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The map label for this gene is lysA [H]

Identifier: 116516303

GI number: 116516303

Start: 1771044

End: 1772294

Strand: Reverse

Name: lysA [H]

Synonym: SPD_1775

Alternate gene names: 116516303

Gene position: 1772294-1771044 (Counterclockwise)

Preceding gene: 116515481

Following gene: 116516256

Centisome position: 86.62

GC content: 45.16

Gene sequence:

>1251_bases
ATGAAAACACCATTTATCAATAGAGAAGAGTTAGAAGCGATTGTTGCCGAGTTCCCGACTCCCTTTCACTTGTATGATGA
GAAGGGGATTCGTGAGAAGGCAAGAGCTGTCAACCAAGCTTTTTCGTGGAACAAGGGCTTTAAGGAATATTTTGCAGTTA
AGGCTACTCCAACTCCAGCTATTTTGAAAATTCTCCAAGAAGAAGGTTGTGGTGTGGACTGCTCTAGTTATGTAGAGCTT
TTGATGAGCCATAAACTGGACTTTCTGGGTTCTGAGATTATGTTCTCTTCCAACAACACGCCAGACAAGGAATACGCCTA
TGCACGTGAATTGGGTGCGACCATTAACTTGGATGCCTTTGAAGATATTGAACATCTGGAGAGAGTAGCAGGCATTCCAG
AAATCATCTCTTGTCGTTATAATCCTGGAGGCGTTTTTGAACTGGGGACAGACATTATGGACAATCCTGGGGAGGCTAAG
TTTGGCATGACCAAGGACCAGCTCTTTGAAGCCTTTGCTATCTTGAAGGAAAAAGGAGCCAAGACTTTTGGGCTTCACTC
CTTCCTAGCGTCCAATACCGTGACCCATCTCTATTATCCAGAGTTGGCTCGTCAGCTCTTTGAACTGGCTGTTGAAATCA
AGGAAAAGTTGGGCATTTGGCTAGACTTTATCAATCTTTCTGGCGGTATTGGTGTTAATTATCGTCCAGACCAGGAGCCG
AACGATATCGCCTTGATTGGTGAGGGAGTTCGTAAGGTGTATGAAGAAGTTCTTACGTCAGCAGGTCTTGGTCAGGTCAA
GATTTTCACCGAATTGGGTCGTTTTATGCTGGCACCTCACGGTGCTCTAGTCACAAGAGTCACTCATAAGAAGGAAACCT
ACCGTACCTATCTAGGTGTGGATGCCTCAGCAGTCAACCTCATGCGTCCAGCTATGTACGGAGCTTACCATCATATTAGC
AACGTGACCCATCCAGATGGACCAGCTGAAGCGGTAGATGTGGTTGGTTCACTCTGTGAAAACAATGATAAATTTGCAGT
CAATCGCGAACTGCCTCATACAGAAATCGGTGATTTGCTGGTAATTCATGATACAGGTGCCCACGGATTTTCAATGGGCT
ACCAGTATAATGCCAAATTACGTTCTGCGGAAATCCTCTATACCGAAGAAGGTAAAGCCCGTCAAATCCGCCGTGCAGAG
CGCCCCGAGGACTATTTTGCAACCTTATATGGCTTTGATTTTGAAGAATAA

Upstream 100 bases:

>100_bases
TGAGGTTGGCAATATCTTTGACGAAGATAAATAAGAGATAGAACTAAAGGTTGGAACGATTGTCCCAGCCTTTCTTTGCA
AACAGAATAGAAGGAAGCTT

Downstream 100 bases:

>100_bases
TCTGATGATAGATTGAAAATGAAATTGAAAAACAGATTGCTTTCTAAAAAATAGGCAAAAATCTTGTTTTTCCTTCAAGT
CGTGATATAATAAAACTATA

Product: diaminopimelate decarboxylase

Products: L-lysine; CO2

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWNKGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVEL
LMSHKLDFLGSEIMFSSNNTPDKEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
FGMTKDQLFEAFAILKEKGAKTFGLHSFLASNTVTHLYYPELARQLFELAVEIKEKLGIWLDFINLSGGIGVNYRPDQEP
NDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFMLAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHIS
NVTHPDGPAEAVDVVGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGKARQIRRAE
RPEDYFATLYGFDFEE

