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The map label for this gene is leuB

Identifier: 116516851

GI number: 116516851

Start: 1145186

End: 1146223

Strand: Reverse

Name: leuB

Synonym: SPD_1115

Alternate gene names: 116516851

Gene position: 1146223-1145186 (Counterclockwise)

Preceding gene: 116515984

Following gene: 116515583

Centisome position: 56.02

GC content: 43.16

Gene sequence:

>1038_bases
ATGGCAAAGAAAATAGTAGCTCTAGTAGGAGACGGAATTGGCCCAGAAATCATGGAGGCTGGTTTAGAAGTTCTGGAGGC
TCTAGCTGAAAAAACAGGTTTTGACTATGAGATTGACAGACGACCGTTCGGAGGTGCAGATATTGATGCAGCAGGACCTC
CCTTACCTGATGAAACCCTTAAGGCAAGTAGGGAAGCAGATGCTATCCTACTAGTAGCTATCGGTAGTCCTCAGTATGAT
GGAGTAGCGGTTCGCCCTGAACAAGGCCTGATGGCTCTCCGTAAGGAACTCAATCTTTACGCTAATATTCGTCCTGTAAA
AATCTTTGACAGTCTCAAGTATTTGTCACCACTCAAACCGGAACGAATTTCTGGTGTAGACTTTGTCGTGGTGCGTGAAT
TGACAGGCGAGATTTACTTTGGAGATCATATTCTTGAAGAGCGCAAAGCGCGTGATATCAACGACTATAGCTATGAGGAA
GTGGAGCGGATTATTCGCAAAGCCTTTGAAATTGCAAGAAATCGCAGAAAAATCGTTACTAGTATCGATAAGCAAAATGT
TCTAGCGACCTCAAAACTCTGGCGGAAAGTAGCTGAGGAAGTCGCACAGGATTTCCCAGATGTAACCTTGGAACATCAGC
TGGTAGACTCAGCTGCTATGCTTATGATTACCAATCCTGCTAAGTTTGATGTTATTGTAACGGAGAATCTTTTTGGAGAT
ATTTTATCTGATGAATCAAGCGTCTTATCTGGTACACTTGGGGTTATGCCATCAGCCAGTCATTCTGAAAATGGACCAAG
TCTCTATGAACCTATTCACGGTTCAGCACCTGATATTGCAGGTCAAGGAATTGCCAATCCTATTTCCATGATTTTATCAG
TTGTCATGATGTTGAGAGATAGTTTCGGACGTTATGAGGATACAGAGCGTATCAAACGTGCTGTTGAGACAAGTCTGGCG
GCAGGAATTTTAACGAGAGATATAGGAGGTCAGGCTTCAACAAAGGAAATGATGGAAGCTATTATTGCAAGGTTATGA

Upstream 100 bases:

>100_bases
CCTATATAAACGCTAATACCTTTGTTCAAAAAGAGAATGCAGGTGAGATGGGACGCAGTGTTTCTTACCACGATATGCCT
AGTGTGTAAAGGAGAAGGCT

Downstream 100 bases:

>100_bases
AGTTAGACGAAAAAATTACTCTAGTCCTTTTGATTTGGAATGTCATCATTTTCTTGATTTATGGCATTGACAAATTCAAG
GCAAGAAGAAGAGCTTGGCG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFGGADIDAAGPPLPDETLKASREADAILLVAIGSPQYD
GVAVRPEQGLMALRKELNLYANIRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
VERIIRKAFEIARNRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSAAMLMITNPAKFDVIVTENLFGD
ILSDESSVLSGTLGVMPSASHSENGPSLYEPIHGSAPDIAGQGIANPISMILSVVMMLRDSFGRYEDTERIKRAVETSLA
AGILTRDIGGQASTKEMMEAIIARL

Sequences:

