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The map label for this gene is iolD [H]

Identifier: 116689444

GI number: 116689444

Start: 1567271

End: 1569154

Strand: Reverse

Name: iolD [H]

Synonym: Bcen2424_1422

Alternate gene names: 116689444

Gene position: 1569154-1567271 (Counterclockwise)

Preceding gene: 116689445

Following gene: 116689443

Centisome position: 45.04

GC content: 70.49

Gene sequence:

>1884_bases
ATGACCACCACCGTAAGACTGACCGTCAGCCAGGCCCTCGTGCGCTACCTGGCCGCCCTGCGCGCCGAAGTCGTCCAGCC
CGACGGCCGCACCGAAATCCTGCCGTACTGCGGCGGCGTGTTCGCGATCTTCGGCCACGGCAACGTGGCCGGGCTCGGCG
AAGCGCTCCATGCGGAGAAAGACCGGCTGCCGACGTTTCGCGCACACAACGAGCAAGGGATGGCCAACGCGGCCGTCGCG
TTCGCGAAAGCGAATTTCCGCCAGCGGATGATGGCCGCGACGTCGAGCATCGGCCCCGGCGCCACCAACATGCTGACCTC
CGCCGCGCTCGCGCACGTCGGCCGCTTGCCGCTGCTGCTGCTGCCCGGCGACGTGTTCGTGTCGCGGCTGCCCGACCCGG
TGCTGCAGCAGGTCGAGGATTTCGAACAGGGCGACGTCAGCGCGAACGACTGCTTCCGGCCCGTGACGCGCTACTTCGAC
CGCATCACGTCGCCGGAGCAACTGCTCGTCGCGCTGCCGCGCGCGATCCAGGTGATGACCGATCCCGCACAATGCGGGCC
CGTGTGTCTCGCGCTGCCGCAGGACGTGCAGACGTTCGCGTACGACTGGCCCGAGGATTTCTTCGCGCCGCCGCTGATCC
GGATGCGCCGGCCGCCGGCCGACGCGCTCGAACTCGCCGATGCGCTCGACGTGCTGAAGGCCGCGAAGAAGCCGCTGATC
GTCGCCGGCGGCGGCGTGCTGTACAGCCAGGCGTGGGATGCGCTGCGCACGTTCGCCGATACGCACGGCGTGCCGGTCGC
CGAATCGCAGGCCGGCAAGGGCAGCCTCGCGTGGGATCACCCGCTGAACCTCGGCGCGATCGGCGTGACGGGCTCGCCCG
CCGCGAACCGCGCGGCCGCGCAGGCCGACGTCGTGTTCGCGGTCGGCACGCGGCTGCAGGATTTCACGACCGGCTCGCAC
GCGCTCTACGGCGACGCGACGCTGCTGAGCCTGAACGTGCAGCCGTTCGACGCGGGCAAGAAACGCGGCCGGCAACTGGT
GGCCGATGCGCGCACCGGCCTCGGGCAACTGTCGGCCGCGCTCGCCGGCTGGCGCGCCGATCCGGCGTGGACCGCCGCGA
ACCGCGACCAGGCCGCCGCGTGGAACGCGCGCGTGACGGACCTGACCACGCGGATTCCGAAGGACACGCTGCCGTACGAC
GCCGAAGTGATCGGCGCGGTGCGCGACTCCGCGGCCGACGCGGGGCGCGACAGCGCGCGCGACGATCTCGTCGTCTGCGC
GGCCGGCACGCTGCCGGCCGAACTGCACAAGCTGTGGCGCAGCGGCGTACCGGGCAACTATCACATGGACTATGCGTATT
CGTGCATGGGCTACGAAGTCGCGGGCGGCCTCGGCGCGAAGCTCGCGCGACCCGAGCGCGAAGTGATCGTGATCGTCGGC
GACGGCTCGTACATGATGCTCAACGCGGAACTCGCGACCTCCGTGATGCTCGGCCGCAAGATCATCGTCGTGATCCTCGA
CAACCGCGGCTACGGCTGCATCGAGCGGCTGCAACTCAATTGCGGCGGCGCGAGCTTCAACAACATGCTCGACGACTGCG
TGCCCGAAGGCGGCGAACGCTCGACGATCGACTTCGCGATGCATGCGCGCGCGATGGGCGCCGAAGCCGTGCACGTGCGC
GACGTCGGCGAGCTGCGCCACGAAATGAAGCGCGCGCGTGCGGCGACGACCAGCCAGGTGCTCGTGATCGACACCACGCA
CCAGCGCACGACCGACGACGGCGGCGCGTGGTGGGAAGTCGCGGTGCCGCAGGTGTCCGAACGCGCCGGCGTCGTGGACG
CACACCGCGCGTATCTCGACGCGAAAACCCGCCAGCGGCGCTGA

Upstream 100 bases:

>100_bases
CTGGTCGACGCGTGGCTGGGCCGCCGTACCGCCGCGCGCGCCGCCGCGGCCTGATGCCCGTGTTCGTGACGACCACCCGA
CTTCTCTCTATCGACCGACG

Downstream 100 bases:

>100_bases
TCCGCTCACCCTTTCCATCGGCCGCGCCGAGACAACACCATTCGAACCAGGAAACACGTCATGAGCTGGAACGTCCGCAT
CGGTATCAATCCCCTGTCGT

Product: thiamine pyrophosphate binding domain-containing protein

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase [H]

Number of amino acids: Translated: 627; Mature: 626

Protein sequence:

>627_residues
MTTTVRLTVSQALVRYLAALRAEVVQPDGRTEILPYCGGVFAIFGHGNVAGLGEALHAEKDRLPTFRAHNEQGMANAAVA
FAKANFRQRMMAATSSIGPGATNMLTSAALAHVGRLPLLLLPGDVFVSRLPDPVLQQVEDFEQGDVSANDCFRPVTRYFD
RITSPEQLLVALPRAIQVMTDPAQCGPVCLALPQDVQTFAYDWPEDFFAPPLIRMRRPPADALELADALDVLKAAKKPLI
VAGGGVLYSQAWDALRTFADTHGVPVAESQAGKGSLAWDHPLNLGAIGVTGSPAANRAAAQADVVFAVGTRLQDFTTGSH
ALYGDATLLSLNVQPFDAGKKRGRQLVADARTGLGQLSAALAGWRADPAWTAANRDQAAAWNARVTDLTTRIPKDTLPYD
AEVIGAVRDSAADAGRDSARDDLVVCAAGTLPAELHKLWRSGVPGNYHMDYAYSCMGYEVAGGLGAKLARPEREVIVIVG
DGSYMMLNAELATSVMLGRKIIVVILDNRGYGCIERLQLNCGGASFNNMLDDCVPEGGERSTIDFAMHARAMGAEAVHVR
DVGELRHEMKRARAATTSQVLVIDTTHQRTTDDGGAWWEVAVPQVSERAGVVDAHRAYLDAKTRQRR

Sequences:

>Translated_627_residues
MTTTVRLTVSQALVRYLAALRAEVVQPDGRTEILPYCGGVFAIFGHGNVAGLGEALHAEKDRLPTFRAHNEQGMANAAVA
FAKANFRQRMMAATSSIGPGATNMLTSAALAHVGRLPLLLLPGDVFVSRLPDPVLQQVEDFEQGDVSANDCFRPVTRYFD
RITSPEQLLVALPRAIQVMTDPAQCGPVCLALPQDVQTFAYDWPEDFFAPPLIRMRRPPADALELADALDVLKAAKKPLI
VAGGGVLYSQAWDALRTFADTHGVPVAESQAGKGSLAWDHPLNLGAIGVTGSPAANRAAAQADVVFAVGTRLQDFTTGSH
ALYGDATLLSLNVQPFDAGKKRGRQLVADARTGLGQLSAALAGWRADPAWTAANRDQAAAWNARVTDLTTRIPKDTLPYD
AEVIGAVRDSAADAGRDSARDDLVVCAAGTLPAELHKLWRSGVPGNYHMDYAYSCMGYEVAGGLGAKLARPEREVIVIVG
DGSYMMLNAELATSVMLGRKIIVVILDNRGYGCIERLQLNCGGASFNNMLDDCVPEGGERSTIDFAMHARAMGAEAVHVR
DVGELRHEMKRARAATTSQVLVIDTTHQRTTDDGGAWWEVAVPQVSERAGVVDAHRAYLDAKTRQRR
>Mature_626_residues
TTTVRLTVSQALVRYLAALRAEVVQPDGRTEILPYCGGVFAIFGHGNVAGLGEALHAEKDRLPTFRAHNEQGMANAAVAF
AKANFRQRMMAATSSIGPGATNMLTSAALAHVGRLPLLLLPGDVFVSRLPDPVLQQVEDFEQGDVSANDCFRPVTRYFDR
ITSPEQLLVALPRAIQVMTDPAQCGPVCLALPQDVQTFAYDWPEDFFAPPLIRMRRPPADALELADALDVLKAAKKPLIV
AGGGVLYSQAWDALRTFADTHGVPVAESQAGKGSLAWDHPLNLGAIGVTGSPAANRAAAQADVVFAVGTRLQDFTTGSHA
LYGDATLLSLNVQPFDAGKKRGRQLVADARTGLGQLSAALAGWRADPAWTAANRDQAAAWNARVTDLTTRIPKDTLPYDA
EVIGAVRDSAADAGRDSARDDLVVCAAGTLPAELHKLWRSGVPGNYHMDYAYSCMGYEVAGGLGAKLARPEREVIVIVGD
GSYMMLNAELATSVMLGRKIIVVILDNRGYGCIERLQLNCGGASFNNMLDDCVPEGGERSTIDFAMHARAMGAEAVHVRD
VGELRHEMKRARAATTSQVLVIDTTHQRTTDDGGAWWEVAVPQVSERAGVVDAHRAYLDAKTRQRR

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=28.4452296819788, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1786717, Length=514, Percent_Identity=25.6809338521401, Blast_Score=123, Evalue=4e-29,
Organism=Escherichia coli, GI1790104, Length=484, Percent_Identity=24.7933884297521, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1787096, Length=491, Percent_Identity=21.3849287169043, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1788716, Length=510, Percent_Identity=24.7058823529412, Blast_Score=79, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323755, Length=594, Percent_Identity=25.4208754208754, Blast_Score=142, Evalue=1e-34,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 67267; Mature: 67136

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTVRLTVSQALVRYLAALRAEVVQPDGRTEILPYCGGVFAIFGHGNVAGLGEALHAEK
CCEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHH
DRLPTFRAHNEQGMANAAVAFAKANFRQRMMAATSSIGPGATNMLTSAALAHVGRLPLLL
HCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEE
LPGDVFVSRLPDPVLQQVEDFEQGDVSANDCFRPVTRYFDRITSPEQLLVALPRAIQVMT
ECHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEC
DPAQCGPVCLALPQDVQTFAYDWPEDFFAPPLIRMRRPPADALELADALDVLKAAKKPLI
CCHHCCCEEEECCCCHHHHHCCCCHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCEE
VAGGGVLYSQAWDALRTFADTHGVPVAESQAGKGSLAWDHPLNLGAIGVTGSPAANRAAA
EECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHH
QADVVFAVGTRLQDFTTGSHALYGDATLLSLNVQPFDAGKKRGRQLVADARTGLGQLSAA
HHCEEEEECCCHHHCCCCCCEEECCEEEEEEECCCCCCHHHHCHHHHHHHHCCHHHHHHH
LAGWRADPAWTAANRDQAAAWNARVTDLTTRIPKDTLPYDAEVIGAVRDSAADAGRDSAR
HHCCCCCCCCCCCCCCCHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
DDLVVCAAGTLPAELHKLWRSGVPGNYHMDYAYSCMGYEVAGGLGAKLARPEREVIVIVG
CCEEEEECCCCHHHHHHHHHCCCCCCEEECEEEEECCEEECCCCCCCCCCCCCEEEEEEE
DGSYMMLNAELATSVMLGRKIIVVILDNRGYGCIERLQLNCGGASFNNMLDDCVPEGGER
CCCEEEEEHHHHHHHHHCCEEEEEEECCCCCCCEEHEEECCCCCCHHHHHHHHCCCCCCC
STIDFAMHARAMGAEAVHVRDVGELRHEMKRARAATTSQVLVIDTTHQRTTDDGGAWWEV
CEEHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEE
AVPQVSERAGVVDAHRAYLDAKTRQRR
ECCCHHHHCCCHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
TTTVRLTVSQALVRYLAALRAEVVQPDGRTEILPYCGGVFAIFGHGNVAGLGEALHAEK
CEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHH
DRLPTFRAHNEQGMANAAVAFAKANFRQRMMAATSSIGPGATNMLTSAALAHVGRLPLLL
HCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEE
LPGDVFVSRLPDPVLQQVEDFEQGDVSANDCFRPVTRYFDRITSPEQLLVALPRAIQVMT
ECHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEC
DPAQCGPVCLALPQDVQTFAYDWPEDFFAPPLIRMRRPPADALELADALDVLKAAKKPLI
CCHHCCCEEEECCCCHHHHHCCCCHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCEE
VAGGGVLYSQAWDALRTFADTHGVPVAESQAGKGSLAWDHPLNLGAIGVTGSPAANRAAA
EECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHH
QADVVFAVGTRLQDFTTGSHALYGDATLLSLNVQPFDAGKKRGRQLVADARTGLGQLSAA
HHCEEEEECCCHHHCCCCCCEEECCEEEEEEECCCCCCHHHHCHHHHHHHHCCHHHHHHH
LAGWRADPAWTAANRDQAAAWNARVTDLTTRIPKDTLPYDAEVIGAVRDSAADAGRDSAR
HHCCCCCCCCCCCCCCCHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
DDLVVCAAGTLPAELHKLWRSGVPGNYHMDYAYSCMGYEVAGGLGAKLARPEREVIVIVG
CCEEEEECCCCHHHHHHHHHCCCCCCEEECEEEEECCEEECCCCCCCCCCCCCEEEEEEE
DGSYMMLNAELATSVMLGRKIIVVILDNRGYGCIERLQLNCGGASFNNMLDDCVPEGGER
CCCEEEEEHHHHHHHHHCCEEEEEEECCCCCCCEEHEEECCCCCCHHHHHHHHCCCCCCC
STIDFAMHARAMGAEAVHVRDVGELRHEMKRARAATTSQVLVIDTTHQRTTDDGGAWWEV
CEEHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEE
AVPQVSERAGVVDAHRAYLDAKTRQRR
ECCCHHHHCCCHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; Decarboxylation [C]

Inhibitor: 2-oxobutanoate; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA