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The map label for this gene is thiI

Identifier: 116516659

GI number: 116516659

Start: 789665

End: 790879

Strand: Direct

Name: thiI

Synonym: SPD_0777

Alternate gene names: 116516659

Gene position: 789665-790879 (Clockwise)

Preceding gene: 116515406

Following gene: 116515554

Centisome position: 38.59

GC content: 42.96

Gene sequence:

>1215_bases
ATGCAGTATTCAGAAATTATGATTCGCTACGGAGAGTTGTCAACCAAGGGTAAAAACCGTATGCGTTTCATCAATAAACT
TCGTAATAATATTTCGGACGTTTTGTCTATCTATAGCCAAGTTAAGGTAACAGCAGATCGCGACCGTGCCCACGCTTACC
TCAATGGAGCTGATTACACAGCAGTTGCAGAATCTCTCAAACAAGTTTTTGGAATTCAAAACTTTTCTCCTGTTTATAAG
GTTGAAAAATCTGTAGAAGTTTTGAAGTCTTCTGTCCAAGAGATTATGCGGGACATCTACAAGGAAGGTATGACCTTTAA
GATTTCTAGCAAGCGTAGCGACCACAACTTTGAACTTGATAGTCGTGAACTCAACCAAACACTTGGAGGGGCTGTATTCG
AAGCCATTCCAAATGTGCAAGTTCAAATGAAAAGTCCTGACATCAATCTTCAGGTGGAGATTCGTGAAGAAGCAGCCTAT
CTTTCTTATGAAACCATTCGTGGGGCTGGTGGTTTGCCAGTTGGAACTTCAGGTAAAGGGATGCTCATGTTGTCAGGAGG
GATTGACTCACCTGTAGCAGGTTATCTTGCTCTTAAGCGTGGGGTGGATATCGAGGCAGTTCACTTTGCTAGTCCACCAT
ATACTAGTCCTGGTGCCCTCAAGAAAGCGCAGGACTTGACTCGTAAATTGACCAAGTTTGGCGGAAATATCCAGTTTATC
GAGGTGCCTTTCACAGAGATTCAAGAGGAAATCAAAGCCAAAGCGCCAGAAGCTTATTTGATGACTCTAACTCGTCGCTT
TATGATGCGGATTACTGACCGTATTCGTGAGGTACGAAATGGTTTGGTTATCATCAATGGGGAAAGTCTAGGTCAAGTAG
CCAGCCAAACCCTTGAAAGTATGAAGGCTATCAATGCTGTTACCAACACTCCCATCATTCGTCCTGTGGTTACCATGGAC
AAGTTGGAAATCATTGACATCGCCCAGGAAATCGATACCTTTGACATTTCAATCCAACCGTTTGAAGACTGTTGTACCAT
TTTTGCACCAGATCGTCCAAAAACAAATCCTAAAATTAAGAATGCGGAGCAGTACGAAGCGCGTATGGATGTTGAAGGCT
TGGTTGAGCGAGCAGTGGCTGGAATCATGATTACTGAAATCACACCTCAAGCCGAAAAAGATGAAGTTGATGACTTGATT
GACAATCTGCTCTAA

Upstream 100 bases:

>100_bases
TTAGCCTAGACTTGGAAAATGATATGAGTCAGGTCGAGCAGTTTTTGACCAAGTTAAAATTGATTTACAATCAAACTAGA
AAAGTAAGATAGGAGCATTC

Downstream 100 bases:

>100_bases
TTCAGAAAATCCAAAAGAATAGCGAAAATCAGTAAAAAAAGTTAGTTTTTTCTCTAAAAACAGGTAAAAAACTAACTTTT
TTTATTTTTATGATATAATG

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYSQVKVTADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYK
VEKSVEVLKSSVQEIMRDIYKEGMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPGALKKAQDLTRKLTKFGGNIQFI
EVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIREVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMD
KLEIIDIAQEIDTFDISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAEKDEVDDLI
DNLL

Sequences:

>Translated_404_residues
MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYSQVKVTADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYK
VEKSVEVLKSSVQEIMRDIYKEGMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPGALKKAQDLTRKLTKFGGNIQFI
EVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIREVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMD
KLEIIDIAQEIDTFDISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAEKDEVDDLI
DNLL
>Mature_404_residues
MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYSQVKVTADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYK
VEKSVEVLKSSVQEIMRDIYKEGMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPGALKKAQDLTRKLTKFGGNIQFI
EVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIREVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMD
KLEIIDIAQEIDTFDISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAEKDEVDDLI
DNLL

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=374, Percent_Identity=27.0053475935829, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_STRP2 (Q04L36)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816262.1
- ProteinModelPortal:   Q04L36
- SMR:   Q04L36
- STRING:   Q04L36
- EnsemblBacteria:   EBSTRT00000018823
- GeneID:   4443063
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_0777
- eggNOG:   COG0301
- GeneTree:   EBGT00050000027439
- HOGENOM:   HBG646072
- OMA:   ESIGQVA
- ProtClustDB:   PRK01565
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP

EC number: =2.8.1.4

Molecular weight: Translated: 45147; Mature: 45147

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS51165 THUMP

Important sites: BINDING 265-265 BINDING 287-287 BINDING 296-296

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYSQVKVTADRDRAHAYLNGADYT
CCHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEECCCHHH
AVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKEGMTFKISSKRSDHNFELD
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCC
SRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAYLSYETIRGAGGLPVGTSGKG
HHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCHHHHHHHHHCCCCCCCCCCCCCE
MLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPGALKKAQDLTRKLTKFGGNIQFI
EEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEE
EVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIREVRNGLVIINGESLGQVASQTLES
ECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHH
MKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTFDISIQPFEDCCTIFAPDRPKTNPKIK
HHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCEEEEEECCHHHHHEEECCCCCCCCCCCC
NAEQYEARMDVEGLVERAVAGIMITEITPQAEKDEVDDLIDNLL
CCHHHHHHCCHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYSQVKVTADRDRAHAYLNGADYT
CCHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEECCCHHH
AVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKEGMTFKISSKRSDHNFELD
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCC
SRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAYLSYETIRGAGGLPVGTSGKG
HHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCHHHHHHHHHCCCCCCCCCCCCCE
MLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPGALKKAQDLTRKLTKFGGNIQFI
EEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEE
EVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIREVRNGLVIINGESLGQVASQTLES
ECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHH
MKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTFDISIQPFEDCCTIFAPDRPKTNPKIK
HHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCEEEEEECCHHHHHEEECCCCCCCCCCCC
NAEQYEARMDVEGLVERAVAGIMITEITPQAEKDEVDDLIDNLL
CCHHHHHHCCHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA