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The map label for this gene is hemD [H]

Identifier: 116622287

GI number: 116622287

Start: 4030589

End: 4032073

Strand: Direct

Name: hemD [H]

Synonym: Acid_3181

Alternate gene names: 116622287

Gene position: 4030589-4032073 (Clockwise)

Preceding gene: 116622286

Following gene: 116622288

Centisome position: 40.44

GC content: 68.48

Gene sequence:

>1485_bases
ATGAAGGGCAAGGTGTATCTGGTGGGGGCGGGGCCGGGGGATCCGGAACTGATTACGGTGAAGGGGCGAAGGGTCCTGCA
AGGGGCGGATGCGGTGCTGTACGATCACCTGGCGGCCGCGGCCCTGCTCGACCTCGCCCCCGCTCACGCAGAGCGGGTCT
ACGTCGGCAAGAAGAAGTCGGCGCACGAGTTCACGCAGGAAGAGATTTGCGCCATGCTGATCGAACGCGGGCGGCGCGGG
CTGACAGTGGTGCGGCTGAAGGGTGGCGACCCGTTTATTTTCGGGCGCGGCGGGGAAGAGGCGGAGGCGCTGGCGGATGC
GGGGATCGCGTTCGAAGTGGTGCCGGGGGTGACCACGCCGCTGGGCATCGCAGCCTACACCGGCGTGCCGCTGACACATC
GCGAACACACCTCGGCAGTGACGTTCGTGACCGGGCACGCGGTGCAGGCGATCGATTGGGACAAAGTGGGACACGCCGAG
ACGCTGGTGATCTTCATGGGGCTGACCACGTTCCCAGAGATCGCGCGTGAATTGATCGCGCGGGGCCGGCCGGCGGAGAC
TCCAGCGATGGCGGTGCGTTGGGCCACGCGGGCAGACCAGGAGACGGTGGTCGGCACGCTGGGAACGCTGACCGGAATGC
TGGCCGGGATGAAGCCGCCGGCAACCATCGTGGTGGGCGAGGTGGTGCGGCTGCGCGAGAAGCTGGATTGGTACGGACGG
CTGCCGCTTGCGGGAAAGCGGATCGTGGTGACGCGGGCGCGGGAACAGGCGGACGCGCTGGGCGCCGCGTTGAGCTCGCT
GGGTGCGGGCGTGATCGAGCTGCCGACGATCGAGATCGGTCCGGCGGCGGATAGCGGCCCGCTGGAGCGCGCGATCGCGG
AGTTGGAAACCTACGACTGGCTGATCTTCACGAGCGCCAACGGAGTGCGGTTTTTCGTGGAGCGGCTCGACGATCTGCGC
AAGCTGCGCTCGAAGATCTGCGCGATCGGCCCGGCGACGCGGGCGGCGGTGGAAGCGCTCCACGTGAAGGTGGACCTGAT
GGGCAAGGAATACGTCGCGGAGGGGCTGCTCGAAGCCTTCGCGGCGCACGACCTGGCGGGCAAGCGGGTGCTGCTGCCGC
GGGCGGCGGTGGCGCGCGACCTGGTGCCGGTGGAACTGGCGAAGCGCGGCGCGCAGGTGGATGTGGTGGAAGCGTACCGG
ACGGCGGCTCCGGCGGGGCTGGGGGCGCGGGTGCGGGAAGTCTTCGCGCGGCGGCCGGATTGCGTCACGTTCACGAGCTC
TTCCACGGTCCGGAATTTCGTGGAGGCGGCCGGAGCAGGGGCGCTCGAAGGCGTACGGGTGGCGTCTATCGGGCCGGTCA
CGTCGGCCACGGCCCGGGAACTCGGAGTGGCGGTAACGGTGGAAGCGCGGCCATTTACGGTGGATGGACTTGTCGATGCA
GTGCTCGAGTTGTACACTGAAATGACTCCCCCGTCAACTGAATGA

Upstream 100 bases:

>100_bases
GTGCAGGGAGAAGTGTGTTTTCCCTTGACAAGTACTTATCATGGATGCCCCACAAGCCCAATGGATGTTCGGCGGGAAAG
GGCAGGAGGCCTTTCCCACA

Downstream 100 bases:

