Click here to switch to the map view.

The map label for this gene is iolB [H]

Identifier: 116621853

GI number: 116621853

Start: 3473478

End: 3474314

Strand: Reverse

Name: iolB [H]

Synonym: Acid_2736

Alternate gene names: 116621853

Gene position: 3474314-3473478 (Counterclockwise)

Preceding gene: 116621861

Following gene: 116621852

Centisome position: 34.86

GC content: 65.23

Gene sequence:

>837_bases
ATGAGCAGCCTGGCCCCGGCGATTAACGCCCAGACCTGCGTAATCCGTGCCACTCACAAGACTAAGGGACGAAGCCGGTC
GCTGGCCCCGGCAACTGCCGCGGTGCGACACCTTCACTACGGACGGATCATTCTGGACGCGGCCGATGCGCCGCTCGAGT
TTTCCACCGGTACACATGAGACCGGCCTGATCGCGCTCAACGGCCATGCCACCATCCGGCTGGGCGCGGAGAGCTTCGCG
CTCGGACGCTACGACGCGATCTACATTCCGCGCGACCGTGCGGTGGTGGTCACGCCCGGCGACTCGGGCTGCGACCTGGC
GGAGATCTCGGCGCCGGTGACGGGCGACTACCCGGTGCAGTATGTTCCCTTCTCCCAGGTGCAGAGCGACCCGAACCTGC
ACTTCGCGGCGGGCGCGCCTTCGTGCGAGCGCGATTTGAACATCGTCTTCGGCAAGAATGTGCAGGCCGGCCGGATCATG
GCGGGCGTGACGTTCAGCAAGCCGGGCAACTGGACCTCCTGGCCGCCGCACGAACACGGCCGCATGCTCGAGGAAGCCTA
CCTCTATATCGATATGCCGGCGCCCGGATGGGGCCTGCAACTGGTGTTCACGAATTCCAACGAGCCGGAACTGGTGGCCA
AGGTCGGCGAAGGCGACGTGGTGGTGATGCCGGAAGGCTATCACCCGAATGTGGCGGCGCCGGGCAATTCGATCAACTTC
CTGTGGATGATGGCGGCGCACACCGAAGTGGCCGACCGTCAATTCGGAGTGGTCAACGTACATCCAGCGTACGCGGCCGG
CGGCAGCGGCCTGGAAGCCGCGCACGAGACAAAATGA

Upstream 100 bases:

>100_bases
GAATATGCCGAAGATCTCATTGACTCGCTGGTGAAATGCACCTATTCTATGGTACGGAATCTGATCTATATCATTTCACG
ATGTGAAACAGGGAGAGCAA

Downstream 100 bases:

>100_bases
AACAGGGCTGGCAGGTAGTGGCGGCTCTGGCCTGGATGGCGGCAGGGCTGCAAGCGGCGTCGCACATCAAGACGCTGAAA
CTCGCGGTCACCAACCCCAC

Product: hypothetical protein

Products: 3-Deoxy-D-Glycero-2,5-Hexodiulosonate. [C]

Alternate protein names: 5DG isomerase [H]

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MSSLAPAINAQTCVIRATHKTKGRSRSLAPATAAVRHLHYGRIILDAADAPLEFSTGTHETGLIALNGHATIRLGAESFA
LGRYDAIYIPRDRAVVVTPGDSGCDLAEISAPVTGDYPVQYVPFSQVQSDPNLHFAAGAPSCERDLNIVFGKNVQAGRIM
AGVTFSKPGNWTSWPPHEHGRMLEEAYLYIDMPAPGWGLQLVFTNSNEPELVAKVGEGDVVVMPEGYHPNVAAPGNSINF
LWMMAAHTEVADRQFGVVNVHPAYAAGGSGLEAAHETK

Sequences:

>Translated_278_residues
MSSLAPAINAQTCVIRATHKTKGRSRSLAPATAAVRHLHYGRIILDAADAPLEFSTGTHETGLIALNGHATIRLGAESFA
LGRYDAIYIPRDRAVVVTPGDSGCDLAEISAPVTGDYPVQYVPFSQVQSDPNLHFAAGAPSCERDLNIVFGKNVQAGRIM
AGVTFSKPGNWTSWPPHEHGRMLEEAYLYIDMPAPGWGLQLVFTNSNEPELVAKVGEGDVVVMPEGYHPNVAAPGNSINF
LWMMAAHTEVADRQFGVVNVHPAYAAGGSGLEAAHETK
>Mature_277_residues
SSLAPAINAQTCVIRATHKTKGRSRSLAPATAAVRHLHYGRIILDAADAPLEFSTGTHETGLIALNGHATIRLGAESFAL
GRYDAIYIPRDRAVVVTPGDSGCDLAEISAPVTGDYPVQYVPFSQVQSDPNLHFAAGAPSCERDLNIVFGKNVQAGRIMA
GVTFSKPGNWTSWPPHEHGRMLEEAYLYIDMPAPGWGLQLVFTNSNEPELVAKVGEGDVVVMPEGYHPNVAAPGNSINFL
WMMAAHTEVADRQFGVVNVHPAYAAGGSGLEAAHETK

Specific function: Involved in the isomerization of 5-deoxy-glucuronate (5DG) to 5-dehydro-2-deoxy-D-gluconate (DKG or 2-deoxy-5-keto-D- gluconate) [H]

COG id: COG3718

COG function: function code G; Uncharacterized enzyme involved in inositol metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isomerase iolB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR021120
- InterPro:   IPR014710 [H]

Pfam domain/function: PF04962 KduI [H]

EC number: 5.3.1.17 [C]

Molecular weight: Translated: 29680; Mature: 29549

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLAPAINAQTCVIRATHKTKGRSRSLAPATAAVRHLHYGRIILDAADAPLEFSTGTHE
CCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCEEECCCCCC
TGLIALNGHATIRLGAESFALGRYDAIYIPRDRAVVVTPGDSGCDLAEISAPVTGDYPVQ
CEEEEECCCEEEEECCCCEECCCEEEEEECCCCEEEEECCCCCCCHHHCCCCCCCCCCEE
YVPFSQVQSDPNLHFAAGAPSCERDLNIVFGKNVQAGRIMAGVTFSKPGNWTSWPPHEHG
EEEHHHHCCCCCEEEEECCCCCCCCCEEEECCCCCCCEEEEEEEECCCCCCCCCCCHHHC
RMLEEAYLYIDMPAPGWGLQLVFTNSNEPELVAKVGEGDVVVMPEGYHPNVAAPGNSINF
CEEEEEEEEEECCCCCCCEEEEEECCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCEEE
LWMMAAHTEVADRQFGVVNVHPAYAAGGSGLEAAHETK
EEEEEECCHHHHCCCCEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSLAPAINAQTCVIRATHKTKGRSRSLAPATAAVRHLHYGRIILDAADAPLEFSTGTHE
CCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCEEECCCCCC
TGLIALNGHATIRLGAESFALGRYDAIYIPRDRAVVVTPGDSGCDLAEISAPVTGDYPVQ
CEEEEECCCEEEEECCCCEECCCEEEEEECCCCEEEEECCCCCCCHHHCCCCCCCCCCEE
YVPFSQVQSDPNLHFAAGAPSCERDLNIVFGKNVQAGRIMAGVTFSKPGNWTSWPPHEHG
EEEHHHHCCCCCEEEEECCCCCCCCCEEEECCCCCCCEEEEEEEECCCCCCCCCCCHHHC
RMLEEAYLYIDMPAPGWGLQLVFTNSNEPELVAKVGEGDVVVMPEGYHPNVAAPGNSINF
CEEEEEEEEEECCCCCCCEEEEEECCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCEEE
LWMMAAHTEVADRQFGVVNVHPAYAAGGSGLEAAHETK
EEEEEECCHHHHCCCCEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-Deoxy-L-Threo-5-Hexosulose Uronate [C]

Specific reaction: 4-Deoxy-L-Threo-5-Hexosulose Uronate = 3-Deoxy-D-Glycero-2,5-Hexodiulosonate. [C]

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA