| Definition | Streptococcus pneumoniae D39, complete genome. |
|---|---|
| Accession | NC_008533 |
| Length | 2,046,115 |
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The map label for this gene is pflB [H]
Identifier: 116517188
GI number: 116517188
Start: 419643
End: 421967
Strand: Direct
Name: pflB [H]
Synonym: SPD_0420
Alternate gene names: 116517188
Gene position: 419643-421967 (Clockwise)
Preceding gene: 116516463
Following gene: 116515962
Centisome position: 20.51
GC content: 41.68
Gene sequence:
>2325_bases ATGGTTGTTAAGACAGTTGTTGAAGCACAAGATATTTTTGACAAAGCTTGGGAAGGCTTCAAAGGCGTAGATTGGAAAGA AAAAGCAAGTGTATCACGCTTTGTACAAGCTAACTACACACCTTATGATGGAGACGAAAGCTTCCTTGCAGGACCAACAG AGCGTTCACTTCACATCAAGAAAATTGTAGAAGAAACTAAAGCACACTACGAAGAAACTCGTTTCCCAATGGACACTCGT CCAACATCTATCGCTGATATCCCTGCTGGATTTATCGACAAAGAAAATGAAGTTATCTTTGGTATCCAAAATGATGAACT CTTCAAATTGAACTTCATGCCAAAAGGTGGTATCCGTATGGCTGAAACTACTTTAAAAGAAAATGGATACGAACCAGACC CAGCTGTTCACGAAATCTTCACTAAATATGTAACAACAGTTAACGACGGTATTTTCCGTGCCTACACTTCAAATATTCGT CGCGCTCGTCATGCACACACTGTAACTGGTCTTCCAGATGCATACTCACGCGGACGTATCATTGGTGTTTACGCACGTCT TGCTCTTTACGGTGCAGACTACTTGATGCAAGAAAAAGTAAATGACTGGAATGCAATCAAAGAAATCGATGAAGAAACAA TCCGTCTTCGTGAAGAAGTAAACCTTCAATACCAAGCATTGCAACAAGTTGTTCGCCTGGGTGACCTTTACGGGGTTGAT GTTCGCAAACCAGCGATGAACGTGAAAGAAGCAATCCAATGGGTTAACATTGCTTTCATGGCTGTCTGCCGTGTGATTAA CGGTGCTGCTACATCTCTAGGTCGTGTACCAATCGTATTGGACATCTTTGCAGAACGTGACCTTGCTCGTGGTACATTTA CTGAATCAGAAATCCAAGAATTCGTTGATGATTTCGTTATGAAACTTCGTACAGTTAAATTTGCTCGTACAAAAGCTTAT GACCAATTGTACTCAGGTGACCCAACCTTTATTACAACTTCTATGGCTGGTATGGGTAACGACGGTCGTCACCGTGTTAC TAAGATGGACTACCGTTTCTTGAACACTCTTGACAACATCGGTAACTCACCAGAACCAAACTTGACAGTTCTTTGGACTG ACAAATTGCCATACAACTTCCGTCGCTACTGTATGCACATGAGCCACAAACACTCTTCTATCCAATACGAAGGTGTAACA ACAATGGCTAAAGACGGATATGGTGAAATGAGCTGTATCTCATGCTGTGTGTCTCCACTTGATCCAGAAAATGAAGAACA ACGCCACAACATCCAGTACTTCGGTGCTCGTGTAAACGTTCTTAAAGCCCTTCTTACTGGTTTGAATGGTGGTTACGACG ATGTTCACAAAGACTACAAAGTATTTGATATCGAACCAATCCGTGACGAAGTTCTTGAATTCGAATCAGTTAAAGCGAAC TTTGAAAAATCTCTTGACTGGTTGACTGACACTTACGTAGATGCCTTGAACATCATCCACTACATGACTGATAGGTACAA CTACGAAGCTGTTCAAATGGCCTTCTTGCCAACTAAACAACGTGCCAACATGGGATTCGGTATCTGTGGATTTGCTAACA CTGTTGATACATTGTCAGCTATCAAATACGCTACAGTTAAACCAATCCGTGACGAAGATGGCTACATCTACGATTACGAA ACAATCGGTGACTACCCACGCTGGGGTGAAGATGACCCACGTTCAAACGAATTGGCAGAATGGTTGATCGAAGCTTACAC AACTCGTCTACGTAGCCACAAACTATACAAAGACGCAGAAGCTACAGTATCACTTTTGACAATCACATCTAACGTTGCTT ACTCTAAACAAACTGGTAACTCACCAGTTCACAAAGGTGTATACCTCAACGAAGATGGTTCTGTGAACTTGTCTAAACTT GAATTCTTCTCACCAGGTGCTAACCCATCTAACAAAGCTAAAGGTGGTTGGTTGCAAAACTTGAACTCACTTTCTAGCCT TGACTTTAGTTATGCAGCTGACGGTATCTCATTGACTACACAAGTATCACCTCGCGCTCTTGGTAAGACTCGTGATGAAC AAGTTGATAACTTGGTAACAATCCTTGATGGTTACTTCGAAAACGGTGGACAACACGTTAACTTGAACGTTATGGACTTG AACGATGTTTACGAAAAAATCATGTCAGGTGAAGACGTTATCGTACGTATCTCTGGATACTGTGTAAATACTAAGTACCT CACTCCAGAACAAAAAACTGAATTGACACAACGTGTCTTCCACGAAGTTCTTTCAATGGATGATGCCTTGGATGCATTGA GCTAA
Upstream 100 bases:
>100_bases AAAAATAGGGATTTTGCACTTGTTGAAATCGGTTACTTTATGGTATACTTGTCTTATGAATGTAACAGATGACTGTTACT AGAAAAAAGAGGACATTAAT
Downstream 100 bases:
>100_bases TCAAGTTTTTGAATAATGAAAAGGAACCCTCGGTCAATCGACTGAGGGTTTTGTGCTTGGGATAGTGTGAGCAATTCCTT CGGCGCAATATGCAATGTTT
Product: formate acetyltransferase
Products: NA
Alternate protein names: Pyruvate formate-lyase [H]
Number of amino acids: Translated: 774; Mature: 774
Protein sequence:
>774_residues MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDGDESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTR PTSIADIPAGFIDKENEVIFGIQNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLREEVNLQYQALQQVVRLGDLYGVD VRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRVPIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAY DQLYSGDPTFITTSMAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHSSIQYEGVT TMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNGGYDDVHKDYKVFDIEPIRDEVLEFESVKAN FEKSLDWLTDTYVDALNIIHYMTDRYNYEAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYE TIGDYPRWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVHKGVYLNEDGSVNLSKL EFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQVSPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDL NDVYEKIMSGEDVIVRISGYCVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS
Sequences:
>Translated_774_residues MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDGDESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTR PTSIADIPAGFIDKENEVIFGIQNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLREEVNLQYQALQQVVRLGDLYGVD VRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRVPIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAY DQLYSGDPTFITTSMAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHSSIQYEGVT TMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNGGYDDVHKDYKVFDIEPIRDEVLEFESVKAN FEKSLDWLTDTYVDALNIIHYMTDRYNYEAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYE TIGDYPRWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVHKGVYLNEDGSVNLSKL EFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQVSPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDL NDVYEKIMSGEDVIVRISGYCVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS >Mature_774_residues MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDGDESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTR PTSIADIPAGFIDKENEVIFGIQNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLREEVNLQYQALQQVVRLGDLYGVD VRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRVPIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAY DQLYSGDPTFITTSMAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHSSIQYEGVT TMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNGGYDDVHKDYKVFDIEPIRDEVLEFESVKAN FEKSLDWLTDTYVDALNIIHYMTDRYNYEAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYE TIGDYPRWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVHKGVYLNEDGSVNLSKL EFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQVSPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDL NDVYEKIMSGEDVIVRISGYCVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS
Specific function: Glucose metabolism (nonoxidative conversion). [C]
COG id: COG1882
COG function: function code C; Pyruvate-formate lyase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 pyruvate formate lyase domain [H]
Homologues:
Organism=Escherichia coli, GI1787131, Length=767, Percent_Identity=43.8070404172099, Blast_Score=620, Evalue=1e-178, Organism=Escherichia coli, GI48994926, Length=771, Percent_Identity=41.1154345006485, Blast_Score=576, Evalue=1e-165,
Paralogues:
None
Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR005949 - InterPro: IPR001150 - InterPro: IPR019777 - InterPro: IPR004184 [H]
Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]
EC number: =2.3.1.54 [H]
Molecular weight: Translated: 87823; Mature: 87823
Theoretical pI: Translated: 4.88; Mature: 4.88
Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.9 %Cys (Translated Protein) 2.7 %Met (Translated Protein) 3.6 %Cys+Met (Translated Protein) 0.9 %Cys (Mature Protein) 2.7 %Met (Mature Protein) 3.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDGDESFLAGPTERSLHIK CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHH KIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGIQNDELFKLNFMPKGGIRM HHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCH AETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIRRARHAHTVTGLPDAYSRGRI HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCE IGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLREEVNLQYQALQQVVRLGDLYGVD EHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC VRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRVPIVLDIFAERDLARGTFTESEIQE CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHCCCCCCCCCCHHHHHH FVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTSMAGMGNDGRHRVTKMDYRFLNTLDNI HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHC GNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHSSIQYEGVTTMAKDGYGEMSCISCCVSPL CCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEECCCEEHHCCCCCCHHHHHHHCCCC DPENEEQRHNIQYFGARVNVLKALLTGLNGGYDDVHKDYKVFDIEPIRDEVLEFESVKAN CCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEECCHHHHHHHHHHHHHHH FEKSLDWLTDTYVDALNIIHYMTDRYNYEAVQMAFLPTKQRANMGFGICGFANTVDTLSA HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHCCCCEEECCCHHHHHHHHH IKYATVKPIRDEDGYIYDYETIGDYPRWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAE HHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHH ATVSLLTITSNVAYSKQTGNSPVHKGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQN HEEEEEEEECCCEECCCCCCCCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHH LNSLSSLDFSYAADGISLTTQVSPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDL HHHHHHCCHHHCCCCEEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEH NDVYEKIMSGEDVIVRISGYCVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS HHHHHHHHCCCCEEEEEEEEEEECEECCCHHHHHHHHHHHHHHHHHHHHHHHCC >Mature Secondary Structure MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDGDESFLAGPTERSLHIK CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHH KIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGIQNDELFKLNFMPKGGIRM HHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCH AETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIRRARHAHTVTGLPDAYSRGRI HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCE IGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLREEVNLQYQALQQVVRLGDLYGVD EHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC VRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRVPIVLDIFAERDLARGTFTESEIQE CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHCCCCCCCCCCHHHHHH FVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTSMAGMGNDGRHRVTKMDYRFLNTLDNI HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHC GNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHSSIQYEGVTTMAKDGYGEMSCISCCVSPL CCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEECCCEEHHCCCCCCHHHHHHHCCCC DPENEEQRHNIQYFGARVNVLKALLTGLNGGYDDVHKDYKVFDIEPIRDEVLEFESVKAN CCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEECCHHHHHHHHHHHHHHH FEKSLDWLTDTYVDALNIIHYMTDRYNYEAVQMAFLPTKQRANMGFGICGFANTVDTLSA HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHCCCCEEECCCHHHHHHHHH IKYATVKPIRDEDGYIYDYETIGDYPRWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAE HHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHH ATVSLLTITSNVAYSKQTGNSPVHKGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQN HEEEEEEEECCCEECCCCCCCCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHH LNSLSSLDFSYAADGISLTTQVSPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDL HHHHHHCCHHHCCCCEEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEH NDVYEKIMSGEDVIVRISGYCVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS HHHHHHHHCCCCEEEEEEEEEEECEECCCHHHHHHHHHHHHHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8550181; 12397186 [H]