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The map label for this gene is phoB [C]

Identifier: 116516299

GI number: 116516299

Start: 1999166

End: 1999819

Strand: Reverse

Name: phoB [C]

Synonym: SPD_2020

Alternate gene names: 116516299

Gene position: 1999819-1999166 (Counterclockwise)

Preceding gene: 116515451

Following gene: 116515721

Centisome position: 97.74

GC content: 36.09

Gene sequence:

>654_bases
ATGAACATTTTAGTTGCAGATGACGAGGAAATGATTAGAGAAGGAATTGCAGCATTTCTGACAGAAGAGGGTTATCATGT
CATTATGGCTAAGGATGGACAAGAGGTCTTGGAAAAATTTCAAGATCTCCCTATCCATCTCATGGTATTGGATTTAATGA
TGCCTAGGAAGAGTGGTTTTGAAGTGTTAAAAGAAATCAATCAAAAGCACGATATTCCTGTCATCGTCTTGAGTGCTCTG
GGAGATGAAACTACTCAGTCACAGGTATTTGATCTCTATGCTGATGATCATGTGACAAAACCTTTTTCTTTGGTACTGCT
TGTCAAGCGTATTAAGGCGCTTATCAGACGTTACTACGTCATAGAGGATATTTGGCGATATCAGGATGTAACAGTGGATT
TTACCTCTTACAAAGCACATTATAAAAATGAAGAAATTGATCTCAAACCAAAGGAATTACTGGTACTAAAGTGTTTGATT
CAGCATAAAAATCAAGTTTTAAGTAGAGAGCAGATATTGGAAGAAATTTCAAAAGATGTAGCTGATTTACCTTGTGATAG
GGTCGTTGATGTCTATATTCGTACTCTTCGCAAAAAATTAGCTTTAGATTGTATCGTGACTGTGAAAAATGTTGGGTATA
AGATTAGCTTATGA

Upstream 100 bases:

>100_bases
GAAATCCATTCCATTATCATAGATGATAGGCTGGTAGGAAATTTTCAAATAGCATACAGGAAATAGATGTATGGAGTTCT
GGTAGTAGAAAGGGAGAGAG

Downstream 100 bases:

>100_bases
TAAAAAATCCTAAATTATTAACCAAGTCTTTTTTAAGAAGTTTTGCAATTCTAGGTGGTGTTGGTCTAGTCATTCATATA
GCTATTTATTTGACCTTTCC

Product: DNA-binding response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLPIHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSAL
GDETTQSQVFDLYADDHVTKPFSLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL

Sequences:

>Translated_217_residues
MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLPIHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSAL
GDETTQSQVFDLYADDHVTKPFSLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL
>Mature_217_residues
MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLPIHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSAL
GDETTQSQVFDLYADDHVTKPFSLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL

Specific function: This Protein Is A Positive Regulator For The Phosphate Regulon. Transcription Of This Operon Is Positively Regulated By Phob And Phor When Phosphate Is Limited. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=223, Percent_Identity=34.0807174887892, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1786911, Length=224, Percent_Identity=29.9107142857143, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789402, Length=218, Percent_Identity=31.1926605504587, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1788394, Length=222, Percent_Identity=30.6306306306306, Blast_Score=104, Evalue=4e-24,
Organism=Escherichia coli, GI2367329, Length=234, Percent_Identity=34.1880341880342, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1787229, Length=220, Percent_Identity=27.2727272727273, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1786784, Length=223, Percent_Identity=30.0448430493274, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1790860, Length=223, Percent_Identity=29.1479820627803, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1789809, Length=225, Percent_Identity=28.4444444444444, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87082012, Length=218, Percent_Identity=27.9816513761468, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1790552, Length=218, Percent_Identity=30.7339449541284, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1790863, Length=229, Percent_Identity=24.0174672489083, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=25.5605381165919, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI145693140, Length=229, Percent_Identity=26.2008733624454, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25205; Mature: 25205

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLPIHLMVLDLMMPRKSGF
CCEEECCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHCCCCCHH
EVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPFSLVLLVKRIKALIRRYYV
HHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
IEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLIQHKNQVLSREQILEEISKDV
HHHHHHHHCEEEEHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECC
>Mature Secondary Structure
MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLPIHLMVLDLMMPRKSGF
CCEEECCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHCCCCCHH
EVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPFSLVLLVKRIKALIRRYYV
HHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
IEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLIQHKNQVLSREQILEEISKDV
HHHHHHHHCEEEEHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1554697 [H]