>Translated_345_residues
MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFGGADIDAAGPPLPDETLKASREADAILLVAIGSPQYD
GVAVRPEQGLMALRKELNLYANIRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
VERIIRKAFEIARNRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSAAMLMITNPAKFDVIVTENLFGD
ILSDESSVLSGTLGVMPSASHSENGPSLYEPIHGSAPDIAGQGIANPISMILSVVMMLRDSFGRYEDTERIKRAVETSLA
AGILTRDIGGQASTKEMMEAIIARL
>Mature_344_residues
AKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFGGADIDAAGPPLPDETLKASREADAILLVAIGSPQYDG
VAVRPEQGLMALRKELNLYANIRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEEV
ERIIRKAFEIARNRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSAAMLMITNPAKFDVIVTENLFGDI
LSDESSVLSGTLGVMPSASHSENGPSLYEPIHGSAPDIAGQGIANPISMILSVVMMLRDSFGRYEDTERIKRAVETSLAA
GILTRDIGGQASTKEMMEAIIARL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=347, Percent_Identity=33.4293948126801, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI28178821, Length=349, Percent_Identity=28.080229226361, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI28178816, Length=349, Percent_Identity=28.6532951289398, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI4758582, Length=257, Percent_Identity=29.5719844357977, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI28178819, Length=186, Percent_Identity=30.1075268817204, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI28178838, Length=230, Percent_Identity=29.1304347826087, Blast_Score=92, Evalue=5e-19,
Organism=Escherichia coli, GI87081683, Length=352, Percent_Identity=44.8863636363636, Blast_Score=290, Evalue=9e-80,
Organism=Escherichia coli, GI1788101, Length=354, Percent_Identity=35.8757062146893, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1787381, Length=373, Percent_Identity=30.0268096514745, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71986051, Length=347, Percent_Identity=32.8530259365994, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17505779, Length=349, Percent_Identity=30.6590257879656, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17550882, Length=361, Percent_Identity=28.808864265928, Blast_Score=117, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI25144293, Length=359, Percent_Identity=27.0194986072423, Blast_Score=117, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6319830, Length=352, Percent_Identity=46.875, Blast_Score=296, Evalue=4e-81,
Organism=Saccharomyces cerevisiae, GI6322097, Length=358, Percent_Identity=37.4301675977654, Blast_Score=185, Evalue=9e-48,
Organism=Saccharomyces cerevisiae, GI6324709, Length=346, Percent_Identity=35.5491329479769, Blast_Score=162, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6324291, Length=263, Percent_Identity=36.1216730038023, Blast_Score=131, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24643270, Length=349, Percent_Identity=35.243553008596, Blast_Score=184, Evalue=7e-47,
Organism=Drosophila melanogaster, GI24643268, Length=349, Percent_Identity=35.243553008596, Blast_Score=184, Evalue=9e-47,
Organism=Drosophila melanogaster, GI24661184, Length=354, Percent_Identity=30.7909604519774, Blast_Score=138, Evalue=5e-33,
Organism=Drosophila melanogaster, GI161078633, Length=362, Percent_Identity=32.0441988950276, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24650122, Length=362, Percent_Identity=32.0441988950276, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI161078637, Length=362, Percent_Identity=32.0441988950276, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI161078635, Length=362, Percent_Identity=32.0441988950276, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI161078639, Length=359, Percent_Identity=32.3119777158774, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI281362242, Length=346, Percent_Identity=29.4797687861272, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24648872, Length=346, Percent_Identity=29.4797687861272, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI20130355, Length=344, Percent_Identity=24.4186046511628, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_STRR6 (Q8DPJ4)

Other databases:

- EMBL:   AE007317
- PIR:   F98013
- RefSeq:   NP_358728.1
- ProteinModelPortal:   Q8DPJ4
- SMR:   Q8DPJ4
- STRING:   Q8DPJ4
- EnsemblBacteria:   EBSTRT00000014868
- GeneID:   933207
- GenomeReviews:   AE007317_GR
- KEGG:   spr:spr1135
- eggNOG:   COG0473
- GeneTree:   EBGT00050000029341
- HOGENOM:   HBG518924
- OMA:   NTMIYSE
- PhylomeDB:   Q8DPJ4
- ProtClustDB:   PRK00772
- BioCyc:   SPNE171101:SPR1135-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 37859; Mature: 37728

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00470 IDH_IMDH; PS00230 MAP1B_NEURAXIN

Important sites: BINDING 94-94 BINDING 104-104 BINDING 132-132 BINDING 216-216

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFGGADIDAAGPPLPDETL
CCCCEEEHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCHHHH
KASREADAILLVAIGSPQYDGVAVRPEQGLMALRKELNLYANIRPVKIFDSLKYLSPLKP
HHCCCCCEEEEEEECCCCCCCEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
ERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEEVERIIRKAFEIARNRRKIVT
CCCCCCCEEEEEHHCCCEEECHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSAAMLMITNPAKFDVIVTENLFGD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEEEEECCCEEEEEEEHHHHHH
ILSDESSVLSGTLGVMPSASHSENGPSLYEPIHGSAPDIAGQGIANPISMILSVVMMLRD
HHCCCHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
SFGRYEDTERIKRAVETSLAAGILTRDIGGQASTKEMMEAIIARL
HCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
AKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFGGADIDAAGPPLPDETL
CCCEEEHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCHHHH
KASREADAILLVAIGSPQYDGVAVRPEQGLMALRKELNLYANIRPVKIFDSLKYLSPLKP
HHCCCCCEEEEEEECCCCCCCEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
ERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEEVERIIRKAFEIARNRRKIVT
CCCCCCCEEEEEHHCCCEEECHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSAAMLMITNPAKFDVIVTENLFGD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEEEEECCCEEEEEEEHHHHHH
ILSDESSVLSGTLGVMPSASHSENGPSLYEPIHGSAPDIAGQGIANPISMILSVVMMLRD
HHCCCHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
SFGRYEDTERIKRAVETSLAAGILTRDIGGQASTKEMMEAIIARL
HCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11544234