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The map label for this gene is pflA [H]

Identifier: 116516256

GI number: 116516256

Start: 1770124

End: 1770918

Strand: Reverse

Name: pflA [H]

Synonym: SPD_1774

Alternate gene names: 116516256

Gene position: 1770918-1770124 (Counterclockwise)

Preceding gene: 116516303

Following gene: 116516909

Centisome position: 86.55

GC content: 44.15

Gene sequence:

>795_bases
ATGTCTGAAGAAACAATTGATTATGGACAAGTGACAGGGATGGTGCATTCGACAGAAAGCTTTGGGTCAGTAGATGGGCC
TGGTATTCGCTTTATTGTCTTTTTGCAGGGCTGTCACATGCGTTGCCAGTATTGCCACAACCCAGACACTTGGGCTATGG
AGTCCAATAAGTCACGTGAACGGACGGTAGATGATGTCTTGACAGAGGCCTTGCGCTACCGTGGTTTCTGGGGAAATAAG
GGTGGGATTACAGTCAGTGGAGGAGAAGCTCTCTTGCAGATTGATTTCCTGATTGCTCTCTTCACCAAGGCTAAGGAACA
AGGAATCCACTGTACCTTGGACACCTGTGCTCTTCCTTTCCGTAATAAACCGCGTTACCTTGAGAAGTTTGACAAACTCA
TGGCTGTCACTGACTTGGTTCTTTTGGATATCAAGGAAATCAACGAAGAACAGCACAAGATTGTCACTAGCCAAACCAAT
AAAAATATCTTGGCTTGTGCCCAGTATCTATCAGATATTGGAAAACCTGTCTGGATTCGCCACGTGCTAGTTCCAGGATT
GACAGACAGAGATGATGACTTGATTGAACTTGGTAAGTTCGTCAAGACCCTCAAAAATGTTGATAAGTTTGAAATTCTAC
CTTATCACACCATGGGTGAGTTCAAGTGGCGTGAACTTGGAATTCCATATTCCCTCGAAGGAGTCAAACCACCAACAGCA
GATCGCGTCAAGAACGCTAAAAAACTCATGGATACCGAAAGTTATCAAGATTATATGAAACGTGTACATGGATAG

Upstream 100 bases:

>100_bases
TGAAAAACAGATTGCTTTCTAAAAAATAGGCAAAAATCTTGTTTTTCCTTCAAGTCGTGATATAATAAAACTATAAAACG
TTTTCAAGGAAGGTAACGAT

Downstream 100 bases:

>100_bases
AAAAGAAGCCTGATGGAAACATCGGGCTTTTGACTTGCAAAAAGACTTAGCAAATCAGCTAAGCCTTTTTCTTCTTATCT
CGAACGTTGTTTTCCAGCGT

Product: pyruvate formate-lyase activating enzyme

Products: NA

Alternate protein names: PFL-activating enzyme; Formate-C-acetyltransferase-activating enzyme [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQYCHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNK
GGITVSGGEALLQIDFLIALFTKAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTMGEFKWRELGIPYSLEGVKPPTA
DRVKNAKKLMDTESYQDYMKRVHG

Sequences:

>Translated_264_residues
MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQYCHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNK
GGITVSGGEALLQIDFLIALFTKAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTMGEFKWRELGIPYSLEGVKPPTA
DRVKNAKKLMDTESYQDYMKRVHG
>Mature_263_residues
SEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQYCHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKG
GITVSGGEALLQIDFLIALFTKAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTNK
NILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTMGEFKWRELGIPYSLEGVKPPTAD
RVKNAKKLMDTESYQDYMKRVHG

Specific function: Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=248, Percent_Identity=44.758064516129, Blast_Score=225, Evalue=3e-60,
Organism=Escherichia coli, GI1790839, Length=294, Percent_Identity=26.8707482993197, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1790389, Length=271, Percent_Identity=25.0922509225092, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI226510931, Length=275, Percent_Identity=23.2727272727273, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 30125; Mature: 29994

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQYCHNPDTWAMESNKSRE
CCCCCCCHHHHHCEEECCCCCCCCCCCCEEEEEEEECCCHHHHCCCCCCCCCCCCCCCHH
RTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFTKAKEQGIHCTLDTCALPF
HHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEHHHCCCC
RNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTNKNILACAQYLSDIGKPVWIR
CCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHEECCCCCCHHHHHHHHHHHCCCCEEEH
HVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTMGEFKWRELGIPYSLEGVKPPTA
HHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHCCCCEEECCCCCCCH
DRVKNAKKLMDTESYQDYMKRVHG
HHHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure 
SEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQYCHNPDTWAMESNKSRE
CCCCCCHHHHHCEEECCCCCCCCCCCCEEEEEEEECCCHHHHCCCCCCCCCCCCCCCHH
RTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFTKAKEQGIHCTLDTCALPF
HHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEHHHCCCC
RNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTNKNILACAQYLSDIGKPVWIR
CCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHEECCCCCCHHHHHHHHHHHCCCCEEEH
HVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTMGEFKWRELGIPYSLEGVKPPTA
HHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHCCCCEEECCCCCCCH
DRVKNAKKLMDTESYQDYMKRVHG
HHHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11029425; 10899886; 12397186 [H]