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The map label for this gene is glpK [H]

Identifier: 116516212

GI number: 116516212

Start: 1989716

End: 1991224

Strand: Reverse

Name: glpK [H]

Synonym: SPD_2013

Alternate gene names: 116516212

Gene position: 1991224-1989716 (Counterclockwise)

Preceding gene: 116516714

Following gene: 116516239

Centisome position: 97.32

GC content: 44.14

Gene sequence:

>1509_bases
ATGTCACAAGAAAAATACATCATGGCTATTGACCAGGGAACTACAAGTTCTCGTGCCATCATTTTCAACAAAAAAGGGGA
AAAGGTTAGCTCGAGTCAAAAAGAGTTTACCCAGATTTTCCCTCAGGCAGGTTGGGTTGAGCACAATGCCAATGAAATTT
GGAACTCTGTTCAGTCAGTTATTGCGGGTGCTTTCATTGAAAGTGGTGTCAAGCCAAATCAAATCGAGGCAATCGGGATT
ACCAACCAACGTGAAACAACGGTTGTCTGGGATAAGAAAACAGGACTTCCTATCTACAATGCTATCGTTTGGCAGTCACG
CCAGACAGCACCTTTGGCTGAGCAACTAAAAAGCCAAGGTTATGTGGAAAAATTCCATGAAAAGACTGGTTTGATTATTG
ATGCTTACTTCTCTGCTACCAAGGTTCGTTGGATTTTGGATCATGTAGAAGGTGCTCAAGAGCGAGCAGAAAAAGGGGAA
TTGCTCTTTGGTACTATCGATACTTGGTTGGTTTGGAAATTGACTGACGGTGCGGCTCACGTGACTGACTACTCAAATGC
AGCTCGTACCATGCTTTATAACATTAAAGAACTCAAATGGGATGATGAGATTTTGGAAATCCTTAACATTCCGAAGGCTA
TACTTCCAGAAGTTCGTTCTAACTCCGAAATCTACGGCAAGACAGCTCCATTCCATTTCTACGGTGGAGAGGTGCCAATC
TCAGGTATGGCTGGGGACCAACAAGCAGCCCTCTTTGGACAGTTGGCTTTTGAGCCAGGTATGGTTAAGAATACTTATGG
AACAGGCTCTTTCATCATCATGAATACTGGGGAAGAGATGCAGTTGTCTGAAAACAACCTCTTGACAACCATTGGTTACG
GAATCAACGGTAAGGTTTATTATGCCTTGGAAGGTTCTATCTTCATCGCAGGAAGTGCTATTCAGTGGCTTCGTGACGGT
CTTCGCATGGTTGAAAATTCACCAGAATCTGAAAAATACGCTCGTGATTCTCACAACAACGATGAAGTTTATGTCGTTCC
AGCCTTTACAGGTCTAGGCGCTCCATACTGGAACCAAAATGCTCGTGGTTCCGTCTTTGGTTTGACTCGTGGAACAAGCA
AAGAAGACTTTATCAAGGCGACTTTGCAATCTATTGCTTATCAAGTGCGTGACATCATCGACACTATGCAAGTGGATACT
CAGACTGCCATTCAAGTACTGAAGGTGGATGGTGGTGCAGCCATGAACAACTTCCTCATGCAGTTCCAGGCGGATATTTT
AGGCATTGACATTGCACGTGCTAAAAACTTGGAAACAACAGCTCTAGGAGCGGCCTTCCTAGCAGGTTTATCAGTAGGAT
ACTGGAAAGACTTGGACGAGTTGAAACTCTTGAACGAGACAGGAGAACTCTTTGAGCCATCTATGAACGAATCTCGCAAG
GAACAACTCTACAAGGGCTGGAAGAAGGCTGTGAAAGCAACTCAAGTCTTTGCGGAAGTAGACGACTAA

Upstream 100 bases:

>100_bases
TAAAAATTAAAAAAAGTTGATAAACAAGAAAGCGCTTTATTTTGTATACTAGTAAGTGTAAAGAGGAAACACCTCAAGAT
CTTTATCAGGAGGACAGTAC

Downstream 100 bases:

>100_bases
TACTGGCAGAATAAAGCGATTTATTTAGAAAGTGTGTAAATATGGAATTTTCAAAAAAAACACGTGAATTGTCAATTAAA
AAAATGCAGGAACGCACCCT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAGWVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGI
TNQRETTVVWDKKTGLPIYNAIVWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEVRSNSEIYGKTAPFHFYGGEVPI
SGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNTGEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDG
LRMVENSPESEKYARDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDIIDTMQVDT
QTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGLSVGYWKDLDELKLLNETGELFEPSMNESRK
EQLYKGWKKAVKATQVFAEVDD

Sequences:

>Translated_502_residues
MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAGWVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGI
TNQRETTVVWDKKTGLPIYNAIVWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEVRSNSEIYGKTAPFHFYGGEVPI
SGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNTGEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDG
LRMVENSPESEKYARDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDIIDTMQVDT
QTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGLSVGYWKDLDELKLLNETGELFEPSMNESRK
EQLYKGWKKAVKATQVFAEVDD
>Mature_501_residues
SQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAGWVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGIT
NQRETTVVWDKKTGLPIYNAIVWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGEL
LFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEVRSNSEIYGKTAPFHFYGGEVPIS
GMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNTGEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGL
RMVENSPESEKYARDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDIIDTMQVDTQ
TAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGLSVGYWKDLDELKLLNETGELFEPSMNESRKE
QLYKGWKKAVKATQVFAEVDD

