEEEEEECCEEECCCCEEEEEECCCCCCEEECCCCEEEEECCCCCCCCCCEEEEEEECC
ERGFIDVDDHCRTAVPNVYAIGDVVRGPMLAHKAEDEGVLVAEVIDGQKPHIDYNCIPWV
CCCCEECCCCHHHCCCCEEEEHHHHCCCHHEECCCCCCEEEEEECCCCCCCCCCCCCCEE
IYTYPEIAWVGKTEQQLKAEGREIKTGKFPFSINGRALGMNAPDGFVKMIADAKTDELLG
EEECCCEEEECCCHHHHHHCCCEEECCCCCEEECCEEEECCCCHHHHHHHHCCCCCCEEE
VHVIAANASDLIAEAVVAMEFKAASEDIARICHPHPSMSEVMREAALAVDKRSLNS
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]