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The map label for this gene is lysS [H]

Identifier: 116517090

GI number: 116517090

Start: 640381

End: 641871

Strand: Direct

Name: lysS [H]

Synonym: SPD_0620

Alternate gene names: 116517090

Gene position: 640381-641871 (Clockwise)

Preceding gene: 116515805

Following gene: 116517149

Centisome position: 31.3

GC content: 43.46

Gene sequence:

>1491_bases
ATGTCTACAGAACATATGGAAGAACTAAATGACCAGCAGATCGTTCGCCGTGAAAAAATGGCTGCGCTCCGCGAACAAGG
AATCGATCCTTTCGGAAAACGTTTTGAACGTACTGCAAATTCACAAGAATTAAAAGATAAATATGCCAACCTCGATAAAG
AACAATTACACGATAAAAACGAAACAGCTACTATCGCAGGACGCTTGGTAACCAAACGTGGTAAAGGAAAAGTTGGTTTT
GCCCACCTTCAAGACCGCGAAGGCCAGATTCAGATCTATGTTCGTAAGGATGCTGTCGGTGAAGAAAACTACGAAATCTT
CAAAAAAGCAGACCTTGGTGACTTCCTTGGTGTCGAAGGTGAAGTGATGCGTACGGATATGGGAGAACTCTCTATCAAGG
CAACCCACATCACACACTTGTCTAAGGCTCTTCGTCCTCTTCCTGAGAAATTCCATGGTTTGACAGACGTTGAAACAATT
TACCGTAAACGTTACCTTGACTTGATTTCTAATCGTGAAAGCTTTGAACGCTTTGTCACTCGTTCAAAAATCATCTCTGA
AATCCGTCGTTACCTTGACCAAAAAGGATTCCTTGAAGTGGAAACACCTGTTCTTCATAATGAAGCCGGTGGAGCTGCTG
CCCGTCCATTTATCACCCACCACAATGCCCAAAACATTGACATGGTGCTTCGTATCGCGACTGAGCTTCACTTAAAACGC
CTTATCGTGGGTGGTATGGAACGTGTCTATGAAATTGGCCGTATCTTCCGTAACGAAGGAATGGATGCTACTCATAACCC
TGAGTTCACTTCTATCGAAGTTTACCAAGCCTATGCAGACTTCCAAGATATCATGGACTTGACGGAAGGCATTATCCAAC
ATGCTGCTAAATCAGTCAAAGGTGATGGCCCAGTCAACTACCAAGGTACTGAAATCAAGATTAACGAACCATTTAAGCGT
GTTCATATGGTGGATGCTATCAGAGAAATTACTGGTGTCGATTTCTGGCAAGACATGACTTTGGAAGAAGCTAAAGCTAT
CGCTGCTGAGAAGAAAGTTCCAGTTGAGAAACACTACACTGAGGTTGGTCACATCATCAATGCCTTCTTTGAAGAGTTTG
TTGAAGAAACTTTAATCCAACCAACCTTTGTCTATGGACATCCAGTAGCTGTATCTCCACTCGCTAAGAAAAATCCTGAA
GACCAACGCTTTACTGACCGTTTCGAGCTCTTTATCATGACTAAGGAGTACGGTAATGCCTTTACTGAGCTCAACGACCC
AATCGACCAACTTAGCCGTTTTGAAGCCCAAGCTAAAGCCAAAGAACTTGGTGATGATGAAGCGACAGGAATCGACTATG
ACTACATTGAAGCCCTTGAATACGGTATGCCACCAACAGGTGGTTTGGGAATCGGTATCGACCGTCTCTGCATGCTCCTC
ACTGATACAACAACTATCCGTGATGTATTGCTCTTCCCAACAATGAAATAA

Upstream 100 bases:

>100_bases
TAAAGATTATCCGTGAATTTCTTGCTTATTTATGATAAAATGGGAGTATCGCAAAAAATGACTCATCGTATTCAATTTTG
AGTAAAACTAGGAGGATCCC

Downstream 100 bases:

>100_bases
ATTCTTATCCTCTGGCTCTTATCAGGGGATTTTTTGATTCAAAAAGAGACTGAATTTAAGGAGAAAATGAAGTGTAGTAT
ATTGAAGTTGAAATAGTACA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQELKDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGF
AHLQDREGQIQIYVRKDAVGEENYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFITHHNAQNIDMVLRIATELHLKR
LIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQAYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKR
VHMVDAIREITGVDFWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVSPLAKKNPE
DQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATGIDYDYIEALEYGMPPTGGLGIGIDRLCMLL
TDTTTIRDVLLFPTMK

Sequences:

>Translated_496_residues
MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQELKDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGF
AHLQDREGQIQIYVRKDAVGEENYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFITHHNAQNIDMVLRIATELHLKR
LIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQAYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKR
VHMVDAIREITGVDFWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVSPLAKKNPE
DQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATGIDYDYIEALEYGMPPTGGLGIGIDRLCMLL
TDTTTIRDVLLFPTMK
>Mature_495_residues
STEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQELKDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGFA
HLQDREGQIQIYVRKDAVGEENYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETIY
RKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFITHHNAQNIDMVLRIATELHLKRL
IVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQAYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRV
HMVDAIREITGVDFWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVSPLAKKNPED
QRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATGIDYDYIEALEYGMPPTGGLGIGIDRLCMLLT
DTTTIRDVLLFPTMK

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=498, Percent_Identity=42.9718875502008, Blast_Score=384, Evalue=1e-107,
Organism=Homo sapiens, GI5031815, Length=498, Percent_Identity=42.9718875502008, Blast_Score=384, Evalue=1e-106,
Organism=Homo sapiens, GI40789249, Length=252, Percent_Identity=25.7936507936508, Blast_Score=82, Evalue=8e-16,
Organism=Escherichia coli, GI1789256, Length=504, Percent_Identity=50, Blast_Score=476, Evalue=1e-135,
Organism=Escherichia coli, GI1790571, Length=491, Percent_Identity=49.6945010183299, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI87082379, Length=327, Percent_Identity=30.5810397553517, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1788173, Length=255, Percent_Identity=29.0196078431373, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17535925, Length=499, Percent_Identity=44.8897795591182, Blast_Score=403, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17535927, Length=499, Percent_Identity=44.8897795591182, Blast_Score=403, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI71994340, Length=499, Percent_Identity=44.8897795591182, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI32566633, Length=295, Percent_Identity=26.7796610169492, Blast_Score=88, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6320242, Length=491, Percent_Identity=45.8248472505092, Blast_Score=401, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6324256, Length=480, Percent_Identity=34.5833333333333, Blast_Score=237, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6321807, Length=453, Percent_Identity=23.3995584988962, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325153, Length=136, Percent_Identity=30.1470588235294, Blast_Score=63, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24640849, Length=489, Percent_Identity=43.558282208589, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24640851, Length=489, Percent_Identity=43.558282208589, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24584738, Length=307, Percent_Identity=25.4071661237785, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 56682; Mature: 56551

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQELKDKYANLDKEQLHDKN
CCCHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCHHHHCCCC
ETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEENYEIFKKADLGDFLGVEG
CHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHCCCHHHHCCCC
EVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETIYRKRYLDLISNRESFERFVT
CEECCCCCCCEEEHHHHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHH
RSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFITHHNAQNIDMVLRIATELHLKR
HHHHHHHHHHHHCCCCCEEECCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQAYADFQDIMDLTEGIIQHAAKSVK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDGPVNYQGTEIKINEPFKRVHMVDAIREITGVDFWQDMTLEEAKAIAAEKKVPVEKHYT
CCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHCCCCHHHHHH
EVGHIINAFFEEFVEETLIQPTFVYGHPVAVSPLAKKNPEDQRFTDRFELFIMTKEYGNA
HHHHHHHHHHHHHHHHHHCCCEEEECCCCEECCHHHCCCCCHHHHCEEEEEEEEHHHCCH
FTELNDPIDQLSRFEAQAKAKELGDDEATGIDYDYIEALEYGMPPTGGLGIGIDRLCMLL
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHH
TDTTTIRDVLLFPTMK
HCCHHHHHHHHCCCCC
>Mature Secondary Structure 
STEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQELKDKYANLDKEQLHDKN
CCHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCHHHHCCCC
ETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEENYEIFKKADLGDFLGVEG
CHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHCCCHHHHCCCC
EVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETIYRKRYLDLISNRESFERFVT
CEECCCCCCCEEEHHHHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHH
RSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFITHHNAQNIDMVLRIATELHLKR
HHHHHHHHHHHHCCCCCEEECCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQAYADFQDIMDLTEGIIQHAAKSVK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDGPVNYQGTEIKINEPFKRVHMVDAIREITGVDFWQDMTLEEAKAIAAEKKVPVEKHYT
CCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHCCCCHHHHHH
EVGHIINAFFEEFVEETLIQPTFVYGHPVAVSPLAKKNPEDQRFTDRFELFIMTKEYGNA
HHHHHHHHHHHHHHHHHHCCCEEEECCCCEECCHHHCCCCCHHHHCEEEEEEEEHHHCCH
FTELNDPIDQLSRFEAQAKAKELGDDEATGIDYDYIEALEYGMPPTGGLGIGIDRLCMLL
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHH
TDTTTIRDVLLFPTMK
HCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA