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The map label for this gene is lacE-1 [H]

Identifier: 116515623

GI number: 116515623

Start: 431202

End: 432881

Strand: Direct

Name: lacE-1 [H]

Synonym: SPD_0428

Alternate gene names: 116515623

Gene position: 431202-432881 (Clockwise)

Preceding gene: 116516482

Following gene: 116517130

Centisome position: 21.07

GC content: 35.6

Gene sequence:

>1680_bases
ATGGATAAGCTAGTCGCTGCCATTGAAAAGCAACAAGGGAAATTTGAAAAAATTTCTACTAATAACTATATGATGGCTAT
TAAAGATGGATTCATTGCTACTATGCCTTTAATTATGTTTTCAAGCTTTTTGATGATTATTATTATGATTCCTAAAAATT
TCGGAGTAGAGTTACCGAGTCCAGCTATTGTCTGGATGAGAAAAGTGTATATGTTAACCATGGGAGTTTTGGGTATTATT
GTTTCAGGGACTGTTGGAAAGTCATTAGTTGGAAATGTTAACAGAAAAATGCCTCACGGAAAGGTAATAAATGATATTTC
TGCAATGTTGGCAGCCATATGTAGTTATCTGGTATTAACTGTAACGCTTGTAGTTGATGAGAAGACGGGATCTACAAGTT
TGTCGACAAACTATTTAGGATCTCAAGGATTGATAACTTCGTTTGTCAGTGCCTTTATTACTGTAAATGTTTACCGATTC
TGTATTAAGCGAGACATTACTATTCATTTACCTAAGGAAGTTCCTGGGGCTATATCACAAGCTTTTAGAGATATTTTTCC
TTTTTCTTTTGTTTTACTTATTAGTGGTTTGTTAGATATTGTATCTCGGTTTAGTTTAGATGTTCCTTTTGCCCAAGTAT
TTCAACAACTATTGACTCCTATTTTTAAGGGGGCAGAATCATATCCTGCTATGATGTTGATTTGGTTTATGTGTGCTTTG
CTTTGGTTTGTTGGAATTCATGGACCATCTATTGTCTTACCTGCTGTTACAGCTTTACAACTGAGCAATATGGAAGAGAA
TGCTCAACTTCTTGCAAATGGGCAGTTCCCTTATCATTCTTTAACGCCTAATTTCGGAAATTATATCGCTGCTATTGGAG
GAACGGGGGCTACCTTTGTTGTACCATTTATTTTGATTTTCTTTATGCGGTCTAAACAATTAAAATCGGTAGGTAAAGCT
ACAATTACTCCTGTTTTATTTGCGGTAAATGAACCTCTTCTATTTGGTATGCCTGTTATTTTGAATCCCTATCTTTTTGT
CCCTTTTTTGATGACTCCACCAGTAAATGTATTTCTAGGAAAGGTATTTATTGATTTCTTTGGAATGAATGGATTTTATA
TCCAGTTACCTTGGGCTTTTCCTGGTCCCTTGGGATTGTTAATTGGAACGAATTTTCAACCTATCTCCTTTGTGCTTTTA
TCTTTGATGTTAGTTGTCGACATATTGATTTATTTGCCATTCTGTAGAGCGTATGATAGACAGTTACTGATGAAAGAAGA
TGTTGCAAGCTCAAATGGTATTATTTTAGAGGAGGATACAAGTGAAATAATGCCTGGTGAGATAGATGTAATAAAAAGTA
AGGAGTTGAAAGTACTGGTTCTTTGTGCAGGGTCTGGAACAAGTGCGCAATTAGCCAATGCAATTAACGAGGGCGCTCAA
TTAGCAGAAGTTAGAGTGATTGCTAATTCAGGAGCGTACGGAGCTCATTATGATATTATGGGTGTTTATGATTTAATTAT
TCTGGCCCCACAAGTTCGGAGTTATTATAGAGAGATGAAGGTGGATGCAGAAAGATTAGGTATTCAGATAGTTGCTACCA
GAGGAATGGAATATATTCATTTAACAAAGAATCCAAGTAAATCCTTACAATTTGTATTGGAGCATTACCAAGCTGTGTAG

Upstream 100 bases:

>100_bases
CTCTTTGGGTAAAAGGGCTAAAACGGAATTAAGGTTAGCGATTTGACTGATGTTTAATATGTTTTAAATATGAGGTTGAA
TTTTTTATAGGAGGAGTTTT

Downstream 100 bases:

>100_bases
TAAGTTTTTCCATCTTTTATTTGAGTAAGGACTTTGCTTACAGACAGGCTTGGATTTAAAAACGTTCCCCCTTTTTTTAA
TATAAGAATCCCTCTTTTTA

Product: PTS system, lactose-specific IIBC components

Products: NA

Alternate protein names: EIICB-Lac; EII-Lac; Lactose permease IIC component; PTS system lactose-specific EIIC component; Lactose-specific phosphotransferase enzyme IIB component; PTS system lactose-specific EIIB component [H]

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFLMIIIMIPKNFGVELPSPAIVWMRKVYMLTMGVLGII
VSGTVGKSLVGNVNRKMPHGKVINDISAMLAAICSYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLLTPIFKGAESYPAMMLIWFMCAL
LWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQFPYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKA
TITPVLFAVNEPLLFGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNFQPISFVLL
SLMLVVDILIYLPFCRAYDRQLLMKEDVASSNGIILEEDTSEIMPGEIDVIKSKELKVLVLCAGSGTSAQLANAINEGAQ
LAEVRVIANSGAYGAHYDIMGVYDLIILAPQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKNPSKSLQFVLEHYQAV

Sequences:

>Translated_559_residues
MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFLMIIIMIPKNFGVELPSPAIVWMRKVYMLTMGVLGII
VSGTVGKSLVGNVNRKMPHGKVINDISAMLAAICSYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLLTPIFKGAESYPAMMLIWFMCAL
LWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQFPYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKA
TITPVLFAVNEPLLFGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNFQPISFVLL
SLMLVVDILIYLPFCRAYDRQLLMKEDVASSNGIILEEDTSEIMPGEIDVIKSKELKVLVLCAGSGTSAQLANAINEGAQ
LAEVRVIANSGAYGAHYDIMGVYDLIILAPQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKNPSKSLQFVLEHYQAV
>Mature_559_residues
MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFLMIIIMIPKNFGVELPSPAIVWMRKVYMLTMGVLGII
VSGTVGKSLVGNVNRKMPHGKVINDISAMLAAICSYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLLTPIFKGAESYPAMMLIWFMCAL
LWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQFPYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKA
TITPVLFAVNEPLLFGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNFQPISFVLL
SLMLVVDILIYLPFCRAYDRQLLMKEDVASSNGIILEEDTSEIMPGEIDVIKSKELKVLVLCAGSGTSAQLANAINEGAQ
LAEVRVIANSGAYGAHYDIMGVYDLIILAPQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKNPSKSLQFVLEHYQAV

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1455

COG function: function code G; Phosphotransferase system cellobiose-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=470, Percent_Identity=24.2553191489362, Blast_Score=119, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014350
- InterPro:   IPR003501
- InterPro:   IPR013012
- InterPro:   IPR003352
- InterPro:   IPR004501
- InterPro:   IPR004801 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 61671; Mature: 61671

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS51100 PTS_EIIB_TYPE_3 ; PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFLMIIIMIPKNFGVELPS
CCHHHHHHHHHCCCCEEECCCCEEEEEECCHHHHHHHHHHHHHHHEEEECCCCCCCCCCC
PAIVWMRKVYMLTMGVLGIIVSGTVGKSLVGNVNRKMPHGKVINDISAMLAAICSYLVLT
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRFCIKRDITIHLPKEVPGAISQ
HHHEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHH
AFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLLTPIFKGAESYPAMMLIWFMCAL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQFPYHSLTPNFGNYIAAIGGTGATFV
HHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCEEEEECCCCHHHH
VPFILIFFMRSKQLKSVGKATITPVLFAVNEPLLFGMPVILNPYLFVPFLMTPPVNVFLG
HHHHHHHHHHHHHHHHHCHHHHHHHHEECCCCHHCCCHHHHCCHHHHHHHCCCCHHHHHH
KVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNFQPISFVLLSLMLVVDILIYLPFCRAYDR
HHHHHHHCCCCEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLLMKEDVASSNGIILEEDTSEIMPGEIDVIKSKELKVLVLCAGSGTSAQLANAINEGAQ
HHHHHHHCCCCCCEEEECCCCCCCCCCEEEECCCCEEEEEEECCCCCHHHHHHHHHCCCC
LAEVRVIANSGAYGAHYDIMGVYDLIILAPQVRSYYREMKVDAERLGIQIVATRGMEYIH
EEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEEEECCCEEEE
LTKNPSKSLQFVLEHYQAV
ECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFLMIIIMIPKNFGVELPS
CCHHHHHHHHHCCCCEEECCCCEEEEEECCHHHHHHHHHHHHHHHEEEECCCCCCCCCCC
PAIVWMRKVYMLTMGVLGIIVSGTVGKSLVGNVNRKMPHGKVINDISAMLAAICSYLVLT
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRFCIKRDITIHLPKEVPGAISQ
HHHEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHH
AFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLLTPIFKGAESYPAMMLIWFMCAL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQFPYHSLTPNFGNYIAAIGGTGATFV
HHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCEEEEECCCCHHHH
VPFILIFFMRSKQLKSVGKATITPVLFAVNEPLLFGMPVILNPYLFVPFLMTPPVNVFLG
HHHHHHHHHHHHHHHHHCHHHHHHHHEECCCCHHCCCHHHHCCHHHHHHHCCCCHHHHHH
KVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNFQPISFVLLSLMLVVDILIYLPFCRAYDR
HHHHHHHCCCCEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLLMKEDVASSNGIILEEDTSEIMPGEIDVIKSKELKVLVLCAGSGTSAQLANAINEGAQ
HHHHHHHCCCCCCEEEECCCCCCCCCCEEEECCCCEEEEEEECCCCCHHHHHHHHHCCCC
LAEVRVIANSGAYGAHYDIMGVYDLIILAPQVRSYYREMKVDAERLGIQIVATRGMEYIH
EEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEEEECCCEEEE
LTKNPSKSLQFVLEHYQAV
ECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1400164; 8277252; 12397186 [H]