Sequences:

>Translated_416_residues
MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWNKGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVEL
LMSHKLDFLGSEIMFSSNNTPDKEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
FGMTKDQLFEAFAILKEKGAKTFGLHSFLASNTVTHLYYPELARQLFELAVEIKEKLGIWLDFINLSGGIGVNYRPDQEP
NDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFMLAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHIS
NVTHPDGPAEAVDVVGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGKARQIRRAE
RPEDYFATLYGFDFEE
>Mature_416_residues
MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWNKGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVEL
LMSHKLDFLGSEIMFSSNNTPDKEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
FGMTKDQLFEAFAILKEKGAKTFGLHSFLASNTVTHLYYPELARQLFELAVEIKEKLGIWLDFINLSGGIGVNYRPDQEP
NDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFMLAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHIS
NVTHPDGPAEAVDVVGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGKARQIRRAE
RPEDYFATLYGFDFEE

Specific function: Involved in tabtoxin production and pathogenicity [H]

COG id: COG0019

COG function: function code E; Diaminopimelate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Orn/Lys/Arg decarboxylase class-II family [H]

Homologues:

Organism=Escherichia coli, GI1789203, Length=415, Percent_Identity=29.8795180722892, Blast_Score=138, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009006
- InterPro:   IPR002986
- InterPro:   IPR022643
- InterPro:   IPR022657
- InterPro:   IPR022644
- InterPro:   IPR022653
- InterPro:   IPR000183 [H]

Pfam domain/function: PF02784 Orn_Arg_deC_N; PF00278 Orn_DAP_Arg_deC [H]

EC number: 4.1.1.20

Molecular weight: Translated: 46649; Mature: 46649

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWNKGFKEYFAVKATPTPA
CCCCCCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHCCCCCHHHEEEECCCCHH
ILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPDKEYAYARELGATINLDAF
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCEECCHHH
EDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAKFGMTKDQLFEAFAILKEKGA
HHHHHHHHHHCCCHHHEEECCCCCCEEECCHHHCCCCCCCCCCCHHHHHHHHHHHHHCCC
KTFGLHSFLASNTVTHLYYPELARQLFELAVEIKEKLGIWLDFINLSGGIGVNYRPDQEP
CHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCCC
NDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFMLAPHGALVTRVTHKKETYRTYLGV
CCEEEECHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
DASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDVVGSLCENNDKFAVNRELPHTEIGDLL
CHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEE
VIHDTGAHGFSMGYQYNAKLRSAEILYTEEGKARQIRRAERPEDYFATLYGFDFEE
EEECCCCCCCCCCEEECCCCCCEEEEEECCCHHHHHHHHCCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWNKGFKEYFAVKATPTPA
CCCCCCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHCCCCCHHHEEEECCCCHH
ILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPDKEYAYARELGATINLDAF
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCEECCHHH
EDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAKFGMTKDQLFEAFAILKEKGA
HHHHHHHHHHCCCHHHEEECCCCCCEEECCHHHCCCCCCCCCCCHHHHHHHHHHHHHCCC
KTFGLHSFLASNTVTHLYYPELARQLFELAVEIKEKLGIWLDFINLSGGIGVNYRPDQEP
CHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCCC
NDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFMLAPHGALVTRVTHKKETYRTYLGV
CCEEEECHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
DASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDVVGSLCENNDKFAVNRELPHTEIGDLL
CHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEE
VIHDTGAHGFSMGYQYNAKLRSAEILYTEEGKARQIRRAERPEDYFATLYGFDFEE
EEECCCCCCCCCCEEECCCCCCEEEEEECCCHHHHHHHHCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: D-Lysine; Pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1.7 {meso-2,6-diamininoheptanedioate}} [C]

Substrates: meso-2,6-diaminoheptanedioate

Specific reaction: meso-2,6-diaminoheptanedioate = L-lysine + CO2

General reaction: Decarboxylation [C]

Inhibitor: 2, 3-Diaminopropionic acid; Glutamic acid; L-Cystine; L-Lysine; Penicillamine [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1515668 [H]