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The map label for this gene is rafF [H]

Identifier: 116517088

GI number: 116517088

Start: 1691169

End: 1692035

Strand: Reverse

Name: rafF [H]

Synonym: SPD_1676

Alternate gene names: 116517088

Gene position: 1692035-1691169 (Counterclockwise)

Preceding gene: 116516419

Following gene: 116516219

Centisome position: 82.7

GC content: 41.98

Gene sequence:

>867_bases
ATGAAAAAAGTATTACAAAAATATTGGGCATGGGCTTTTGTGGTCATCCCCCTCTTGTTACAAGCAATTTTCTTCTATGT
GCCGATGTTTCAAGGAGCCTTTTACAGTTTTACCAACTGGACAGGATTGACTTATAACTACAAATTTGTCGGCTTAAACA
ACTTTAAGCTCCTCTTCATGGATCCAAAATTCATGAATGCGATTGGCTTTACCGCAATCATTACGATTGCCATGGTGGTT
GGTGAGATTGCACTCGGGATCTTCATTGCGCGTGTCTTGAACTCTAAAATCAAAGGCCAAACCTTCTTCCGTTCTTGGTT
CTTCTTCCCAGCTGTTTTATCTGGTTTGACAGTGGCTTTGATCTTCAAGCAAGTCTTCAACTACGGTCTTCCAGCGATTG
GGAATGCCCTTCATATTGAATTTCTCCAAACCAGTCTTTTAGGGACTAAGTGGGGAGCAATCTTTGCGGCTGTCTTTGTC
CTTCTTTGGCAAGGGGTGGCTATGCCCATCATCATCTTCCTAGCTGGTTTGCAATCTATTCCAACTGAGATTACAGAGGC
AGCAAGGATTGATGGTGCGACTAGCAAGCAAGTTTTCTGGAACATTGAATTGCCTTACTTGCTACCAAGTGTCTCTATAG
TCTTTATCCTAGCCCTAAAAGGTGGGCTGACTGCCTTTGACCAAGTCTTTGCCATGACCGGTGGTGGTCCAAACAATGCC
ACAACCTCACTTGGGCTCTTGGTTTATAACTATGCCTTTAAAAACAACCAATTCGGTTATGCCAATGCCATTGCCGTAAT
CTTGTTCTTCTTAATTGTAGTGATTTCGATCATCCAATTGAGAGTATCTAAGAAATTTGAAATTTAA

Upstream 100 bases:

>100_bases
ATGAACTATGTCTTGACCGGTGATAAAGAAGGCATGGTCAATGATTTGAATGCCTTCTTTAACCCGATGAAAGCGGATGT
GGATTAGGAGTAGAGAGACT

Downstream 100 bases:

>100_bases
GAGGAGAAGCATGATGAAACAAGATGAAAGAAAAGCCCTGATTGGCAAATACATTCTATTGATTCTAGGATCGGTTCTGA
TTTTAGTGCCGCTCCTTGCT

Product: sugar ABC transporter permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLTYNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVV
GEIALGIFIARVLNSKIKGQTFFRSWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILALKGGLTAFDQVFAMTGGGPNNA
TTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVISIIQLRVSKKFEI

Sequences:

>Translated_288_residues
MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLTYNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVV
GEIALGIFIARVLNSKIKGQTFFRSWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILALKGGLTAFDQVFAMTGGGPNNA
TTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVISIIQLRVSKKFEI
>Mature_288_residues
MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLTYNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVV
GEIALGIFIARVLNSKIKGQTFFRSWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILALKGGLTAFDQVFAMTGGGPNNA
TTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVISIIQLRVSKKFEI

Specific function: Involved in a binding protein-dependent transport system responsible for the uptake of melibiose, raffinose and isomaltotriose [H]

COG id: COG1175

COG function: function code G; ABC-type sugar transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789861, Length=292, Percent_Identity=28.7671232876712, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1787570, Length=241, Percent_Identity=29.8755186721992, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790465, Length=256, Percent_Identity=25.78125, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32108; Mature: 32108

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLTYNYKFVGLNNFKLLFM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEEEEEECCEEEEEE
DPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTFFRSWFFFPAVLSGLTVAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
IFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFVLLWQGVAMPIIIFLAGLQSI
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
PTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILALKGGLTAFDQVFAMTGGGPNNA
CHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCC
TTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVISIIQLRVSKKFEI
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLTYNYKFVGLNNFKLLFM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEEEEEECCEEEEEE
DPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTFFRSWFFFPAVLSGLTVAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
IFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFVLLWQGVAMPIIIFLAGLQSI
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
PTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILALKGGLTAFDQVFAMTGGGPNNA
CHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCC
TTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVISIIQLRVSKKFEI
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1537846; 12397186 [H]