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The map label for this gene is yagF [H]

Identifier: 116624435

GI number: 116624435

Start: 6718659

End: 6720605

Strand: Reverse

Name: yagF [H]

Synonym: Acid_5357

Alternate gene names: 116624435

Gene position: 6720605-6718659 (Counterclockwise)

Preceding gene: 116624437

Following gene: 116624434

Centisome position: 67.44

GC content: 68.67

Gene sequence:

>1947_bases
ATGGATAGTGGGCTCCAACACATTCTCGATTCGGGTGACCGCTCGCTCTTCGACGTGCGGTCCAAGGCCGCCGGTCCCCA
GGGCGCGGCGCCGATCACGCCGGAGATGCTGCTGCTGCGGCCCTCCGGCGACCTTTTCGGCTGGACGCAGAACGCCGGCA
TGGGATGGGATCCGGCGCTGCTCGGCGGCAAGGAATTCCTGATTCTCAGCACCCATGGCGGCATTCGCGCCGCCGATGGC
ACCCCCGTCGCGCTCGGCTACCATACCGGCCACTGGGAGGTCGCGCTGCTGATGGACGCCGCCGCGAAGGAGTTGAAGTC
GCGCGGCGCGATCCCATTCGCGGCCTACTGCACGGACCCCTGCGACGGCCGCACTCAGGGCACCACCGGGATGTTCGATT
CCCTGCCCTACCGCAACGATGCCGCCGGCATTTTCCGCCGGCTGATCCGCTCCCTGCCCACGCGCAGCGGCGTGCTTGGC
GTCGCCACCTGCGACAAGGGGCTGCCGGCGATGATGATGGCGCTGGCCGGGACGCGCGACCTTCCCGCGGTGCTCATCCC
CGGCGGCGTCACGCTGCTGCCTGAGCAAGGCGAGGACGCCGGCAAAGTGCAATCGGTCGGCGCGCGGTTCTCGCACGGGC
AGATCACGCTCGAAGAGGCGGCGGAGGCGATGTGCCGCTCCTGTGCCAGCCCCGGCGGAGGCTGCCAGTTTCTGGGCACG
GCGGCTACCGCCCAGGTAGTCGGCGAGGCGCTGGGCATGTCGCTGCCGCATTCGGCGCTCGCGCCTTCCGGGCACCCCAT
CTGGGTTGACATGGCGCGCCGCTCGGCACGCGCGGCGCTCTCGCTGGAGTCCCGCGGGCTGACGATGAGCCACGTGCTCT
CCGCGGCCAGCGTGCGCAATGCGATGGTGACCCACGCGGCCTTCGGCGGCTCGACCAATCTGATTCTGCACCTGCCCGCG
ATCGCCTTCTCGGCCGGCCTAACGCGCCCCACGGTGGACGATTGGACCCACGTCAACCGGCAGGTGCCGCGCCTTGTCGA
TGCACTGCCCAACGGTCCGAAGATGCACCCCACCGTGCAGGTCTTTCTGGCGGGCGGCGTTCCCGAAGTGATGCTGCACT
TGCGGCGCGCCGGCCTGCTCGATACCGCGGCGCTGACCGTCACCGGCGAATCGCTGGGTGCGATGCTCGATTGGTGGGAG
CAATCCGAACGCCGCGCTTCCTTGCGCCGCACGCTTCGCGAACGCGACGGCGTAGACCCCGACGACGTGATCATGGACCC
CGCGGGGGCGCATCGCCGCGGACTCACATCGACCGTGACCTTTCCCAAGGGCAATCTCGCTCCCGGCGGCGCGGTGATCA
AGAGCACGTCTATCGACCCAAGCGTAGTCGGTGAAGATGGGGTCTACCGCATGACCGGTCCGGCGCGCGTCTTCCGCAGC
GAACACTCCGCCGTTGCCGCAATCAAGAGCGGCGAGATTCACGCGGGCGATGTTTTGGTGCTGATGTGTCGCGGGCCGCT
CGGTTCCGGCATGGAGGAGATCTATCAGATCACCTCGGCCCTGAAGTACCTGCCGTTCGGGAAACACGTCTCCGTGATCA
CCGACGCGCGCTTCAGCGGCGTTTCCACGGGCGCGTGCATCGGGCACGTGACGCCCGAGGCCTTGGCCGGCGGACCGCTC
GGCAAAGTGCGCGAAGGCGACCAGATCGAAATCGTGATCGACCGCGTGAAACTGGAAGGCACCGTCAACCTGGTGGTCGA
TGGCGACGCGGACGAAGGCGCGCGCGTCCTAGCCGCGCGACCATTGCGCGACGATCTCGCGCCCGACCCCGCGCTGCCGC
CGGAGACGCGCTTGTGGGCGATGCTGCAGGATGCGAGCGGGGGCACCTGGGCCGGGTGCGTGTTCGATGTCGAAGCCATC
GGCTCCAGGTTGAATCCCCGACGTTGA

