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The map label for this gene is dpnC

Identifier: 116516583

GI number: 116516583

Start: 1645203

End: 1645967

Strand: Reverse

Name: dpnC

Synonym: SPD_1631

Alternate gene names: 116516583

Gene position: 1645967-1645203 (Counterclockwise)

Preceding gene: 116516006

Following gene: 116515864

Centisome position: 80.44

GC content: 32.55

Gene sequence:

>765_bases
ATGGAATTACACTTTAATTTAGAATTAGTAGAAACCTATAAAAGTAATAGCCAAAAGGCACGTATCTTAACGGAGGATTG
GGTTTATCGTCAGTCTTATTGCCCTAATTGTGGGAACAATCCCTTAAATCATTTTGAAAATAATCGGCCTGTAGCAGATT
TTTACTGTAATCATTGTAGTGAGGAGTTTGAACTAAAGAGCAAAAAAGGAAATTTTTCATCAACAATCAATGATGGTGCT
TATGCAACGATGATGAAGCGTGTGCAGGCAGATAATAATCCTAATTTCTTTTTTTTAACTTACACAAAAAATTTTGAGGT
AAATAACTTTCTTGTCCTTCCGAAGCAATTTGTTACACCGAAATCGATTATTCAAAGAAAACCACTTGCACCAACTGCTA
GACGAGCAGGTTGGATTGGTTGTAACATTGATTTATCACAAGTACCTTCTAAAGGAAGGATATTTCTTGTGCAAGATGGA
CAAGTTAGAGATCCAGAAAAAGTTACAAAAGAATTTAAGCAAGGTTTATTTTTAAGGAAGAGCTCTCTGTCATCAAGAGG
TTGGACAATAGAAATTCTAAATTGTATAGATAAGATAGAGGGTTCAGAATTTACCCTTGAAGATATGTATCGTTTTGAAA
GTGACCTAAAAAATATCTTTGTTAAGAACAATCATATCAAAGAAAAGATTAGGCAACAGCTTCAAATATTAAGAGACAAA
GAAATAATAGAATTTAAAGGTAGAGGAAAGTATCGGAAATTATGA

Upstream 100 bases:

>100_bases
GAAATACAAATGTAATAGGTACATATATGTGGTATACTAGTTATAGTAGATTTTAATTGCTTATATCGAGTGAGTTCTCA
TATTAGTGAGGAATTTTTAA

Downstream 100 bases:

>100_bases
AAACGAAACAACTTGTTGCATCAGAAGAGGTGTATGATTTCTTAAAAGTCATCTGGCCTGATTATGAAACTGAAAGCCGT
TACGATAACCTAAGTTTAAT

Product: Dam-replacing family protein

Products: NA

Alternate protein names: R.DpnI; Endonuclease DpnI; Type II restriction enzyme DpnI

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNHFENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGA
YATMMKRVQADNNPNFFFLTYTKNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKNIFVKNNHIKEKIRQQLQILRDK
EIIEFKGRGKYRKL

Sequences:

>Translated_254_residues
MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNHFENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGA
YATMMKRVQADNNPNFFFLTYTKNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKNIFVKNNHIKEKIRQQLQILRDK
EIIEFKGRGKYRKL
>Mature_254_residues
MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNHFENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGA
YATMMKRVQADNNPNFFFLTYTKNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKNIFVKNNHIKEKIRQQLQILRDK
EIIEFKGRGKYRKL

Specific function: Recognizes the double-stranded and methylated sequence G(Me)ATC and cleaves after A-2

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): T2D1_STRPN (P0A459)

Other databases:

- EMBL:   M14340
- EMBL:   AE005672
- PIR:   A31975
- PIR:   A95216
- RefSeq:   NP_346282.1
- REBASE:   5187
- REBASE:   776
- EnsemblBacteria:   EBSTRT00000026492
- GeneID:   930966
- GenomeReviews:   AE005672_GR
- KEGG:   spn:SP_1850
- TIGR:   SP_1850
- GeneTree:   EBGT00050000030012
- HOGENOM:   HBG302213
- OMA:   QYELKSK
- ProtClustDB:   CLSK878276
- BioCyc:   SPNE170187-1:SP_1850-MONOMER
- BRENDA:   3.1.21.4
- InterPro:   IPR010324

Pfam domain/function: PF06044 DRP

EC number: =3.1.21.4

Molecular weight: Translated: 29785; Mature: 29785

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNHFENNRPVADFYCNHCS
CEEEEEHHEEEHHHCCCCCEEEEHHHHHHHHHCCCCCCCCCHHHHCCCCCHHHHHHHCCC
EEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYTKNFEVNNFLVLPKQFVTP
HHHEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEECCHHHCCH
KSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDGQVRDPEKVTKEFKQGLFLRK
HHHHHCCCCCCHHHHCCEEECCCCHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCEEEC
SSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKNIFVKNNHIKEKIRQQLQILRDK
CCCCCCCCEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
EIIEFKGRGKYRKL
CEEEECCCCCCCCC
>Mature Secondary Structure
MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNHFENNRPVADFYCNHCS
CEEEEEHHEEEHHHCCCCCEEEEHHHHHHHHHCCCCCCCCCHHHHCCCCCHHHHHHHCCC
EEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYTKNFEVNNFLVLPKQFVTP
HHHEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEECCHHHCCH
KSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDGQVRDPEKVTKEFKQGLFLRK
HHHHHCCCCCCHHHHCCEEECCCCHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCEEEC
SSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKNIFVKNNHIKEKIRQQLQILRDK
CCCCCCCCEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
EIIEFKGRGKYRKL
CEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2844782; 3019562; 11463916