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The map label for this gene is potD [C]

Identifier: 116689256

GI number: 116689256

Start: 1364246

End: 1365343

Strand: Reverse

Name: potD [C]

Synonym: Bcen2424_1234

Alternate gene names: 116689256

Gene position: 1365343-1364246 (Counterclockwise)

Preceding gene: 116689257

Following gene: 116689255

Centisome position: 39.19

GC content: 66.12

Gene sequence:

>1098_bases
ATGCGCCGCGCGGCCACACACACCCATAACCACACCCCAGCGCTACACCCACTCGCCCGGAGATTCGCCGTGTCCTTTCG
CCTGACCTCGCTGCTGCGCGCGCTTGCCGCGCCGCTCGCCTTCGCCGCGCTCGTCGCCGGCGCCCCCGCGGCCCACGCCG
ACGAAACGGCGATCTGCTACAACTGCCCGCCCGAATGGGCCGACTGGGCCGCGCAGATCGCGGCGATCAAGCAGAAGACC
GGCATCCGCGTGCCGTTCGACAACAAGAACTCGGGCCAGTCGATCGCGCAGTTGATCGCCGAGCAGAAGAGCCCGGTGGC
CGACGTCGTCTACCTCGGCGTGTCGTCGGCGTTCCAGGCGAAGGACAAGGACGTGATCGCGCCGTACAAGCCCGCGCACT
GGAACGACATTCCCGCGAACCTGAAGGACCCGCAAGGCTACTGGTTCGCGATCCACTCGGGCACGCTCGGCTTCTTCGTG
AACAAGGACGCGCTCGACGGCAAGCCGGTGCCGCGTTCGTGGGCCGATCTGCTGAAGCCCGAATACAAGGGCATGGTCGG
CTATCTCGATCCGTCGAGCGCATTCGTCGGCTACGCGGGCGCGGTCGCCGTCAACCAGGCGCTCGGCGGCAGCCTCGACA
ACTTCAAGCCGGGCCTCGACTGGTTCCGCAAGCTGAAGGCGAACGCGCCGATCGTGCCGAAGCAGACCGCGTATGCGCGC
GTGCTGTCCGGCGAGATTCCGATCCTGCTCGACTACGACTTCGATGCGTATCGCGCGAAATACAAGGACAACGCGAACGT
CGAATTCGTGATCCCGAAGGAAGGCACGATCGCGGTGCCGTACGTGATGAGCCTCGTGAAGGGCGCGCCGCACGATGCGA
ACGGCAAGAAGGTGCTCGATTTCGTGCTGTCGGACGAAGGCCAGAAGCTGTGGGCCAACGCCTACCTGCGTCCGGTGCGC
GCGCAGGCGCTCGGCGCCGACATCGCCGCGAAGTTCCTGCCGGCGAGCGAATACGCGCGAGCGAAATCGGTCGACTTCGG
CAAGATGGCGGCCGGCCAGCAGGTGTTCGGCCAGCAGTACCTGCAAGTGATGCAGTAA

Upstream 100 bases:

>100_bases
ACCGCACGTCGACGATCCTGCCGCACGCGGTACGCGACGGCGCGACGATTGCACCGCCCGCCGCCGACCTGCAACTGCGC
AAGGCCTGAGCGCCGACCGC

Downstream 100 bases:

>100_bases
GCGGCCGGCCCCACGTCATGCTCGACCTGACTTTTCCGCTGCGCTGGCGCGTCGCGCTCGTCGCGCCGGCGCTCGCGGTA
TTCGCCGCGTTCTGGCTGCT

Product: extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MRRAATHTHNHTPALHPLARRFAVSFRLTSLLRALAAPLAFAALVAGAPAAHADETAICYNCPPEWADWAAQIAAIKQKT
GIRVPFDNKNSGQSIAQLIAEQKSPVADVVYLGVSSAFQAKDKDVIAPYKPAHWNDIPANLKDPQGYWFAIHSGTLGFFV
NKDALDGKPVPRSWADLLKPEYKGMVGYLDPSSAFVGYAGAVAVNQALGGSLDNFKPGLDWFRKLKANAPIVPKQTAYAR
VLSGEIPILLDYDFDAYRAKYKDNANVEFVIPKEGTIAVPYVMSLVKGAPHDANGKKVLDFVLSDEGQKLWANAYLRPVR
AQALGADIAAKFLPASEYARAKSVDFGKMAAGQQVFGQQYLQVMQ