Upstream 100 bases:

>100_bases
CGAAATCCGCGTGCGCGCCCTTCATGTGAAGTAGATCGAGCAGATGTTTTCGAAGCGGGTCTGTCTGTGCCATGCCTCCA
GTTTGCTACGATCAGACCGC

Downstream 100 bases:

>100_bases
AACAACGATAGCCGCATAATGCGAGCGCCGGTCGCCGAGTACAAAATACTTAGTCCGGTACTTCGGGGCGGGAAGGCACA
TTCCCATACCGCCGCAAAGC

Product: hypothetical protein

Products: 3-methyl-2-oxobutanoate; H2O

Alternate protein names: NA

Number of amino acids: Translated: 648; Mature: 648

Protein sequence:

>648_residues
MDSGLQHILDSGDRSLFDVRSKAAGPQGAAPITPEMLLLRPSGDLFGWTQNAGMGWDPALLGGKEFLILSTHGGIRAADG
TPVALGYHTGHWEVALLMDAAAKELKSRGAIPFAAYCTDPCDGRTQGTTGMFDSLPYRNDAAGIFRRLIRSLPTRSGVLG
VATCDKGLPAMMMALAGTRDLPAVLIPGGVTLLPEQGEDAGKVQSVGARFSHGQITLEEAAEAMCRSCASPGGGCQFLGT
AATAQVVGEALGMSLPHSALAPSGHPIWVDMARRSARAALSLESRGLTMSHVLSAASVRNAMVTHAAFGGSTNLILHLPA
IAFSAGLTRPTVDDWTHVNRQVPRLVDALPNGPKMHPTVQVFLAGGVPEVMLHLRRAGLLDTAALTVTGESLGAMLDWWE
QSERRASLRRTLRERDGVDPDDVIMDPAGAHRRGLTSTVTFPKGNLAPGGAVIKSTSIDPSVVGEDGVYRMTGPARVFRS
EHSAVAAIKSGEIHAGDVLVLMCRGPLGSGMEEIYQITSALKYLPFGKHVSVITDARFSGVSTGACIGHVTPEALAGGPL
GKVREGDQIEIVIDRVKLEGTVNLVVDGDADEGARVLAARPLRDDLAPDPALPPETRLWAMLQDASGGTWAGCVFDVEAI
GSRLNPRR

Sequences:

>Translated_648_residues
MDSGLQHILDSGDRSLFDVRSKAAGPQGAAPITPEMLLLRPSGDLFGWTQNAGMGWDPALLGGKEFLILSTHGGIRAADG
TPVALGYHTGHWEVALLMDAAAKELKSRGAIPFAAYCTDPCDGRTQGTTGMFDSLPYRNDAAGIFRRLIRSLPTRSGVLG
VATCDKGLPAMMMALAGTRDLPAVLIPGGVTLLPEQGEDAGKVQSVGARFSHGQITLEEAAEAMCRSCASPGGGCQFLGT
AATAQVVGEALGMSLPHSALAPSGHPIWVDMARRSARAALSLESRGLTMSHVLSAASVRNAMVTHAAFGGSTNLILHLPA
IAFSAGLTRPTVDDWTHVNRQVPRLVDALPNGPKMHPTVQVFLAGGVPEVMLHLRRAGLLDTAALTVTGESLGAMLDWWE
QSERRASLRRTLRERDGVDPDDVIMDPAGAHRRGLTSTVTFPKGNLAPGGAVIKSTSIDPSVVGEDGVYRMTGPARVFRS
EHSAVAAIKSGEIHAGDVLVLMCRGPLGSGMEEIYQITSALKYLPFGKHVSVITDARFSGVSTGACIGHVTPEALAGGPL
GKVREGDQIEIVIDRVKLEGTVNLVVDGDADEGARVLAARPLRDDLAPDPALPPETRLWAMLQDASGGTWAGCVFDVEAI
GSRLNPRR
>Mature_648_residues
MDSGLQHILDSGDRSLFDVRSKAAGPQGAAPITPEMLLLRPSGDLFGWTQNAGMGWDPALLGGKEFLILSTHGGIRAADG
TPVALGYHTGHWEVALLMDAAAKELKSRGAIPFAAYCTDPCDGRTQGTTGMFDSLPYRNDAAGIFRRLIRSLPTRSGVLG
VATCDKGLPAMMMALAGTRDLPAVLIPGGVTLLPEQGEDAGKVQSVGARFSHGQITLEEAAEAMCRSCASPGGGCQFLGT
AATAQVVGEALGMSLPHSALAPSGHPIWVDMARRSARAALSLESRGLTMSHVLSAASVRNAMVTHAAFGGSTNLILHLPA
IAFSAGLTRPTVDDWTHVNRQVPRLVDALPNGPKMHPTVQVFLAGGVPEVMLHLRRAGLLDTAALTVTGESLGAMLDWWE
QSERRASLRRTLRERDGVDPDDVIMDPAGAHRRGLTSTVTFPKGNLAPGGAVIKSTSIDPSVVGEDGVYRMTGPARVFRS
EHSAVAAIKSGEIHAGDVLVLMCRGPLGSGMEEIYQITSALKYLPFGKHVSVITDARFSGVSTGACIGHVTPEALAGGPL
GKVREGDQIEIVIDRVKLEGTVNLVVDGDADEGARVLAARPLRDDLAPDPALPPETRLWAMLQDASGGTWAGCVFDVEAI
GSRLNPRR

Specific function: Unknown

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI1786464, Length=645, Percent_Identity=63.4108527131783, Blast_Score=841, Evalue=0.0,
Organism=Escherichia coli, GI2367371, Length=646, Percent_Identity=59.1331269349845, Blast_Score=788, Evalue=0.0,
Organism=Escherichia coli, GI48994964, Length=566, Percent_Identity=29.6819787985866, Blast_Score=179, Evalue=6e-46,
Organism=Escherichia coli, GI1788157, Length=471, Percent_Identity=30.3609341825902, Blast_Score=148, Evalue=9e-37,
Organism=Saccharomyces cerevisiae, GI6322476, Length=472, Percent_Identity=31.7796610169492, Blast_Score=182, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR017798
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: 4.2.1.9

