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The map label for this gene is thrB

Identifier: 116517101

GI number: 116517101

Start: 1222376

End: 1223245

Strand: Reverse

Name: thrB

Synonym: SPD_1194

Alternate gene names: 116517101

Gene position: 1223245-1222376 (Counterclockwise)

Preceding gene: 116516244

Following gene: 116516363

Centisome position: 59.78

GC content: 45.86

Gene sequence:

>870_bases
ATGAAGATTATTGTACCTGCAACCAGTGCCAATATCGGGCCAGGTTTTGACTCGGTCGGTGTAGCTGTAACCAAGTATCT
TCAAATTGAGGTCTGCGAAGAACGAGATGAGTGGCTGATTGAACACCAGATTGGCAAATGGATTCCACATGACGAGCGTA
ATCTCTTGCTCAAAATCGCTTTGCAAATTGTACCAGACTTGCAACCAAGACGCTTGAAAATGACCAGTGATGTCCCTTTG
GCGCGCGGTTTGGGTTCTTCCAGCTCGGTTATCGTTGCTGGGATTGAACTAGCCAACCAACTGGGTCAACTCAACTTATC
AGACCATGAAAAATTGCAGTTAGCGACCAAGATTGAAGGGCATCCTGACAATGTGGCTCCAGCCATTTATGGTAATCTCG
TTATTGCAAGTTCTGTTGAAGGGCAAGTCTCTGCTATCGTAGCAGACTTTCCAGAGTGTGATTTTCTAGCTTACATTCCA
AACTATGAATTACGTACTCGCGACAGCCGTAGTGTCTTGCCTAAAAAATTGTCTTATAAGGAAGCTGTTGCTGCAAGTTC
TATCGCCAATGTAGCGGTTGCTGCCTTGTTGGCAGGAGACATGGTGACCGCTGGGCAAGCAATCGAGGGAGACCTCTTCC
ATGAGCGCTATCGTCAGGACTTGGTAAGAGAATTTGCGATGATTAAGCAAGTGACCAAAGAAAATGGGGCCTATGCAACC
TACCTTTCTGGTGCTGGGCCGACAGTTATGGTTCTGGCTTCTCATGACAAGATGCCAACAATTAAGGCAGAATTGGAAAA
GCAACCTTTCAAAGGAAAACTGCATGACTTGAGAGTTGATACCCAAGGTGTCCGTGTAGAAGCAAAATAA

Upstream 100 bases:

>100_bases
CACAAGATTAATAAAGCCCAGCTTGAAAATGTCTCAGCTGAATTGAAGAAGGTTTCAGAATTCGACCTCTTGAATACCTT
CAAGGTGCTAGGAGAATAAG

Downstream 100 bases:

>100_bases
AGAATAGAATATAGGATGGGGAAACTCTTGACCAGAGGGGTTCATATCCTTTTTGTGAAAAGAAGTTTATACTCAATGAA
AATCAAAGAGCAAACTAGGA

Product: homoserine kinase

Products: NA

Alternate protein names: HK; HSK

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQIGKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPL
ARGLGSSSSVIVAGIELANQLGQLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYRQDLVREFAMIKQVTKENGAYAT
YLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHDLRVDTQGVRVEAK

Sequences:

>Translated_289_residues
MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQIGKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPL
ARGLGSSSSVIVAGIELANQLGQLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYRQDLVREFAMIKQVTKENGAYAT
YLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHDLRVDTQGVRVEAK
>Mature_289_residues
MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQIGKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPL
ARGLGSSSSVIVAGIELANQLGQLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYRQDLVREFAMIKQVTKENGAYAT
YLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHDLRVDTQGVRVEAK

Specific function: Catalyzes the ATP-dependent phosphorylation of L- homoserine to L-homoserine phosphate

COG id: COG0083

COG function: function code E; Homoserine kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. Homoserine kinase subfamily

Homologues:

Organism=Escherichia coli, GI1786184, Length=311, Percent_Identity=28.2958199356913, Blast_Score=99, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6321814, Length=320, Percent_Identity=28.125, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KHSE_STRP2 (Q04JY7)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816661.1
- ProteinModelPortal:   Q04JY7
- SMR:   Q04JY7
- STRING:   Q04JY7
- EnsemblBacteria:   EBSTRT00000020339
- GeneID:   4441912
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1194
- eggNOG:   COG0083
- GeneTree:   EBGT00050000027810
- HOGENOM:   HBG646290
- OMA:   FDCLGAA
- ProtClustDB:   PRK01212
- GO:   GO:0005737
- HAMAP:   MF_00384
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR006203
- InterPro:   IPR000870
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PIRSF:   PIRSF000676
- PRINTS:   PR00958
- TIGRFAMs:   TIGR00191

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.39

Molecular weight: Translated: 31549; Mature: 31549

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00627 GHMP_KINASES_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQIGKWIPHDERNLLLKIA
CEEEEECCCCCCCCCCCHHHHHHHHHEEEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHH
LQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLGQLNLSDHEKLQLATKIEG
HHHCCCCCCCEEEECCCCCHHHCCCCCCCEEEEEHHHHHHHCCCCCCCCHHEEEEEECCC
HPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIPNYELRTRDSRSVLPKKLSYK
CCCCCCHHHHCCEEEEECCCCCEEEEEECCCCCCEEEECCCCEEECCCCHHHCCHHHHHH
EAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYRQDLVREFAMIKQVTKENGAYAT
HHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
YLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHDLRVDTQGVRVEAK
EECCCCCEEEEEECCCCCCCHHHHHCCCCCCCCCEEEEECCCCEEEEEC
>Mature Secondary Structure
MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQIGKWIPHDERNLLLKIA
CEEEEECCCCCCCCCCCHHHHHHHHHEEEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHH
LQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLGQLNLSDHEKLQLATKIEG
HHHCCCCCCCEEEECCCCCHHHCCCCCCCEEEEEHHHHHHHCCCCCCCCHHEEEEEECCC
HPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIPNYELRTRDSRSVLPKKLSYK
CCCCCCHHHHCCEEEEECCCCCEEEEEECCCCCCEEEECCCCEEECCCCHHHCCHHHHHH
EAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYRQDLVREFAMIKQVTKENGAYAT
HHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
YLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHDLRVDTQGVRVEAK
EECCCCCEEEEEECCCCCCCHHHHHCCCCCCCCCEEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA