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The map label for this gene is wcaL [C]

Identifier: 116516575

GI number: 116516575

Start: 1229063

End: 1230163

Strand: Reverse

Name: wcaL [C]

Synonym: SPD_1200

Alternate gene names: 116516575

Gene position: 1230163-1229063 (Counterclockwise)

Preceding gene: 116515753

Following gene: 116516760

Centisome position: 60.12

GC content: 37.06

Gene sequence:

>1101_bases
GTGGCTAAGAAAAAAATCTTATTTTTTATGTGGTCTTTTTCTCTTGGAGGTGGTGCAGAGAAGATTCTATCAACCATTGT
TTCAAATCTGGATCCAGAAAAGTATGATATTGATATTCTTGAAATGGAGCACTTTGACAAGGGATATGAATCTGTTCCAA
AGCATGTACGCATTTTAAAATCCCTTCAAGATTATCGCCAAACCAGATGGTTACGAGCTTTTTTGTGGAGAATGAGAATT
TATTTTCCAAGACTGGCTCGTCGTTTGCTTGTAAAGGATGATTATGATGTTGAAGTTTCTTTTACCATTATGAATCCACC
ACTGTTGTTCTCTAAAAGAAGAGAAGTCAAGAAGATATCTTGGATTCATGGAAGTATTGAAGAACTTCTTAAGGATAGCT
CTAAAAGAGAATCACATAGAAGCCAGTTGGATGCTGCGAATACAATTGTAGGGATTTCAAAAAAGACCAGCAATTCTATC
AAGGAAGTTTATCCAGATTATACTTCTAAATTACAGACAATCTACAATGGATATGATTTTCAGACTATTCTAGAAAAATC
TCAAGAGAAGATCGATATCGAGATTGCTCCTCAAAGTATCTGTACTATCGGACGGATTGAGGAAAATAAGGGTTCTGACC
GTGTAGTGGAAGTGATACGATTATTACACCAAGAGGGAAAAAACTATCATCTCTATTTTATCGGGGCTGGTGATATGGAA
GAGGAACTGAAAAAACGAGTCAAAGAGTATGAGATTGAGGACTATGTACATTTCCTTGGTTATCAAAAAAATCCTTATCA
GTATTTATCTCAGACGAAAGTTCTCTTGTCTATGTCTAAACAAGAAGGTTTTCCTGGAGTGTATGTGGAGGCCTTGAGTC
TGGGACTCCCTTTTATCTCTACGGACGTTGGAGGGGCTGAGGAATTATCCCAAGAAGGACGATTTGGACAAATCATTGAG
AGCAATCAAGAGGCAGCTCAGGCGATTACTAATTACATGACTTCTGCCTCAAACTTTGATGTCGATGAGGCTAGCCAATT
CATTCAACAATTTACAATTACAAAACAAATCGAACAAGTAGAAAAACTATTAGAGGAGTAG

Upstream 100 bases:

>100_bases
GATTATATGACGCCTCCACCAGCAGACAAGCAGGTACCGCATCATGATACTGTCATTGCTGATATGGATAAGTCTTATAC
AGAATACAAGGGAGAATATG

Downstream 100 bases:

>100_bases
CATGGAAACTGCATTAATTAGTGTGATTGTGCCAGTCTATAATGTGGCGCAGTACCTAGAAAAATCGATAGCTTCCATTC
AGAAGCAGACCTATCAAAAT

Product: glycosyl transferase, group 1 family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEMEHFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRI
YFPRLARRLLVKDDYDVEVSFTIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNSI
KEVYPDYTSKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVEVIRLLHQEGKNYHLYFIGAGDME
EELKKRVKEYEIEDYVHFLGYQKNPYQYLSQTKVLLSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIE
SNQEAAQAITNYMTSASNFDVDEASQFIQQFTITKQIEQVEKLLEE

Sequences:

>Translated_366_residues
MAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEMEHFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRI
YFPRLARRLLVKDDYDVEVSFTIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNSI
KEVYPDYTSKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVEVIRLLHQEGKNYHLYFIGAGDME
EELKKRVKEYEIEDYVHFLGYQKNPYQYLSQTKVLLSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIE
SNQEAAQAITNYMTSASNFDVDEASQFIQQFTITKQIEQVEKLLEE
>Mature_365_residues
AKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEMEHFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIY
FPRLARRLLVKDDYDVEVSFTIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNSIK
EVYPDYTSKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVEVIRLLHQEGKNYHLYFIGAGDMEE
ELKKRVKEYEIEDYVHFLGYQKNPYQYLSQTKVLLSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIES
NQEAAQAITNYMTSASNFDVDEASQFIQQFTITKQIEQVEKLLEE

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1788357, Length=172, Percent_Identity=25, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI226510984, Length=169, Percent_Identity=26.6272189349112, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 42485; Mature: 42354

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEMEHFDKGYESVPKHVRILK
CCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHCCCHHHHHHHHHHHH
SLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSFTIMNPPLLFSKRREVKKIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHH
WIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNSIKEVYPDYTSKLQTIYNGYDF
HHHHHHHHHHHCCHHHHHHHHHHHHHHHEEECCCHHHHHHHHHCCHHHHHHHHHHCCCCH
QTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVEVIRLLHQEGKNYHLYFIGAGDME
HHHHHCCHHHEEEEECCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCHH
EELKKRVKEYEIEDYVHFLGYQKNPYQYLSQTKVLLSMSKQEGFPGVYVEALSLGLPFIS
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEE
TDVGGAEELSQEGRFGQIIESNQEAAQAITNYMTSASNFDVDEASQFIQQFTITKQIEQV
CCCCCHHHHHHCCCCHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
EKLLEE
HHHHCC
>Mature Secondary Structure 
AKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEMEHFDKGYESVPKHVRILK
CCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHCCCHHHHHHHHHHHH
SLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSFTIMNPPLLFSKRREVKKIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHH
WIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNSIKEVYPDYTSKLQTIYNGYDF
HHHHHHHHHHHCCHHHHHHHHHHHHHHHEEECCCHHHHHHHHHCCHHHHHHHHHHCCCCH
QTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVEVIRLLHQEGKNYHLYFIGAGDME
HHHHHCCHHHEEEEECCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCHH
EELKKRVKEYEIEDYVHFLGYQKNPYQYLSQTKVLLSMSKQEGFPGVYVEALSLGLPFIS
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEE
TDVGGAEELSQEGRFGQIIESNQEAAQAITNYMTSASNFDVDEASQFIQQFTITKQIEQV
CCCCCHHHHHHCCCCHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
EKLLEE
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]