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The map label for this gene is nanE-2

Identifier: 116516481

GI number: 116516481

Start: 1201026

End: 1201724

Strand: Reverse

Name: nanE-2

Synonym: SPD_1172

Alternate gene names: 116516481

Gene position: 1201724-1201026 (Counterclockwise)

Preceding gene: 116517215

Following gene: 116516633

Centisome position: 58.73

GC content: 43.06

Gene sequence:

>699_bases
ATGTCACAGATTAGCAAAGAAGCCTTGATTGAGCAAATCAAAGATGGAATCATCGTTTCTTGTCAGGCTCTTCCTCATGA
ACCGCTTTATACAGAAGCGGGAGGGGTGATTCCCTTGCTGGTCAAAGCGGCTGAGCAAGGTGGAGCAGTCGGTATCCGAG
CAAACAGTGTTCGCGATATCAAGGAAATTAAGGAAGTCACTAAACTTCCAATCATTGGGATTATCAAACGTGATTATCCA
CCTCAGGAACCCTTCATCACGGCTACTATGAAAGAAGTTGATGAATTGGCAGAACTGGACATCGAGGTGATTGCTCTGGA
TTGTACCAAGCGTGAACGCTACGATGGTTTGGAAATTCAAGAGTTCATTCGTCAGGTTAAGGAGAAATATCCTAATCAGC
TTTTGATGGCTGATACTAGTATCTTCGAAGAAGGGCTAGCAGCTGTAGAAGCAGGAATTGACTTTGTCGGAACAACCTTA
TCAGGCTACACATCCTACAGTCCAAAAGTAGACGGTCCAGATTTTGAATTGATTAAGAAACTCTGTGATGCCGGTGTAGA
TGTCATTGCAGAAGGAAAAATTTATACACCAGAACAAGCCAAACAAATCCTTGAATATGGAGTGCGAGGCATCGTTGTTG
GTGGCGCTATTACTAGACCAAAAGAGATTACAGAACGTTTTGTAGCGGGATTAAAATAG

Upstream 100 bases:

>100_bases
GGATTACGAATGAATTTGATTGCTGATATTATCAATCATGAACTAGGATTCTAGTTTTGCAGGAAGTCTAATAAATGTAT
AAGAAAAAGGAGGAAAAAGG

Downstream 100 bases:

>100_bases
CATGCCAGAGTAAGAGGTGTAATATGAAAATTGATGTTATAAATCATTTAGACTCACCAGGATTAGCTGGGATGATTTGT
AGACGGATTAATAATAGAAT

Product: N-acetylmannosamine-6-phosphate 2-epimerase

Products: NA

Alternate protein names: ManNAc-6-P epimerase 1

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MSQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAAEQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYP
PQEPFITATMKEVDELAELDIEVIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIYTPEQAKQILEYGVRGIVVGGAITRPKEITERFVAGLK

Sequences:

>Translated_232_residues
MSQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAAEQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYP
PQEPFITATMKEVDELAELDIEVIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIYTPEQAKQILEYGVRGIVVGGAITRPKEITERFVAGLK
>Mature_231_residues
SQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAAEQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPP
QEPFITATMKEVDELAELDIEVIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTLS
GYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIYTPEQAKQILEYGVRGIVVGGAITRPKEITERFVAGLK

Specific function: Converts N-acetylmannosamine-6-phosphate (ManNAc-6-P) to N-acetylglucosamine-6-phosphate (GlcNAc-6-P) (Potential)

COG id: COG3010

COG function: function code G; Putative N-acetylmannosamine-6-phosphate epimerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nanE family

Homologues:

Organism=Escherichia coli, GI1789617, Length=218, Percent_Identity=38.9908256880734, Blast_Score=132, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NANE1_STRR6 (Q8DPF0)

Other databases:

- EMBL:   AE007317
- PIR:   C98021
- RefSeq:   NP_358789.1
- ProteinModelPortal:   Q8DPF0
- SMR:   Q8DPF0
- STRING:   Q8DPF0
- EnsemblBacteria:   EBSTRT00000016201
- GeneID:   934409
- GenomeReviews:   AE007317_GR
- KEGG:   spr:spr1196
- eggNOG:   COG3010
- GeneTree:   EBGT00050000028071
- HOGENOM:   HBG296941
- OMA:   RVEGIEN
- PhylomeDB:   Q8DPF0
- ProtClustDB:   PRK01130
- BioCyc:   SPNE171101:SPR1196-MONOMER
- HAMAP:   MF_01235
- InterPro:   IPR013785
- InterPro:   IPR007260
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF04131 NanE; SSF51366 RibP_bind_barrel

EC number: =5.1.3.9

Molecular weight: Translated: 25437; Mature: 25305

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAAEQGGAVGIRANSVRDI
CCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCHHHH
KEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIEVIALDCTKRERYDGLEIQ
HHHHHHHHCCHHEEEECCCCCCCCEEHHHHHHHHHHHHCCEEEEEEECCCHHCCCCCHHH
EFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTLSGYTSYSPKVDGPDFELIKK
HHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCHHHHHH
LCDAGVDVIAEGKIYTPEQAKQILEYGVRGIVVGGAITRPKEITERFVAGLK
HHHCCCCEEECCCEECHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAAEQGGAVGIRANSVRDI
CCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCHHHH
KEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIEVIALDCTKRERYDGLEIQ
HHHHHHHHCCHHEEEECCCCCCCCEEHHHHHHHHHHHHCCEEEEEEECCCHHCCCCCHHH
EFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTLSGYTSYSPKVDGPDFELIKK
HHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCHHHHHH
LCDAGVDVIAEGKIYTPEQAKQILEYGVRGIVVGGAITRPKEITERFVAGLK
HHHCCCCEEECCCEECHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11544234