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The map label for this gene is purR [H]

Identifier: 116689257

GI number: 116689257

Start: 1365355

End: 1366380

Strand: Reverse

Name: purR [H]

Synonym: Bcen2424_1235

Alternate gene names: 116689257

Gene position: 1366380-1365355 (Counterclockwise)

Preceding gene: 116689258

Following gene: 116689256

Centisome position: 39.22

GC content: 70.08

Gene sequence:

>1026_bases
ATGACCTCGACCATCAAGGACGTCGCCGCCCTCGCGGGCTTCTCGATCGCCACCGTTTCGCGTGCGATCAACGCGCCGCA
TACCGTCAGCCCCGCCACGCTGCAGACGATCCGCACCGCCATCGACACGCTGCAGTTCCGCCCGAGCCCGCTCGGCCGGC
AACTGCGCGGCGAGCGCACGCGGCTCGTCGGCGTCGTCGTGCCGACGCTGTCGAACCCGGTGTTCGCCGACTGCCTGCAA
GGCATCGACGCGCTCGCGACCGCGGCCGGGTTCAAGCTCATACTGATGACGACCGAATACGATGCGGCGCGCGAACGTCA
TGCGATCGAGACGCTGCGCGAACAGCGCGTCGAAGGGCTGATCCTCACCGTCGCCGATGCGGATACGCACCCGCTGCTCG
ACATGCTCGATCGCGACGGCCCGCATTACGTGCTGATGCACAACGACACGCAGCGCCGCCCGTCGGTGTCGGTCGACAAC
CGCCGCGCCGCCTATGACGGCGTGCGGTTGCTGACCGCGCGCGGTCACCGTCGCGTGTTGATGCTGGCCGGTTCGCTCGC
CGCGTCGGATCGCGCGCGCCAGCGTCATCTCGGTTATGCGCAGGCACTCGACGAAAGCGGCGTGGCGCCGCTGCCGCCCG
TCGAAGTCGACTTCAACGCGCCCGAGCTGCCCGACGCGGTGCTCGCGCATCTCACCGCGCACGCCACGCGGCCGACGGCC
CTCTTCTGCAGCAACGACCTGCTCGCGATGGTCGTGATGCGCGGCCTGCGCCGCGCGGGTTTCTCGATTCCCGACGACCT
GTCGGTGCTCGGCTTCGACGGGATCGCGATCGGCGAGCTGCTCGCGCCGCCGCTCGCGAGCGTCACGACGCCGAACCGCG
ACATCGGCCGCCACGCGTGGCAGCGTCTCGTCGAATGTATCGGCGGTGCAGCGATCGACCGCACGTCGACGATCCTGCCG
CACGCGGTACGCGACGGCGCGACGATTGCACCGCCCGCCGCCGACCTGCAACTGCGCAAGGCCTGA

Upstream 100 bases:

>100_bases
GTGTCGTGCTTTTCGCGAAACAAGCGTCGCGCGCGTCATCGCGAAGTCACAAACGTTTTCGATAATTCCCCGGCCGAAAA
CGTTTACATCGCATGACTTC

Downstream 100 bases:

>100_bases
GCGCCGACCGCATGCGCCGCGCGGCCACACACACCCATAACCACACCCCAGCGCTACACCCACTCGCCCGGAGATTCGCC
GTGTCCTTTCGCCTGACCTC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MTSTIKDVAALAGFSIATVSRAINAPHTVSPATLQTIRTAIDTLQFRPSPLGRQLRGERTRLVGVVVPTLSNPVFADCLQ
GIDALATAAGFKLILMTTEYDAARERHAIETLREQRVEGLILTVADADTHPLLDMLDRDGPHYVLMHNDTQRRPSVSVDN
RRAAYDGVRLLTARGHRRVLMLAGSLAASDRARQRHLGYAQALDESGVAPLPPVEVDFNAPELPDAVLAHLTAHATRPTA
LFCSNDLLAMVVMRGLRRAGFSIPDDLSVLGFDGIAIGELLAPPLASVTTPNRDIGRHAWQRLVECIGGAAIDRTSTILP
HAVRDGATIAPPAADLQLRKA

Sequences:

>Translated_341_residues
MTSTIKDVAALAGFSIATVSRAINAPHTVSPATLQTIRTAIDTLQFRPSPLGRQLRGERTRLVGVVVPTLSNPVFADCLQ
GIDALATAAGFKLILMTTEYDAARERHAIETLREQRVEGLILTVADADTHPLLDMLDRDGPHYVLMHNDTQRRPSVSVDN
RRAAYDGVRLLTARGHRRVLMLAGSLAASDRARQRHLGYAQALDESGVAPLPPVEVDFNAPELPDAVLAHLTAHATRPTA
LFCSNDLLAMVVMRGLRRAGFSIPDDLSVLGFDGIAIGELLAPPLASVTTPNRDIGRHAWQRLVECIGGAAIDRTSTILP
HAVRDGATIAPPAADLQLRKA
>Mature_340_residues
TSTIKDVAALAGFSIATVSRAINAPHTVSPATLQTIRTAIDTLQFRPSPLGRQLRGERTRLVGVVVPTLSNPVFADCLQG
IDALATAAGFKLILMTTEYDAARERHAIETLREQRVEGLILTVADADTHPLLDMLDRDGPHYVLMHNDTQRRPSVSVDNR
RAAYDGVRLLTARGHRRVLMLAGSLAASDRARQRHLGYAQALDESGVAPLPPVEVDFNAPELPDAVLAHLTAHATRPTAL
FCSNDLLAMVVMRGLRRAGFSIPDDLSVLGFDGIAIGELLAPPLASVTTPNRDIGRHAWQRLVECIGGAAIDRTSTILPH
AVRDGATIAPPAADLQLRKA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=328, Percent_Identity=29.2682926829268, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1790194, Length=324, Percent_Identity=31.4814814814815, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1790715, Length=336, Percent_Identity=26.1904761904762, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI48994940, Length=337, Percent_Identity=28.1899109792285, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1789202, Length=297, Percent_Identity=31.6498316498317, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1788474, Length=333, Percent_Identity=29.7297297297297, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787948, Length=312, Percent_Identity=28.2051282051282, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1789068, Length=336, Percent_Identity=27.9761904761905, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1786540, Length=330, Percent_Identity=27.2727272727273, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1787580, Length=333, Percent_Identity=26.1261261261261, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787906, Length=331, Percent_Identity=25.3776435045317, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1790689, Length=309, Percent_Identity=22.9773462783171, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786268, Length=323, Percent_Identity=24.4582043343653, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36668; Mature: 36536

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTIKDVAALAGFSIATVSRAINAPHTVSPATLQTIRTAIDTLQFRPSPLGRQLRGERT
CCCHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCE
RLVGVVVPTLSNPVFADCLQGIDALATAAGFKLILMTTEYDAARERHAIETLREQRVEGL
EEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHCEE
ILTVADADTHPLLDMLDRDGPHYVLMHNDTQRRPSVSVDNRRAAYDGVRLLTARGHRRVL
EEEEECCCCCHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHCCEEEEECCCCCEEE
MLAGSLAASDRARQRHLGYAQALDESGVAPLPPVEVDFNAPELPDAVLAHLTAHATRPTA
EEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCE
LFCSNDLLAMVVMRGLRRAGFSIPDDLSVLGFDGIAIGELLAPPLASVTTPNRDIGRHAW
EEECCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHCCCHHHCCCCCHHHHHHHH
QRLVECIGGAAIDRTSTILPHAVRDGATIAPPAADLQLRKA
HHHHHHHCCCHHCCHHHHCCHHHCCCCEECCCCCCCEEECC
>Mature Secondary Structure 
TSTIKDVAALAGFSIATVSRAINAPHTVSPATLQTIRTAIDTLQFRPSPLGRQLRGERT
CCHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCE
RLVGVVVPTLSNPVFADCLQGIDALATAAGFKLILMTTEYDAARERHAIETLREQRVEGL
EEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHCEE
ILTVADADTHPLLDMLDRDGPHYVLMHNDTQRRPSVSVDNRRAAYDGVRLLTARGHRRVL
EEEEECCCCCHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHCCEEEEECCCCCEEE
MLAGSLAASDRARQRHLGYAQALDESGVAPLPPVEVDFNAPELPDAVLAHLTAHATRPTA
EEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCE
LFCSNDLLAMVVMRGLRRAGFSIPDDLSVLGFDGIAIGELLAPPLASVTTPNRDIGRHAW
EEECCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHCCCHHHCCCCCHHHHHHHH
QRLVECIGGAAIDRTSTILPHAVRDGATIAPPAADLQLRKA
HHHHHHHCCCHHCCHHHHCCHHHCCCCEECCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA