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The map label for this gene is ycjW [H]

Identifier: 116516729

GI number: 116516729

Start: 1619519

End: 1620520

Strand: Reverse

Name: ycjW [H]

Synonym: SPD_1605

Alternate gene names: 116516729

Gene position: 1620520-1619519 (Counterclockwise)

Preceding gene: 116515634

Following gene: 116515832

Centisome position: 79.2

GC content: 31.34

Gene sequence:

>1002_bases
ATGAAAAATATAAATGGGAAAAAGGTAACTATTTATGATATTGCACGTTTATCAGGTTTTTCTCCAAAAACAGTTTCACG
AGTTATTAATGGTGGAGTAAATGTTAAAGAAGAAACTTATCAAGCGATTCAAAAAGTTATAGAGGAATTGTCGTATATTC
CAAATGCGTATGCAAAAAATTTAACTAAAAAAGAAGCTATAAATATATTGATTTCTGTAAAAAAAATCGACTCTTTTCCT
TTAATTTGGTTTCATACGCTGTTAGATAAAGTGTTACGGACGTGTAAAGAATTTGGTGTTAATGCTATAGTTGAGTACTT
TGGAGAGGAGGATACAATTAGTAATTCAATTATATCAAGCACAGGTAGCCTAGTAGATGGTGTTATTGTTTTTTATGAAA
GTGTAGATGATATCAGAATTCAGTATTTAAAGAAGAACCATATGCCTTTTCTTGTTTTCGGTGAATCTCAAACGTCTGGA
GTAGTCTATGTATCTAATAATAATTTTCAAGCTACTTATGATATGATGAAAGCTGTAACTGAAGAAAAATTTAAAAATAT
GTGGTTGCTTATGGGAGGAGAATCCCATGTTAATAAGGATCGTGAAAGAGGTGTGCGTTCTTTTCTGAATGATAAAAATT
ACTTTATGGATTTAAAAGTCATTTATGGCTTATCTACAATTGATTCAGTTTATTCTTATGCAATGCAGCACTTGACTACC
CAAAATTATCCGGACATTATTTTTGTTTCAGGAGATGAAAAGGTTCAGGGCTTGATTCGTGCGTGCTATGAGAAAGGAAT
TCTTATTCCAGATGATATTTCTATCATTGGATTTGATAATATTCCTATTTCTCAGTATTATACTCCAGCTTTGTCTACAA
TTGCTCCCAATTATGTTAAATTGGCTAAAGAAATGATAGAAGGTGTTTTAGCAATTATTAAAGGGGAAAGTGTCACATCT
GTTGAAGTTTCTCCTAAATTTGTTAGGAGACAGAGTTTCTAG

Upstream 100 bases:

>100_bases
TTTGTGAATTTGTGGATCTTAAATAATTTACTAAATTCTCTTGTTAAAAAAAATTAAATATTGTATAGTAATATTAGAAT
GTTCGTAATGGAGGACTGAA

Downstream 100 bases:

>100_bases
TATTGTTTAATTCTTGAAAATCCTAGTGGAAAAGATGGAGTGTTCTAAAATAAGTTCTGTTTAGCCTCCATTCAGTTTCT
ATTGAGTTGGATCCAATTTC

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQKVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFP
LIWFHTLLDKVLRTCKEFGVNAIVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDLKVIYGLSTIDSVYSYAMQHLTT
QNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISIIGFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTS
VEVSPKFVRRQSF

Sequences:

>Translated_333_residues
MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQKVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFP
LIWFHTLLDKVLRTCKEFGVNAIVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDLKVIYGLSTIDSVYSYAMQHLTT
QNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISIIGFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTS
VEVSPKFVRRQSF
>Mature_333_residues
MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQKVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFP
LIWFHTLLDKVLRTCKEFGVNAIVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDLKVIYGLSTIDSVYSYAMQHLTT
QNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISIIGFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTS
VEVSPKFVRRQSF

Specific function: Unknown

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787580, Length=318, Percent_Identity=28.6163522012579, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787948, Length=334, Percent_Identity=26.9461077844311, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1786540, Length=336, Percent_Identity=25, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789068, Length=335, Percent_Identity=23.5820895522388, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1790715, Length=320, Percent_Identity=25.3125, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1790369, Length=321, Percent_Identity=24.9221183800623, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI48994940, Length=326, Percent_Identity=22.0858895705521, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37660; Mature: 37660

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQKVIEELSYIPNAYAKN
CCCCCCCEEEEEEEHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHC
LTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNAIVEYFGEEDTISNSIISS
CHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHC
TGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSGVVYVSNNNFQATYDMMKAVT
CCHHHHHHHHEECCCCHHHHHHHHCCCCCEEEECCCCCCCEEEEECCCCHHHHHHHHHHH
EEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDLKVIYGLSTIDSVYSYAMQHLTT
HHHHHCEEEEECCCHHCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCC
QNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISIIGFDNIPISQYYTPALSTIAPNYVK
CCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHCHHHHHHCCHHHH
LAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF
HHHHHHHHHHHHHCCCCEEEEECCHHHHHHCCC
>Mature Secondary Structure
MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQKVIEELSYIPNAYAKN
CCCCCCCEEEEEEEHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHC
LTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNAIVEYFGEEDTISNSIISS
CHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHC
TGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSGVVYVSNNNFQATYDMMKAVT
CCHHHHHHHHEECCCCHHHHHHHHCCCCCEEEECCCCCCCEEEEECCCCHHHHHHHHHHH
EEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDLKVIYGLSTIDSVYSYAMQHLTT
HHHHHCEEEEECCCHHCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCC
QNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISIIGFDNIPISQYYTPALSTIAPNYVK
CCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHCHHHHHHCCHHHH
LAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF
HHHHHHHHHHHHHCCCCEEEEECCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]