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=496, Percent_Identity=46.5725806451613, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI4504007, Length=496, Percent_Identity=46.5725806451613, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI42794763, Length=500, Percent_Identity=46.2, Blast_Score=414, Evalue=1e-116,
Organism=Homo sapiens, GI41393575, Length=496, Percent_Identity=45.1612903225806, Blast_Score=404, Evalue=1e-112,
Organism=Homo sapiens, GI88196792, Length=503, Percent_Identity=32.6043737574553, Blast_Score=308, Evalue=6e-84,
Organism=Escherichia coli, GI1790361, Length=502, Percent_Identity=56.1752988047809, Blast_Score=610, Evalue=1e-176,
Organism=Escherichia coli, GI1790005, Length=518, Percent_Identity=23.1660231660232, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789987, Length=463, Percent_Identity=23.7580993520518, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787789, Length=488, Percent_Identity=23.3606557377049, Blast_Score=86, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17535599, Length=507, Percent_Identity=40.4339250493097, Blast_Score=373, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71986140, Length=516, Percent_Identity=25.1937984496124, Blast_Score=171, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI71986147, Length=294, Percent_Identity=30.6122448979592, Blast_Score=122, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6321755, Length=503, Percent_Identity=33.9960238568588, Blast_Score=300, Evalue=2e-82,
Organism=Drosophila melanogaster, GI17864214, Length=500, Percent_Identity=43, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24654573, Length=452, Percent_Identity=43.141592920354, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655555, Length=501, Percent_Identity=38.3233532934132, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24655560, Length=501, Percent_Identity=38.3233532934132, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24655570, Length=501, Percent_Identity=38.3233532934132, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24655574, Length=501, Percent_Identity=38.3233532934132, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24655565, Length=501, Percent_Identity=38.3233532934132, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI19922058, Length=522, Percent_Identity=33.7164750957854, Blast_Score=301, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24652871, Length=489, Percent_Identity=33.7423312883436, Blast_Score=281, Evalue=5e-76,
Organism=Drosophila melanogaster, GI24656367, Length=492, Percent_Identity=27.6422764227642, Blast_Score=221, Evalue=8e-58,
Organism=Drosophila melanogaster, GI21357867, Length=508, Percent_Identity=26.3779527559055, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24656372, Length=328, Percent_Identity=28.9634146341463, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI45552909, Length=280, Percent_Identity=25.7142857142857, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 55851; Mature: 55720

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAGWVEHNANEIWNSVQSV
CCCCCEEEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
IAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAIVWQSRQTAPLAEQLKSQG
HHHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCCHHHHEEECCCCCCHHHHHHHHCC
YVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGELLFGTIDTWLVWKLTDGAAH
HHHHHHHHCCEEEEECCCHHHHHHHHHHHHCHHHHHHCCCEEEEEECEEEEEEEECCCEE
VTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEVRSNSEIYGKTAPFHFYGGEVPI
ECCCHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHCCCCCEEECCCCEEEECCCEEE
SGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNTGEEMQLSENNLLTTIGYGINGKVY
CCCCCCCHHHHHHHHHCCCCCEEECCCCCEEEEEECCCCEEECCCCEEEEEECCCCCEEE
YALEGSIFIAGSAIQWLRDGLRMVENSPESEKYARDSHNNDEVYVVPAFTGLGAPYWNQN
EEEECCEEEECHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCCCCC
ARGSVFGLTRGTSKEDFIKATLQSIAYQVRDIIDTMQVDTQTAIQVLKVDGGAAMNNFLM
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEEECCCHHHHHHHH
QFQADILGIDIARAKNLETTALGAAFLAGLSVGYWKDLDELKLLNETGELFEPSMNESRK
HHHHHHHEEEHHHCCCCCHHHHHHHHHHHCCCHHHCCHHHHHHHHHCHHHCCCCCCHHHH
EQLYKGWKKAVKATQVFAEVDD
HHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAGWVEHNANEIWNSVQSV
CCCCEEEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
IAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAIVWQSRQTAPLAEQLKSQG
HHHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCCHHHHEEECCCCCCHHHHHHHHCC
YVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGELLFGTIDTWLVWKLTDGAAH
HHHHHHHHCCEEEEECCCHHHHHHHHHHHHCHHHHHHCCCEEEEEECEEEEEEEECCCEE
VTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEVRSNSEIYGKTAPFHFYGGEVPI
ECCCHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHCCCCCEEECCCCEEEECCCEEE
SGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNTGEEMQLSENNLLTTIGYGINGKVY
CCCCCCCHHHHHHHHHCCCCCEEECCCCCEEEEEECCCCEEECCCCEEEEEECCCCCEEE
YALEGSIFIAGSAIQWLRDGLRMVENSPESEKYARDSHNNDEVYVVPAFTGLGAPYWNQN
EEEECCEEEECHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCCCCC
ARGSVFGLTRGTSKEDFIKATLQSIAYQVRDIIDTMQVDTQTAIQVLKVDGGAAMNNFLM
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEEECCCHHHHHHHH
QFQADILGIDIARAKNLETTALGAAFLAGLSVGYWKDLDELKLLNETGELFEPSMNESRK
HHHHHHHEEEHHHCCCCCHHHHHHHHHHHCCCHHHCCHHHHHHHHHCHHHCCCCCCHHHH
EQLYKGWKKAVKATQVFAEVDD
HHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA