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The map label for this gene is prs2

Identifier: 116517035

GI number: 116517035

Start: 992815

End: 993774

Strand: Reverse

Name: prs2

Synonym: SPD_0980

Alternate gene names: 116517035

Gene position: 993774-992815 (Counterclockwise)

Preceding gene: 116516210

Following gene: 116515455

Centisome position: 48.57

GC content: 40.73

Gene sequence:

>960_bases
ATGTCAGATAGAAAAAACATGAAACTTTTCGCACTCAACTCTAACCAAGAGATTGCACAGAAAATTGCCCAAGCTGTTGG
TGTCCCACTTGGAAAACTATCATCACGTCAATTTTCAGACGGAGAAATCCAAGTAAATATCGAAGAAAGTGTCCGTGGTT
ATGATGTTTACATCATCCAATCAACAAGTTTCCCTGTCAACAACCACCTAATGGAATTGTTAATCATGGTCGATGCTTGT
GTGCGTGCAAGTGCCCACAGTATCAACGTTGTCCTTCCATATTTTGGCTATGCACGTCAAGACCGCATTGCTTGTCCTCG
TGAGCCACTTACAGCAAAACTAGTTGCCAATATGCTGGTTAAGGCTGGAGTTGATCGTATCCTGACTCTTGATTTGCATG
CCGTTCAGGTTCAAGGTTTCTTTGATATTCCAGTGGATAATCTTTTCACTGTTCCCCTATTCGCAAAACATTACTGCGAT
AAAGGATTGCTTGGTTCAGATGTTGTTGTCGTTAGCCCTAAAAATTCAGGTGTCAAACGTGCGCGTAGCCTGGCTGAATA
TCTTGATGCTCCTATCGCCATTATCGACTACCCTCAAGACGATGCAACTCGTAACGAAGGTTATATTATTGGTGATGTTG
AAGGTAAGAAAGCTATCTTGATTGATGATATTTTAAATACAGGACGTACCTTCTCTGAAGCTTCTAAAATCGTTGAACGT
GAAGGAGCTACAGAAATTTATGCTGTTTCTAGCCACGGTCTCTTCGTCGAGGGAGCTGCTGAGCTTCTTGACAATACTAA
TATTAAAGAAATTCTTGTGACTGATTCAGTAGCAACAAAAGAAAAAACTCCTAAAAATGTATGCTACATCACTGCTAGTG
AGTTAATTGGCGATGCTATCGTCCGTATTCACGAAAGAAAACCAGTCAGCCCACTCTTTGCCTACAATAAAAAGAAATAA

Upstream 100 bases:

>100_bases
TTTTTGGTAAAATAGAAAAGATAAAAATACACAAACCCTAGTTCATATATGTAAGGTAAATATAACTAGGATTTATAAAG
TTTACAGAGGACGGTCTATA

Downstream 100 bases:

>100_bases
GGTGATTCTTTGATTTATTTGGACAATGCTGCAACGACTCCTATGTCAGCAGTTGCTATTTCAGCTATGACCAAGGTTAT
GCAAGAAACCCACGGAAATC

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK 2; Phosphoribosyl pyrophosphate synthase 2; P-Rib-PP synthase 2; PRPP synthase 2

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQVNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDAC
VRASAHSINVVLPYFGYARQDRIACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKAILIDDILNTGRTFSEASKIVER
EGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDSVATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK

Sequences:

>Translated_319_residues
MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQVNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDAC
VRASAHSINVVLPYFGYARQDRIACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKAILIDDILNTGRTFSEASKIVER
EGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDSVATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK
>Mature_318_residues
SDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQVNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACV
RASAHSINVVLPYFGYARQDRIACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCDK
GLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKAILIDDILNTGRTFSEASKIVERE
GATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDSVATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family

Homologues:

Organism=Homo sapiens, GI4506127, Length=313, Percent_Identity=41.2140575079872, Blast_Score=238, Evalue=4e-63,
Organism=Homo sapiens, GI28557709, Length=313, Percent_Identity=41.2140575079872, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI4506129, Length=313, Percent_Identity=40.2555910543131, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI84875539, Length=316, Percent_Identity=39.873417721519, Blast_Score=233, Evalue=1e-61,
Organism=Homo sapiens, GI4506133, Length=343, Percent_Identity=34.9854227405248, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI194018537, Length=347, Percent_Identity=34.0057636887608, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI310128524, Length=147, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI310115209, Length=147, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI310118259, Length=147, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI310119946, Length=147, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1787458, Length=315, Percent_Identity=44.7619047619048, Blast_Score=259, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17554702, Length=313, Percent_Identity=40.5750798722045, Blast_Score=224, Evalue=6e-59,
Organism=Caenorhabditis elegans, GI25149168, Length=313, Percent_Identity=40.5750798722045, Blast_Score=224, Evalue=7e-59,
Organism=Caenorhabditis elegans, GI71989924, Length=313, Percent_Identity=40.5750798722045, Blast_Score=222, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17554704, Length=310, Percent_Identity=40.3225806451613, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17570245, Length=339, Percent_Identity=31.2684365781711, Blast_Score=171, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6319403, Length=313, Percent_Identity=39.6166134185303, Blast_Score=216, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6320946, Length=313, Percent_Identity=40.8945686900958, Blast_Score=216, Evalue=5e-57,
Organism=Saccharomyces cerevisiae, GI6321776, Length=312, Percent_Identity=36.5384615384615, Blast_Score=199, Evalue=5e-52,
Organism=Saccharomyces cerevisiae, GI6322667, Length=191, Percent_Identity=41.3612565445026, Blast_Score=149, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6324511, Length=108, Percent_Identity=40.7407407407407, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355239, Length=313, Percent_Identity=41.5335463258786, Blast_Score=232, Evalue=2e-61,
Organism=Drosophila melanogaster, GI45551540, Length=336, Percent_Identity=38.9880952380952, Blast_Score=220, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24651458, Length=350, Percent_Identity=34.2857142857143, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24651456, Length=350, Percent_Identity=34.2857142857143, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI281362873, Length=350, Percent_Identity=34.2857142857143, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24651454, Length=350, Percent_Identity=34.2857142857143, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI45552010, Length=195, Percent_Identity=40, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24651462, Length=195, Percent_Identity=40, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24651464, Length=195, Percent_Identity=40, Blast_Score=131, Evalue=7e-31,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KPRS2_STRPN (P65241)

Other databases:

- EMBL:   AE005672
- PIR:   F95126
- RefSeq:   NP_345567.1
- ProteinModelPortal:   P65241
- SMR:   P65241
- EnsemblBacteria:   EBSTRT00000027090
- GeneID:   931609
- GenomeReviews:   AE005672_GR
- KEGG:   spn:SP_1095
- TIGR:   SP_1095
- GeneTree:   EBGT00050000027352
- HOGENOM:   HBG519284
- OMA:   ELNTIFL
- ProtClustDB:   PRK02458
- BioCyc:   SPNE170187-1:SP_1095-MONOMER
- BRENDA:   2.7.6.1
- GO:   GO:0005737
- HAMAP:   MF_00583_B
- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836
- TIGRFAMs:   TIGR01251

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.7.6.1

Molecular weight: Translated: 35126; Mature: 34995

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00114 PRPP_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQVNIEESVRGYDVYIIQ
CCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHCCCCEEEEEEE
STSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDRIACPREPLTAKLVANMLV
ECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHH
KAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCDKGLLGSDVVVVSPKNSGVKR
HHCCCCEEEEEEEEEEEEEEEECCCCCEEEHHHHHHHHCCCCCCCCCEEEECCCCCCHHH
ARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKAILIDDILNTGRTFSEASKIVER
HHHHHHHHCCCEEEEECCCCCCCCCCCEEEEECCCCEEEEEHHHHHCCCCHHHHHHHHHH
EGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDSVATKEKTPKNVCYITASELIGDAI
CCCCEEEEEECCCEEEECHHHHHCCCCCEEEEEECCHHCCCCCCCCEEEEEHHHHHHHHH
VRIHERKPVSPLFAYNKKK
HEECCCCCCCHHHCCCCCC
>Mature Secondary Structure 
SDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQVNIEESVRGYDVYIIQ
CCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHCCCCEEEEEEE
STSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDRIACPREPLTAKLVANMLV
ECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHH
KAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCDKGLLGSDVVVVSPKNSGVKR
HHCCCCEEEEEEEEEEEEEEEECCCCCEEEHHHHHHHHCCCCCCCCCEEEECCCCCCHHH
ARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKAILIDDILNTGRTFSEASKIVER
HHHHHHHHCCCEEEEECCCCCCCCCCCEEEEECCCCEEEEEHHHHHCCCCHHHHHHHHHH
EGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDSVATKEKTPKNVCYITASELIGDAI
CCCCEEEEEECCCEEEECHHHHHCCCCCEEEEEECCHHCCCCCCCCEEEEEHHHHHHHHH
VRIHERKPVSPLFAYNKKK
HEECCCCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916