Click here to switch to the map view.

The map label for this gene is rafE [H]

Identifier: 116516419

GI number: 116516419

Start: 1692049

End: 1693308

Strand: Reverse

Name: rafE [H]

Synonym: SPD_1677

Alternate gene names: 116516419

Gene position: 1693308-1692049 (Counterclockwise)

Preceding gene: 116515396

Following gene: 116517088

Centisome position: 82.76

GC content: 43.17

Gene sequence:

>1260_bases
ATGGAATGGTATAAAAAAATCGGACTTCTTGCAACTACAGGTTTAGCTTTGTTTGGGCTCGGCGCTTGCTCCAACTATGG
TAAATCTGCGGATGGCACAGTGACCATCGAGTATTTCAACCAGAAAAAAGAAATGACCAAAACCTTGGAAGAAATCACTC
GTGATTTTGAGAAGGAAAACCCTAAGATCAAGGTCAAAGTCGTCAATGTACCAAATGCTGGTGAAGTATTGAAGACACGC
GTTCTCGCAGGAGATGTGCCTGATGTGGTCAATATTTACCCACAGTCCATCGAACTGCAAGAATGGGCAAAAGCAGGTGT
TTTTGAAGATTTGAGCAACAAAGACTACCTGAAACGCGTGAAAAATGGCTACGCTGAAAAATATGCTGTAAACGAAAAAG
TTTACAACGTTCCTTTTACAGCTAATGCTTATGGAATTTACTACAACAAAGATAAATTCGAAGAACTGGGCTTGAAGGTT
CCTGAAACCTGGGATGAATTTGAACAGTTAGTCAAAGATATCGTTGCTAAAGGACAAACACCATTTGGAATTGCAGGTGC
AGATGCTTGGACACTCAATGGTTACAATCAATTAGCCTTTGCGACAGCAACAGGTGGAGGAAAAGAAGCAAATCAATACC
TTCGTTATTCTCAACCAAATGCCATTAAATTGTCGGATCCGATTATGAAAGATGATATCAAGGTCATGGACATCCTTCGC
ATCAATGGCTCTAAGCAAAAGAACTGGGAAGGTGCTGGCTATACCGATGTTATCGGAGCCTTCGCACGTGGGGATGTCCT
CATGACACCAAATGGGTCTTGGGCGATCACAGCGATTAATGAACAAAAACCGAACTTTAAGATTGGGACCTTCATGATTC
CAGGAAAAGAAAAAGGACAAAGCTTAACCGTTGGTGCGGGAGACTTGGCATGGTCTATCTCAGCCACCACCAAACATCCA
AAAGAAGCCAATGCCTTTGTGGAATATATGACCCGTCCAGAAGTCATGCAAAAATACTACGATGTGGACGGATCTCCAAC
AGCGATCGAAGGGGTCAAACAAGCAGGAGAAGATTCACCGCTTGCTGGTATGACCGAATATGCCTTTACGGATCGTCACT
TGGTCTGGTTGCAACAATACTGGACCAGTGAAGCAGACTTCCATACCTTGACCATGAACTATGTCTTGACCGGTGATAAA
GAAGGCATGGTCAATGATTTGAATGCCTTCTTTAACCCGATGAAAGCGGATGTGGATTAG

Upstream 100 bases:

>100_bases
AAACTATGATATGTACTGACCCCAAAAGTTAGATTTATTCTGTCTAACTTTTGGGGTGCAGTTCAATAAGGGTGTATAAA
TTTATAAAGGAGGCTTTCTA

Downstream 100 bases:

>100_bases
GAGTAGAGAGACTATGAAAAAAGTATTACAAAAATATTGGGCATGGGCTTTTGTGGTCATCCCCCTCTTGTTACAAGCAA
TTTTCTTCTATGTGCCGATG

Product: sugar ABC transporter sugar-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKEMTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTR
VLAGDVPDVVNIYPQSIELQEWAKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQPNAIKLSDPIMKDDIKVMDILR
INGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAITAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHP
KEANAFVEYMTRPEVMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTMNYVLTGDK
EGMVNDLNAFFNPMKADVD

Sequences:

>Translated_419_residues
MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKEMTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTR
VLAGDVPDVVNIYPQSIELQEWAKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQPNAIKLSDPIMKDDIKVMDILR
INGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAITAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHP
KEANAFVEYMTRPEVMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTMNYVLTGDK
EGMVNDLNAFFNPMKADVD
>Mature_419_residues
MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKEMTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTR
VLAGDVPDVVNIYPQSIELQEWAKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQPNAIKLSDPIMKDDIKVMDILR
INGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAITAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHP
KEANAFVEYMTRPEVMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTMNYVLTGDK
EGMVNDLNAFFNPMKADVD

Specific function: Involved in a binding protein-dependent transport system responsible for the uptake of melibiose, raffinose and isomaltotriose [H]

COG id: COG1653

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR006061 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 46662; Mature: 46662

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01037 SBP_BACTERIAL_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKEMTKTLEEITRDFEKEN
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCC
PKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEWAKAGVFEDLSNKDYLKRV
CEEEEEEEECCCCCHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHCCCHHHCCCHHHHHHH
KNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKVPETWDEFEQLVKDIVAKGQT
HCCHHHHEEECCEEEECCEECCEEEEEECCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCC
PFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQPNAIKLSDPIMKDDIKVMDILR
CCEECCCCEEEECCCCEEEEEEECCCCHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEEE
INGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAITAINEQKPNFKIGTFMIPGKEKGQ
ECCCCCCCCCCCCHHHHHHHHHCCCEEECCCCCEEEEEECCCCCCEEEEEEEECCCCCCC
SLTVGAGDLAWSISATTKHPKEANAFVEYMTRPEVMQKYYDVDGSPTAIEGVKQAGEDSP
EEEEECCCEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHCCCCCC
LAGMTEYAFTDRHLVWLQQYWTSEADFHTLTMNYVLTGDKEGMVNDLNAFFNPMKADVD
CCCCHHHHHCCCHHEEHHHHHCCCCCEEEEEEEEEEECCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKEMTKTLEEITRDFEKEN
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCC
PKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEWAKAGVFEDLSNKDYLKRV
CEEEEEEEECCCCCHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHCCCHHHCCCHHHHHHH
KNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKVPETWDEFEQLVKDIVAKGQT
HCCHHHHEEECCEEEECCEECCEEEEEECCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCC
PFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQPNAIKLSDPIMKDDIKVMDILR
CCEECCCCEEEECCCCEEEEEEECCCCHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEEE
INGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAITAINEQKPNFKIGTFMIPGKEKGQ
ECCCCCCCCCCCCHHHHHHHHHCCCEEECCCCCEEEEEECCCCCCEEEEEEEECCCCCCC
SLTVGAGDLAWSISATTKHPKEANAFVEYMTRPEVMQKYYDVDGSPTAIEGVKQAGEDSP
EEEEECCCEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHCCCCCC
LAGMTEYAFTDRHLVWLQQYWTSEADFHTLTMNYVLTGDKEGMVNDLNAFFNPMKADVD
CCCCHHHHHCCCHHEEHHHHHCCCCCEEEEEEEEEEECCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1537846; 12397186 [H]