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The map label for this gene is rpoE [H]

Identifier: 116516666

GI number: 116516666

Start: 443576

End: 444163

Strand: Direct

Name: rpoE [H]

Synonym: SPD_0441

Alternate gene names: 116516666

Gene position: 443576-444163 (Clockwise)

Preceding gene: 116515870

Following gene: 116515581

Centisome position: 21.68

GC content: 42.35

Gene sequence:

>588_bases
TTGGAATTAGAAGTATTTGCTGGGCAAGAAAAAAGTGAACTATCTATGATTGAGGTAGCGCGTGCTATCTTGGAACTTCG
TGGTCGCGATCATGAGATGCATTTTAGCGATCTTGTAAACGAAATTCAAAACTACCTTGGAACATCAAACAGCGATATCC
GCGAAGCTTTGCCTTTGTTCTACACAGAGTTGAACTTTGACGGTAGCTTCATCTCACTTGGGGACAACAAATGGGGTCTT
CGTTCATGGTATGGTGTGGACGAAATCGACGAAGAAATCATCGCTCTTGAAGAAAATGACGACGATGAAGTAGCACCAAA
AGCTAAGAAAAAACGTGTCAATGCCTTTATGGATGGTGATTCAGATGCCATTGACTACAATGCAGATGATCCAGAAGACG
AAGATGCATACGAAGCAGATCCAGCTCTTTCATACGATGATGAAAATCCAGATGATGAAAAAAATGAAGTGGAAGCTTAT
GATGCAGAAATCAACGAAATCGCTCCAGATGACTTGGGAGAAGATGTGGATCTCAACGAAGACGACGACGAGTTTTCAGA
TGACGACGCTGAAACCAGCGAGGAATAA

Upstream 100 bases:

>100_bases
TGACTATTCCGCAAAATTATCGTAAAATAAAGAGTAAATGATAAAATGAGGTCAGAGTCTGTTCGCTCTGGCGATAGTAG
TATAAATGAGGAGAAACGCT

Downstream 100 bases:

>100_bases
GAGTTAGCTATTGACAATTATCCTATTTTTAGGTATCATATTGTTTGGGCACCTCTTTTAGAGGTCGGGGCTCCCTAGTT
CTTAGGGAGCTATTTTTGTT

Product: DNA-directed RNA polymerase subunit delta

Products: diphosphate; RNAn+1

Alternate protein names: RNAP delta factor [H]

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQNYLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGL
RSWYGVDEIDEEIIALEENDDDEVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE

Sequences:

>Translated_195_residues
MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQNYLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGL
RSWYGVDEIDEEIIALEENDDDEVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE
>Mature_195_residues
MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQNYLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGL
RSWYGVDEIDEEIIALEENDDDEVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE

Specific function: Participates in both the initiation and recycling phases of transcription. In the presence of the delta subunit, RNAP displays an increased specificity of transcription, a decreased affinity for nucleic acids, and an increased efficiency of RNA synthesis

COG id: COG3343

COG function: function code K; DNA-directed RNA polymerase, delta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rpoE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007759 [H]

Pfam domain/function: PF05066 RNA_pol_delta [H]

EC number: 2.7.7.6

Molecular weight: Translated: 22125; Mature: 22125

Theoretical pI: Translated: 3.49; Mature: 3.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQNYLGTSNSDIREALPLF
CCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
YTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDDEVAPKAKKKRVNAFMDGD
HHEECCCCCEEEECCCCCCCHHCCCCCHHCHHEEEECCCCCCCCCCHHHHHHHHHHCCCC
SDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAYDAEINEIAPDDLGEDVDLNE
CCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
DDDEFSDDDAETSEE
CCCCCCCCCCCCCCC
>Mature Secondary Structure
MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQNYLGTSNSDIREALPLF
CCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
YTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDDEVAPKAKKKRVNAFMDGD
HHEECCCCCEEEECCCCCCCHHCCCCCHHCHHEEEECCCCCCCCCCHHHHHHHHHHCCCC
SDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAYDAEINEIAPDDLGEDVDLNE
CCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
DDDEFSDDDAETSEE
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nucleoside triphosphate; RNAn

Specific reaction: nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]