Sequences:

>Translated_365_residues
MRRAATHTHNHTPALHPLARRFAVSFRLTSLLRALAAPLAFAALVAGAPAAHADETAICYNCPPEWADWAAQIAAIKQKT
GIRVPFDNKNSGQSIAQLIAEQKSPVADVVYLGVSSAFQAKDKDVIAPYKPAHWNDIPANLKDPQGYWFAIHSGTLGFFV
NKDALDGKPVPRSWADLLKPEYKGMVGYLDPSSAFVGYAGAVAVNQALGGSLDNFKPGLDWFRKLKANAPIVPKQTAYAR
VLSGEIPILLDYDFDAYRAKYKDNANVEFVIPKEGTIAVPYVMSLVKGAPHDANGKKVLDFVLSDEGQKLWANAYLRPVR
AQALGADIAAKFLPASEYARAKSVDFGKMAAGQQVFGQQYLQVMQ
>Mature_365_residues
MRRAATHTHNHTPALHPLARRFAVSFRLTSLLRALAAPLAFAALVAGAPAAHADETAICYNCPPEWADWAAQIAAIKQKT
GIRVPFDNKNSGQSIAQLIAEQKSPVADVVYLGVSSAFQAKDKDVIAPYKPAHWNDIPANLKDPQGYWFAIHSGTLGFFV
NKDALDGKPVPRSWADLLKPEYKGMVGYLDPSSAFVGYAGAVAVNQALGGSLDNFKPGLDWFRKLKANAPIVPKQTAYAR
VLSGEIPILLDYDFDAYRAKYKDNANVEFVIPKEGTIAVPYVMSLVKGAPHDANGKKVLDFVLSDEGQKLWANAYLRPVR
AQALGADIAAKFLPASEYARAKSVDFGKMAAGQQVFGQQYLQVMQ

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine And Spermidine. Polyamine Binding Protein. [C]

COG id: COG1840

COG function: function code P; ABC-type Fe3+ transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39669; Mature: 39669

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRAATHTHNHTPALHPLARRFAVSFRLTSLLRALAAPLAFAALVAGAPAAHADETAICY
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
NCPPEWADWAAQIAAIKQKTGIRVPFDNKNSGQSIAQLIAEQKSPVADVVYLGVSSAFQA
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHEEEECCCHHHHC
KDKDVIAPYKPAHWNDIPANLKDPQGYWFAIHSGTLGFFVNKDALDGKPVPRSWADLLKP
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHCH
EYKGMVGYLDPSSAFVGYAGAVAVNQALGGSLDNFKPGLDWFRKLKANAPIVPKQTAYAR
HHCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHH
VLSGEIPILLDYDFDAYRAKYKDNANVEFVIPKEGTIAVPYVMSLVKGAPHDANGKKVLD
HHCCCCCEEEECCCHHHHHHCCCCCCEEEEECCCCCEEHHHHHHHHHCCCCCCCCCEEEH
FVLSDEGQKLWANAYLRPVRAQALGADIAAKFLPASEYARAKSVDFGKMAAGQQVFGQQY
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHCCHHHHHHHH
LQVMQ
HHHHC
>Mature Secondary Structure
MRRAATHTHNHTPALHPLARRFAVSFRLTSLLRALAAPLAFAALVAGAPAAHADETAICY
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
NCPPEWADWAAQIAAIKQKTGIRVPFDNKNSGQSIAQLIAEQKSPVADVVYLGVSSAFQA
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHEEEECCCHHHHC
KDKDVIAPYKPAHWNDIPANLKDPQGYWFAIHSGTLGFFVNKDALDGKPVPRSWADLLKP
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHCH
EYKGMVGYLDPSSAFVGYAGAVAVNQALGGSLDNFKPGLDWFRKLKANAPIVPKQTAYAR
HHCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHH
VLSGEIPILLDYDFDAYRAKYKDNANVEFVIPKEGTIAVPYVMSLVKGAPHDANGKKVLD
HHCCCCCEEEECCCHHHHHHCCCCCCEEEEECCCCCEEHHHHHHHHHCCCCCCCCCEEEH
FVLSDEGQKLWANAYLRPVRAQALGADIAAKFLPASEYARAKSVDFGKMAAGQQVFGQQY
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHCCHHHHHHHH
LQVMQ
HHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]