Molecular weight: Translated: 68072; Mature: 68072

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00886 ILVD_EDD_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSGLQHILDSGDRSLFDVRSKAAGPQGAAPITPEMLLLRPSGDLFGWTQNAGMGWDPAL
CCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCEECCCCCCCCHHH
LGGKEFLILSTHGGIRAADGTPVALGYHTGHWEVALLMDAAAKELKSRGAIPFAAYCTDP
CCCCEEEEEECCCCEEECCCCEEEEEEECCCEEEEEHHHHHHHHHHHCCCCCEEEEECCC
CDGRTQGTTGMFDSLPYRNDAAGIFRRLIRSLPTRSGVLGVATCDKGLPAMMMALAGTRD
CCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCC
LPAVLIPGGVTLLPEQGEDAGKVQSVGARFSHGQITLEEAAEAMCRSCASPGGGCQFLGT
CCEEEECCCEEECCCCCCCCHHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCCCEEEECH
AATAQVVGEALGMSLPHSALAPSGHPIWVDMARRSARAALSLESRGLTMSHVLSAASVRN
HHHHHHHHHHHCCCCCCHHCCCCCCEEEEEHHHHHHHHEEEHHCCCCCHHHHHHHHHHHH
AMVTHAAFGGSTNLILHLPAIAFSAGLTRPTVDDWTHVNRQVPRLVDALPNGPKMHPTVQ
HHHHHHCCCCCCEEEEEECHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEE
VFLAGGVPEVMLHLRRAGLLDTAALTVTGESLGAMLDWWEQSERRASLRRTLRERDGVDP
EEEECCHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
DDVIMDPAGAHRRGLTSTVTFPKGNLAPGGAVIKSTSIDPSVVGEDGVYRMTGPARVFRS
HHEEECCCCCCCCCCCEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCEEECCHHHHHHC
EHSAVAAIKSGEIHAGDVLVLMCRGPLGSGMEEIYQITSALKYLPFGKHVSVITDARFSG
CCCEEEEEECCCEECCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
VSTGACIGHVTPEALAGGPLGKVREGDQIEIVIDRVKLEGTVNLVVDGDADEGARVLAAR
CCCCCEEECCCHHHHCCCCCCCCCCCCEEEEEEEEEEECEEEEEEEECCCCCCCEEEEEC
PLRDDLAPDPALPPETRLWAMLQDASGGTWAGCVFDVEAIGSRLNPRR
CCHHCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEEHHHHHCCCCCCC
>Mature Secondary Structure
MDSGLQHILDSGDRSLFDVRSKAAGPQGAAPITPEMLLLRPSGDLFGWTQNAGMGWDPAL
CCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCEECCCCCCCCHHH
LGGKEFLILSTHGGIRAADGTPVALGYHTGHWEVALLMDAAAKELKSRGAIPFAAYCTDP
CCCCEEEEEECCCCEEECCCCEEEEEEECCCEEEEEHHHHHHHHHHHCCCCCEEEEECCC
CDGRTQGTTGMFDSLPYRNDAAGIFRRLIRSLPTRSGVLGVATCDKGLPAMMMALAGTRD
CCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCC
LPAVLIPGGVTLLPEQGEDAGKVQSVGARFSHGQITLEEAAEAMCRSCASPGGGCQFLGT
CCEEEECCCEEECCCCCCCCHHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCCCEEEECH
AATAQVVGEALGMSLPHSALAPSGHPIWVDMARRSARAALSLESRGLTMSHVLSAASVRN
HHHHHHHHHHHCCCCCCHHCCCCCCEEEEEHHHHHHHHEEEHHCCCCCHHHHHHHHHHHH
AMVTHAAFGGSTNLILHLPAIAFSAGLTRPTVDDWTHVNRQVPRLVDALPNGPKMHPTVQ
HHHHHHCCCCCCEEEEEECHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEE
VFLAGGVPEVMLHLRRAGLLDTAALTVTGESLGAMLDWWEQSERRASLRRTLRERDGVDP
EEEECCHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
DDVIMDPAGAHRRGLTSTVTFPKGNLAPGGAVIKSTSIDPSVVGEDGVYRMTGPARVFRS
HHEEECCCCCCCCCCCEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCEEECCHHHHHHC
EHSAVAAIKSGEIHAGDVLVLMCRGPLGSGMEEIYQITSALKYLPFGKHVSVITDARFSG
CCCEEEEEECCCEECCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
VSTGACIGHVTPEALAGGPLGKVREGDQIEIVIDRVKLEGTVNLVVDGDADEGARVLAAR
CCCCCEEECCCHHHHCCCCCCCCCCCCEEEEEEEEEEECEEEEEEEECCCCCCCEEEEEC
PLRDDLAPDPALPPETRLWAMLQDASGGTWAGCVFDVEAIGSRLNPRR
CCHHCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEEHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,3-dihydroxy-3-methylbutanoate

Specific reaction: 2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]