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The map label for this gene is iscS [C]

Identifier: 116515406

GI number: 116515406

Start: 788514

End: 789656

Strand: Direct

Name: iscS [C]

Synonym: SPD_0776

Alternate gene names: 116515406

Gene position: 788514-789656 (Clockwise)

Preceding gene: 116517016

Following gene: 116516659

Centisome position: 38.54

GC content: 44.09

Gene sequence:

>1143_bases
ATGATTTACTTTGATAATTCAGCGACGACCAAGCCTTATCCTGAAGCCCTTGAAACCTATATGCAGGTCGCTTCAAAAAT
TTTAGGAAACCCATCTAGTCTCCATCGTTTGGGAGACCAAGCAACACGAATCTTAGATGCTTCTCGCCAACAGATTGCAG
ATTTAATCGGTAAGAAAAGCGATGAAATCTTCTTTACCTCGGGTGGAACAGAAGGGGATAACTGGCTTATCAAGGGTGTG
GCCTTTGAAAAAGCTCAGTTTGGCAAGCACATCATTGTTTCAGCCATTGAACATCCAGCAGTCAAAGAGTCAGCCCTCTG
GTTGAAAAGTCAAGGATTTGAAGTGGATTTTGCTCCAGTTGATAAGAAAGGCTTGGTCGATGTTGAGGCGTTAGCAGGTT
TGATACGGCATGATACAATCCTCGTTTCCATCATGGCTGTGAACAATGAAATCGGCTCTATCCAACCTATTGAGGCTATT
TCAGAATTCTTGGCAGACAAGCCGACTATTTCCTTCCACGTTGATGCGGTTCAGGCGCTTGCCAAAATTCCGACTGAAAA
GTATCTGACAGAACGGGTGGATTGCGCGACTTTCTCTAGTCACAAGTTCCACGGGGTTCGAGGTGTTGGCTTTGTCTATA
TCAAATCTGGCAAGAAGATTACACCTCTTCTTACAGGTGGTGGCCAGGAGCGAGATTATCGTTCGACAACTGAAAATGTG
GCAGGGATTGCAGCGACAGCCAAGGCCCTCCGTTTGTCTATGGAAAAGCTAGATATCTTTAGGAGCAAGACTGGGCAGAT
GAAGGCAGTGATTCGCCAAGCTCTTCTGAACTATCCGGATATTTTTGTCTTTTCAGATGAGGAAAACTTTGCACCTCATA
TTCTGACTTTTGGAATCAAAGGTGTTCGAGGTGAAGTCATCGTTCACGCCTTTGAAGACTATGATATTTTCATCTCAACA
ACCTCAGCTTGTTCATCTAAGGCAGGAAAACCAGCCGGTACCTTGATTGCCATGGGAGTGGACAAAGATAAGGCCAAGTC
AGCTGTGCGTCTTAGCCTAGACTTGGAAAATGATATGAGTCAGGTCGAGCAGTTTTTGACCAAGTTAAAATTGATTTACA
ATCAAACTAGAAAAGTAAGATAG

Upstream 100 bases:

>100_bases
TTCTTTAAAAAAATCTTAAAAAAAGAGAAATTTCCCTTTCTTGTCGCAAGCTCCGTTTCATGATACAATAGAGGAAGTGA
TTTTAGAAAGCGTGAGAAAC

Downstream 100 bases:

>100_bases
GAGCATTCATGCAGTATTCAGAAATTATGATTCGCTACGGAGAGTTGTCAACCAAGGGTAAAAACCGTATGCGTTTCATC
AATAAACTTCGTAATAATAT

Product: aminotransferase, class V

Products: L-alanine; S [C]

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRILDASRQQIADLIGKKSDEIFFTSGGTEGDNWLIKGV
AFEKAQFGKHIIVSAIEHPAVKESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRHDTILVSIMAVNNEIGSIQPIEAI
SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGKKITPLLTGGGQERDYRSTTENV
AGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALLNYPDIFVFSDEENFAPHILTFGIKGVRGEVIVHAFEDYDIFIST
TSACSSKAGKPAGTLIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR

Sequences:

>Translated_380_residues
MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRILDASRQQIADLIGKKSDEIFFTSGGTEGDNWLIKGV
AFEKAQFGKHIIVSAIEHPAVKESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRHDTILVSIMAVNNEIGSIQPIEAI
SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGKKITPLLTGGGQERDYRSTTENV
AGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALLNYPDIFVFSDEENFAPHILTFGIKGVRGEVIVHAFEDYDIFIST
TSACSSKAGKPAGTLIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR
>Mature_380_residues
MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRILDASRQQIADLIGKKSDEIFFTSGGTEGDNWLIKGV
AFEKAQFGKHIIVSAIEHPAVKESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRHDTILVSIMAVNNEIGSIQPIEAI
SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGKKITPLLTGGGQERDYRSTTENV
AGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALLNYPDIFVFSDEENFAPHILTFGIKGVRGEVIVHAFEDYDIFIST
TSACSSKAGKPAGTLIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR

Specific function: Fe-S cluster biosynthesis [C]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=356, Percent_Identity=34.2696629213483, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI156713448, Length=409, Percent_Identity=31.7848410757946, Blast_Score=165, Evalue=6e-41,
Organism=Escherichia coli, GI48994898, Length=358, Percent_Identity=38.8268156424581, Blast_Score=231, Evalue=5e-62,
Organism=Escherichia coli, GI1787970, Length=364, Percent_Identity=23.9010989010989, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789175, Length=347, Percent_Identity=25.9365994236311, Blast_Score=78, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI25143064, Length=394, Percent_Identity=34.2639593908629, Blast_Score=194, Evalue=8e-50,
Organism=Caenorhabditis elegans, GI17533177, Length=318, Percent_Identity=27.9874213836478, Blast_Score=115, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6319831, Length=356, Percent_Identity=36.2359550561798, Blast_Score=206, Evalue=3e-54,
Organism=Drosophila melanogaster, GI20129463, Length=359, Percent_Identity=33.4261838440111, Blast_Score=191, Evalue=8e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: NA

Molecular weight: Translated: 41764; Mature: 41764

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRILDASRQQIADLIGKKS
CEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DEIFFTSGGTEGDNWLIKGVAFEKAQFGKHIIVSAIEHPAVKESALWLKSQGFEVDFAPV
CCEEEECCCCCCCCEEEEEEEHHHHHCCCHHHHHHHCCCCHHHHHHEEECCCCEEEECCC
DKKGLVDVEALAGLIRHDTILVSIMAVNNEIGSIQPIEAISEFLADKPTISFHVDAVQAL
CCCCCEEHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHH
AKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGKKITPLLTGGGQERDYRSTTENV
HHCCHHHHHHHHHCHHCCCCCCCCCCCCEEEEEEECCCEEEEEEECCCCCCCHHHHHHHH
AGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALLNYPDIFVFSDEENFAPHILTFGIK
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCC
GVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGTLIAMGVDKDKAKSAVRLSLDLENDMS
CCCCEEEEEEECCCEEEEEECHHHHCCCCCCCCEEEEECCCCHHCCCEEEEEEECHHHHH
QVEQFLTKLKLIYNQTRKVR
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRILDASRQQIADLIGKKS
CEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DEIFFTSGGTEGDNWLIKGVAFEKAQFGKHIIVSAIEHPAVKESALWLKSQGFEVDFAPV
CCEEEECCCCCCCCEEEEEEEHHHHHCCCHHHHHHHCCCCHHHHHHEEECCCCEEEECCC
DKKGLVDVEALAGLIRHDTILVSIMAVNNEIGSIQPIEAISEFLADKPTISFHVDAVQAL
CCCCCEEHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHH
AKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGKKITPLLTGGGQERDYRSTTENV
HHCCHHHHHHHHHCHHCCCCCCCCCCCCEEEEEEECCCEEEEEEECCCCCCCHHHHHHHH
AGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALLNYPDIFVFSDEENFAPHILTFGIK
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCC
GVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGTLIAMGVDKDKAKSAVRLSLDLENDMS
CCCCEEEEEEECCCEEEEEECHHHHCCCCCCCCEEEEECCCCHHCCCEEEEEEECHHHHH
QVEQFLTKLKLIYNQTRKVR
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-cysteine [C]

Specific reaction: L-cysteine = L-alanine + S [C]

General reaction: transfers sulfane sulfur to ThiI during in vitro biosynthesis of 4-thiouridine in E. coli tRNA [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8031904 [H]