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The map label for this gene is vanSB [H]

Identifier: 116515721

GI number: 116515721

Start: 1997838

End: 1999169

Strand: Reverse

Name: vanSB [H]

Synonym: SPD_2019

Alternate gene names: 116515721

Gene position: 1999169-1997838 (Counterclockwise)

Preceding gene: 116516299

Following gene: 116516618

Centisome position: 97.71

GC content: 34.38

Gene sequence:

>1332_bases
ATGATAAAAAATCCTAAATTATTAACCAAGTCTTTTTTAAGAAGTTTTGCAATTCTAGGTGGTGTTGGTCTAGTCATTCA
TATAGCTATTTATTTGACCTTTCCTTTTTATTATATTCAACTGGAGGGGGAAAAGTTTAATGAGAGCGCAAGAGTGTTTA
CGGAGTATTTAAAGACTAAGACATCTGATGAAATTCCAAGCTTACTCCAGTCTTATTCAAAGTCCTTGACCATATCTGCT
CACCTTAAAAGAGATATTGTAGATAAGCGGCTCCCTCTTGTGCATGACTTGGATATTAAAGATGGAAAGCTATCAAATTA
TATCGTGATGTTAGATATGTCTGTTAGTACAGCAGATGGTAAACAGGTAACAGTGCAATTTGTTCACGGGGTGGATGTCT
ACAAAGAAGCAAAGAATATTTTGCTTTTGTATCTCCCATATACATTTTTGGTTACAATTGCTTTTTCCTTTGTTTTTTCT
TATTTTTATACTAAACGCTTGCTCAATCCTCTTTTTTACATTTCAGAAGTGACTAGTAAAATGCAAGATTTGGATGACAA
TATTCGTTTTGATGAAAGTAGGAAAGATGAAGTTGGTGAAGTTGGAAAACAGATTAATGGTATGTATGAGCACTTGTTGA
AGGTTATTCATGAGTTGGAAAGTCGTAATGAGCAAATTGTAAAATTGCAAAATCAAAAGGTTTCCTTTGTCCGCGGAGCA
TCACATGAGTTGAAAACCCCTTTAGCCAGTCTTAGAATTATCCTAGAGAATATGCAGCATAATATTGGAGATTACAAAGA
TCATCCAAAATATATTGCAAAGAGTATAAATAAGATTGACCAGATGAGCCACTTATTGGAAGAAGTACTGGAGTCTTCTA
AATTCCAAGAGTGGACAGAGTGTCGTGAGACCTTGACTGTTAAGCCAGTTTTAGTAGATATTTTATCACGTTATCAAGAA
TTAGCTCATTCAATAGGTGTTACAATTGAAAATCAATTGACAGATGCTACCAGGGTCGTCATGAGTCTTAGGGCATTGGA
TAAGGTTTTGACAAACCTGATTAGTAATGCAATTAAATATTCAGATAAAAATGGGCGCGTAATCATATCCGAGCAAGATG
GCTATCTTTCTATCAAGAATACATGTGCGCCTCTAAGTGACCAAGAACTAGAACATTTATTTGATATATTCTATCATTCT
CAAATCGTGACAGATAAGGATGAAAGTTCCGGTTTGGGTCTTTACATTGTGAATAATATTTTAGAAAGCTATCAAATGGA
TTATAGTTTTCTCCCTTATGAACACGGTATGGAATTTAAGATTAGCTTGTAG

Upstream 100 bases:

>100_bases
CTTGTGATAGGGTCGTTGATGTCTATATTCGTACTCTTCGCAAAAAATTAGCTTTAGATTGTATCGTGACTGTGAAAAAT
GTTGGGTATAAGATTAGCTT

Downstream 100 bases:

>100_bases
ACAGATTAGTTTTTTATTAAAGTTCATATAGGGTTAACATAAGTGTGTTATTCTTTGTGTAGATAAAAGAAAGGATACTA
ATATGGTATTAGCGATTATT

Product: sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin B-type resistance protein vanSB; Vancomycin histidine protein kinase [H]

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGEKFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISA
HLKRDIVDKRLPLVHDLDIKDGKLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHELESRNEQIVKLQNQKVSFVRGA
SHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSINKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQE
LAHSIGVTIENQLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEHLFDIFYHS
QIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL

Sequences:

>Translated_443_residues
MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGEKFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISA
HLKRDIVDKRLPLVHDLDIKDGKLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHELESRNEQIVKLQNQKVSFVRGA
SHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSINKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQE
LAHSIGVTIENQLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEHLFDIFYHS
QIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL
>Mature_443_residues
MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGEKFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISA
HLKRDIVDKRLPLVHDLDIKDGKLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHELESRNEQIVKLQNQKVSFVRGA
SHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSINKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQE
LAHSIGVTIENQLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEHLFDIFYHS
QIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=267, Percent_Identity=25.8426966292135, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI87082128, Length=337, Percent_Identity=25.5192878338279, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50989; Mature: 50989

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGEKFNESARVFTEYLKTK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHHCC
TSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDGKLSNYIVMLDMSVSTADG
CCHHHHHHHHHHHHCEEEEHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEEEEECCCC
KQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFSYFYTKRLLNPLFYISEVTSK
CEEEEEEEHHHHHHHHHHCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHELESRNEQIVKLQNQKVSFVRGA
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEHHHCCHHHHHCCC
SHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSINKIDQMSHLLEEVLESSKFQEWTE
CHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
CRETLTVKPVLVDILSRYQELAHSIGVTIENQLTDATRVVMSLRALDKVLTNLISNAIKY
HHHHCCHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SDKNGRVIISEQDGYLSIKNTCAPLSDQELEHLFDIFYHSQIVTDKDESSGLGLYIVNNI
CCCCCEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
LESYQMDYSFLPYEHGMEFKISL
HHHHCCCCCCCCCCCCCCEEEEC
>Mature Secondary Structure
MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGEKFNESARVFTEYLKTK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHHCC
TSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDGKLSNYIVMLDMSVSTADG
CCHHHHHHHHHHHHCEEEEHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEEEEECCCC
KQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFSYFYTKRLLNPLFYISEVTSK
CEEEEEEEHHHHHHHHHHCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHELESRNEQIVKLQNQKVSFVRGA
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEHHHCCHHHHHCCC
SHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSINKIDQMSHLLEEVLESSKFQEWTE
CHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
CRETLTVKPVLVDILSRYQELAHSIGVTIENQLTDATRVVMSLRALDKVLTNLISNAIKY
HHHHCCHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SDKNGRVIISEQDGYLSIKNTCAPLSDQELEHLFDIFYHSQIVTDKDESSGLGLYIVNNI
CCCCCEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
LESYQMDYSFLPYEHGMEFKISL
HHHHCCCCCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8631706; 12663927 [H]