>100_bases
GTGCACACATTATTGGTGTAGCGGGTCCGTCCAGCTCCGGGAAATCCGAGCTGGCGCGGCAACTGGCAGAGAGATTGCCC
GGGACCTCCATCGTTTCCCT

Product: uroporphyrinogen-III C-methyltransferase/uroporphyrinogen-III synthase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Uroporphyrinogen-III synthase; UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MKGKVYLVGAGPGDPELITVKGRRVLQGADAVLYDHLAAAALLDLAPAHAERVYVGKKKSAHEFTQEEICAMLIERGRRG
LTVVRLKGGDPFIFGRGGEEAEALADAGIAFEVVPGVTTPLGIAAYTGVPLTHREHTSAVTFVTGHAVQAIDWDKVGHAE
TLVIFMGLTTFPEIARELIARGRPAETPAMAVRWATRADQETVVGTLGTLTGMLAGMKPPATIVVGEVVRLREKLDWYGR
LPLAGKRIVVTRAREQADALGAALSSLGAGVIELPTIEIGPAADSGPLERAIAELETYDWLIFTSANGVRFFVERLDDLR
KLRSKICAIGPATRAAVEALHVKVDLMGKEYVAEGLLEAFAAHDLAGKRVLLPRAAVARDLVPVELAKRGAQVDVVEAYR
TAAPAGLGARVREVFARRPDCVTFTSSSTVRNFVEAAGAGALEGVRVASIGPVTSATARELGVAVTVEARPFTVDGLVDA
VLELYTEMTPPSTE

Sequences:

>Translated_494_residues
MKGKVYLVGAGPGDPELITVKGRRVLQGADAVLYDHLAAAALLDLAPAHAERVYVGKKKSAHEFTQEEICAMLIERGRRG
LTVVRLKGGDPFIFGRGGEEAEALADAGIAFEVVPGVTTPLGIAAYTGVPLTHREHTSAVTFVTGHAVQAIDWDKVGHAE
TLVIFMGLTTFPEIARELIARGRPAETPAMAVRWATRADQETVVGTLGTLTGMLAGMKPPATIVVGEVVRLREKLDWYGR
LPLAGKRIVVTRAREQADALGAALSSLGAGVIELPTIEIGPAADSGPLERAIAELETYDWLIFTSANGVRFFVERLDDLR
KLRSKICAIGPATRAAVEALHVKVDLMGKEYVAEGLLEAFAAHDLAGKRVLLPRAAVARDLVPVELAKRGAQVDVVEAYR
TAAPAGLGARVREVFARRPDCVTFTSSSTVRNFVEAAGAGALEGVRVASIGPVTSATARELGVAVTVEARPFTVDGLVDA
VLELYTEMTPPSTE
>Mature_494_residues
MKGKVYLVGAGPGDPELITVKGRRVLQGADAVLYDHLAAAALLDLAPAHAERVYVGKKKSAHEFTQEEICAMLIERGRRG
LTVVRLKGGDPFIFGRGGEEAEALADAGIAFEVVPGVTTPLGIAAYTGVPLTHREHTSAVTFVTGHAVQAIDWDKVGHAE
TLVIFMGLTTFPEIARELIARGRPAETPAMAVRWATRADQETVVGTLGTLTGMLAGMKPPATIVVGEVVRLREKLDWYGR
LPLAGKRIVVTRAREQADALGAALSSLGAGVIELPTIEIGPAADSGPLERAIAELETYDWLIFTSANGVRFFVERLDDLR
KLRSKICAIGPATRAAVEALHVKVDLMGKEYVAEGLLEAFAAHDLAGKRVLLPRAAVARDLVPVELAKRGAQVDVVEAYR
TAAPAGLGARVREVFARRPDCVTFTSSSTVRNFVEAAGAGALEGVRVASIGPVTSATARELGVAVTVEARPFTVDGLVDA
VLELYTEMTPPSTE

Specific function: May catalyze sequential reactions to synthesize uroporphyrinogen III from hydroxymethylbilane (HMB) and then precorrin-2, which are intermediate compounds in both vitamin B12 and siroheme biosyntheses [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=240, Percent_Identity=45.8333333333333, Blast_Score=220, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6322922, Length=234, Percent_Identity=32.9059829059829, Blast_Score=110, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =4.2.1.75 [H]

