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The map label for this gene is lacG-1 [H]

Identifier: 116516482

GI number: 116516482

Start: 429721

End: 431133

Strand: Direct

Name: lacG-1 [H]

Synonym: SPD_0427

Alternate gene names: 116516482

Gene position: 429721-431133 (Clockwise)

Preceding gene: 116516902

Following gene: 116515623

Centisome position: 21.0

GC content: 35.03

Gene sequence:

>1413_bases
ATGGAAAATTTACAGGTTAAAGCCTTACCGAAGGAGTTTTTATTAGGAACTGCTACCGCTGCTTATCAAGTAGAGGGTGC
AACTAGGGTAGATGGCAAAGGAATAAATATGTGGGATGTTTATTTGCAAGAAAATAGTCCGTTCTTACCAGATCCAGCTA
GTGATTTTTATTATCGTTACGAAGAGGATATAGCTTTGGCGGCAGAACATGGTTTGCAGGCTTTGCGTTTATCTATTTCT
TGGGTTCGTATATTTCCTGATATAGATGGGGATGCTAATGTATTAGCTGTTCATTATTACCATAGAGTTTTTCAGTCTTG
CTTAAAACATAATGTGATTCCGTTTGTTTCTTTACATCATTTTGATTCGCCTCAGAAAATGTTAGAAACAGGGGATTGGT
TGAACAGAGAGAATATTGATCGTTTCATACGATATGCTCGCTTTTGTTTCCAAGAATTTACAGAAGTCAAGCATTGGTTT
ACAATCAATGAACTGATGTCTCTTGCTGCAGGTCAATATATAGGAGGTCAGTTTCCTCCAAATCATCATTTTCAATTATC
TGAAGCAATTCAAGCGAATCATAATATGTTGTTGGCGCATGCTCTTGCAGTCCTCGAATTTCATCAATTAGGGATTGAGG
GAAAGGTAGGTTGTATTCATGCTTTAAAGCCAGGCTATCCTATTGATGGGCAAAAAGAAAATATTTTGGCAGCTAAACGG
TATGATGTTTATAATAATAAATTTCTATTAGATGGAACTTTTTTGGGCTACTACAGTGAGGACACGCTTTTTCACTTGAA
TCAAATATTGGAAGCTAATAATTCTAGCTTTATTATTGAAGATGGTGATTTAGAAATTATGAAGAGAGCTGCACCTCTTA
ATACGATGTTTGTGATGAATTATTATCGTTCAGAATTTATTCGTGAATACAAAGGTGAAAATAGACAAGAATTTAATTCA
ACAGGAATAAAAGGACAGTCTTCTTTTAAATTAAATGCTCTAGGTGAATTTGTAAAAAAACCTGGTATTCCGACAACAGA
TTGGGATTGGAATATTTATCCTCAAGGGTTATTTGATATGTTGCTTCGTATCAAAGAAGAATATCCTCAACATCCGGTCA
TTTATTTAACTGAAAATGGTACAGCCCTTAAAGAAGTTAAGCCAGAGGGCGAGAATGATATTATTGATGACAGTAAGAGA
ATCCGTTATATTGAGCAACATTTACACAAAGTTTTAGAGGCTCGAGATAGAGGAGTCAATATTCAAGGCTATTTTATATG
GTCTTTGCAAGATCAATTTTCTTGGGCGAATGGCTACAATAAGCGATATGGTCTTTTCTTTGTTGATTATGAAACACAGA
AGAGATATATTAAGAAAAGTGCTCTTTGGGTAAAAGGGCTAAAACGGAATTAA

Upstream 100 bases:

>100_bases
GACAACGATGCTATTGTATGATCAGGTAAAGTTTTTTATTGATGAATATGAACGAATTCGAAAGATTGAAGAACATATTG
GTTTGCAATGAGGATTAGTC

Downstream 100 bases:

>100_bases
GGTTAGCGATTTGACTGATGTTTAATATGTTTTAAATATGAGGTTGAATTTTTTATAGGAGGAGTTTTATGGATAAGCTA
GTCGCTGCCATTGAAAAGCA

Product: 6-phospho-beta-galactosidase

Products: NA

Alternate protein names: Beta-D-phosphogalactoside galactohydrolase 1; PGALase 1; P-beta-Gal 1; PBG 1 [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MENLQVKALPKEFLLGTATAAYQVEGATRVDGKGINMWDVYLQENSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSIS
WVRIFPDIDGDANVLAVHYYHRVFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKVGCIHALKPGYPIDGQKENILAAKR
YDVYNNKFLLDGTFLGYYSEDTLFHLNQILEANNSSFIIEDGDLEIMKRAAPLNTMFVMNYYRSEFIREYKGENRQEFNS
TGIKGQSSFKLNALGEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGENDIIDDSKR
IRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDYETQKRYIKKSALWVKGLKRN

Sequences:

>Translated_470_residues
MENLQVKALPKEFLLGTATAAYQVEGATRVDGKGINMWDVYLQENSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSIS
WVRIFPDIDGDANVLAVHYYHRVFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKVGCIHALKPGYPIDGQKENILAAKR
YDVYNNKFLLDGTFLGYYSEDTLFHLNQILEANNSSFIIEDGDLEIMKRAAPLNTMFVMNYYRSEFIREYKGENRQEFNS
TGIKGQSSFKLNALGEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGENDIIDDSKR
IRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDYETQKRYIKKSALWVKGLKRN
>Mature_470_residues
MENLQVKALPKEFLLGTATAAYQVEGATRVDGKGINMWDVYLQENSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSIS
WVRIFPDIDGDANVLAVHYYHRVFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKVGCIHALKPGYPIDGQKENILAAKR
YDVYNNKFLLDGTFLGYYSEDTLFHLNQILEANNSSFIIEDGDLEIMKRAAPLNTMFVMNYYRSEFIREYKGENRQEFNS
TGIKGQSSFKLNALGEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGENDIIDDSKR
IRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDYETQKRYIKKSALWVKGLKRN

Specific function: Can Hydrolyze Salicin, Cellobiose, And Probably Arbutin. [C]

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI13273313, Length=464, Percent_Identity=32.5431034482759, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI32481206, Length=465, Percent_Identity=30.3225806451613, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI110681710, Length=480, Percent_Identity=28.125, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI28376633, Length=199, Percent_Identity=34.6733668341709, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI24497614, Length=217, Percent_Identity=33.6405529953917, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1789070, Length=489, Percent_Identity=31.9018404907975, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI2367174, Length=489, Percent_Identity=31.0838445807771, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI2367270, Length=485, Percent_Identity=32.5773195876289, Blast_Score=183, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17552856, Length=481, Percent_Identity=33.2640332640333, Blast_Score=199, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17539390, Length=485, Percent_Identity=30.5154639175258, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI21356577, Length=470, Percent_Identity=27.0212765957447, Blast_Score=167, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR005928 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.85 [H]

Molecular weight: Translated: 54676; Mature: 54676

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENLQVKALPKEFLLGTATAAYQVEGATRVDGKGINMWDVYLQENSPFLPDPASDFYYRY
CCCCEEEECCHHHHHCCCCEEEEECCCEEECCCCCEEEEEEEECCCCCCCCCCCHHCEEE
EEDIALAAEHGLQALRLSISWVRIFPDIDGDANVLAVHYYHRVFQSCLKHNVIPFVSLHH
CCCHHHHHHHCHHEEEEEEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEEEC
FDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWFTINELMSLAAGQYIGGQFPP
CCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHCCCCCCCCCCC
NHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKVGCIHALKPGYPIDGQKENILAAKR
CCCEEHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCEEEEEE
YDVYNNKFLLDGTFLGYYSEDTLFHLNQILEANNSSFIIEDGDLEIMKRAAPLNTMFVMN
EECCCCEEEEEEEEEEEECCCHHHHHHHHHHCCCCEEEEECCCHHHHHHCCCCHHHHHHH
YYRSEFIREYKGENRQEFNSTGIKGQSSFKLNALGEFVKKPGIPTTDWDWNIYPQGLFDM
HHHHHHHHHHCCCCCHHHHCCCCCCCCCEEHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
LLRIKEEYPQHPVIYLTENGTALKEVKPEGENDIIDDSKRIRYIEQHLHKVLEARDRGVN
HHHHHHHCCCCCEEEEECCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
IQGYFIWSLQDQFSWANGYNKRYGLFFVDYETQKRYIKKSALWVKGLKRN
EEEEEEEEECCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MENLQVKALPKEFLLGTATAAYQVEGATRVDGKGINMWDVYLQENSPFLPDPASDFYYRY
CCCCEEEECCHHHHHCCCCEEEEECCCEEECCCCCEEEEEEEECCCCCCCCCCCHHCEEE
EEDIALAAEHGLQALRLSISWVRIFPDIDGDANVLAVHYYHRVFQSCLKHNVIPFVSLHH
CCCHHHHHHHCHHEEEEEEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEEEC
FDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWFTINELMSLAAGQYIGGQFPP
CCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHCCCCCCCCCCC
NHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKVGCIHALKPGYPIDGQKENILAAKR
CCCEEHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCEEEEEE
YDVYNNKFLLDGTFLGYYSEDTLFHLNQILEANNSSFIIEDGDLEIMKRAAPLNTMFVMN
EECCCCEEEEEEEEEEEECCCHHHHHHHHHHCCCCEEEEECCCHHHHHHCCCCHHHHHHH
YYRSEFIREYKGENRQEFNSTGIKGQSSFKLNALGEFVKKPGIPTTDWDWNIYPQGLFDM
HHHHHHHHHHCCCCCHHHHCCCCCCCCCEEHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
LLRIKEEYPQHPVIYLTENGTALKEVKPEGENDIIDDSKRIRYIEQHLHKVLEARDRGVN
HHHHHHHCCCCCEEEEECCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
IQGYFIWSLQDQFSWANGYNKRYGLFFVDYETQKRYIKKSALWVKGLKRN
EEEEEEEEECCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]