Molecular weight: Translated: 52397; Mature: 52397

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGKVYLVGAGPGDPELITVKGRRVLQGADAVLYDHLAAAALLDLAPAHAERVYVGKKKS
CCCEEEEEECCCCCCCEEEECCCEEECCCHHHHHHHHHHHHHHHHCCCHHCEEEECCCCC
AHEFTQEEICAMLIERGRRGLTVVRLKGGDPFIFGRGGEEAEALADAGIAFEVVPGVTTP
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEECCCCCHHHHHHHCCCEEEECCCCCCC
LGIAAYTGVPLTHREHTSAVTFVTGHAVQAIDWDKVGHAETLVIFMGLTTFPEIARELIA
CCHHHHCCCCCCCCCCCCEEEEEECCEEEEECCCCCCCCEEEEEEECHHHHHHHHHHHHH
RGRPAETPAMAVRWATRADQETVVGTLGTLTGMLAGMKPPATIVVGEVVRLREKLDWYGR
CCCCCCCCHHEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
LPLAGKRIVVTRAREQADALGAALSSLGAGVIELPTIEIGPAADSGPLERAIAELETYDW
CCCCCCEEEEEECHHHHHHHHHHHHHHCCCEEECCEEEECCCCCCCHHHHHHHHHHHCCE
LIFTSANGVRFFVERLDDLRKLRSKICAIGPATRAAVEALHVKVDLMGKEYVAEGLLEAF
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEEEECCHHHHHHHHHHHH
AAHDLAGKRVLLPRAAVARDLVPVELAKRGAQVDVVEAYRTAAPAGLGARVREVFARRPD
HHHHCCCCEEECCHHHHHHCCCHHHHHHCCCCEEHHHHHHHHCCCCCCHHHHHHHHCCCC
CVTFTSSSTVRNFVEAAGAGALEGVRVASIGPVTSATARELGVAVTVEARPFTVDGLVDA
EEEECCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHCCEEEEEECCCCCHHHHHHH
VLELYTEMTPPSTE
HHHHHHHCCCCCCC
>Mature Secondary Structure
MKGKVYLVGAGPGDPELITVKGRRVLQGADAVLYDHLAAAALLDLAPAHAERVYVGKKKS
CCCEEEEEECCCCCCCEEEECCCEEECCCHHHHHHHHHHHHHHHHCCCHHCEEEECCCCC
AHEFTQEEICAMLIERGRRGLTVVRLKGGDPFIFGRGGEEAEALADAGIAFEVVPGVTTP
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEECCCCCHHHHHHHCCCEEEECCCCCCC
LGIAAYTGVPLTHREHTSAVTFVTGHAVQAIDWDKVGHAETLVIFMGLTTFPEIARELIA
CCHHHHCCCCCCCCCCCCEEEEEECCEEEEECCCCCCCCEEEEEEECHHHHHHHHHHHHH
RGRPAETPAMAVRWATRADQETVVGTLGTLTGMLAGMKPPATIVVGEVVRLREKLDWYGR
CCCCCCCCHHEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
LPLAGKRIVVTRAREQADALGAALSSLGAGVIELPTIEIGPAADSGPLERAIAELETYDW
CCCCCCEEEEEECHHHHHHHHHHHHHHCCCEEECCEEEECCCCCCCHHHHHHHHHHHCCE
LIFTSANGVRFFVERLDDLRKLRSKICAIGPATRAAVEALHVKVDLMGKEYVAEGLLEAF
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEEEECCHHHHHHHHHHHH
AAHDLAGKRVLLPRAAVARDLVPVELAKRGAQVDVVEAYRTAAPAGLGARVREVFARRPD
HHHHCCCCEEECCHHHHHHCCCHHHHHHCCCCEEHHHHHHHHCCCCCCHHHHHHHHCCCC
CVTFTSSSTVRNFVEAAGAGALEGVRVASIGPVTSATARELGVAVTVEARPFTVDGLVDA
EEEECCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHCCEEEEEECCCCCHHHHHHH
VLELYTEMTPPSTE
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7665501 [H]