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The map label for this gene is glnA [H]

Identifier: 116516090

GI number: 116516090

Start: 456041

End: 457387

Strand: Direct

Name: glnA [H]

Synonym: SPD_0448

Alternate gene names: 116516090

Gene position: 456041-457387 (Clockwise)

Preceding gene: 116517159

Following gene: 116516122

Centisome position: 22.29

GC content: 42.46

Gene sequence:

>1347_bases
ATGCCAATCACAGCTGCAGATATTCGTCGTGAAGTCAAGGAAAAAAATGTTACCTTTATTCGTCTTATGTTCTCAGATAT
TTTGGGAACCATGAAAAACGTCGAAATTCCTGCTACAGATGAACAGTTAGATAAGGTCTTGTCGAACAAGGTTATGTTTG
ATGGATCTTCTATTGAAGGTTTTGTACGTATCAATGAGTCGGATATGTACTTGTACCCGGACTTGGATACATGGACAGTC
TTCCCTTGGGGAGATGAAAATGGAAGTGTTGCAGGTCTGATCTGTGATGTCTATACAACAGAAGGTGAACCATTTGCGGG
TGACCCTCGTGGTAATTTGAAACGAGCTCTTCGTCACATGGAAGAAGTTGGATTCAAATCCTTCAACCTTGGTCCAGAGC
CAGAATTCTTCCTATTTAAGTTGGATGAAAATGGGGACCCAACACTTGAAGTGAATGACAAGGGTGGCTACTTTGATTTG
GCACCTACTGACCTTGCGGACAACACACGTCGTGAGATTGTGAATGTCTTGACCAAAATGGGATTTGAAGTAGAAGCGAG
TCACCACGAGGTTGCGGTTGGACAGCATGAGATTGACTTTAAGTACGATGAAGTTCTCCGTGCTTGTGATAAGATTCAAA
TCTTTAAGCTTGTTGTTAAAACCATTGCTCGCAAACACGGACTTTACGCAACATTTATGGCGAAGCCAAAATTTGGTATT
GCTGGATCAGGTATGCACTGTAATATGTCCTTGTTTGATGCAGAAGGAAATAACGCCTTCTTTGATCCAAATGATCCAAA
AGGAATGCAGTTGTCAGAAACAGCTTACCATTTCCTAGGCGGTTTGATCAAGCATGCTTACAACTATACTGCCATCATGA
ACCCAACAGTTAACTCATACAAACGTTTGGTTCCAGGTTATGAAGCGCCTGTTTACATTGCTTGGGCTGGTCGTAACCGT
TCGCCACTTGTGCGCGTACCTGCTTCACGTGGTATGGGAACTCGTCTTGAGTTGCGTTCAGTGGATCCAATGGCGAACCC
TTACGTTGCTATGGCTGTTCTTTTGGAAGTTGGTTTGTATGGTATTGAAAATAAAATCGAAGCACCAGCTCCTATCGAAG
AAAATATCTACATCATGACAGCAGAAGAGCGCAAGGAAGCTGGTATTACAGACCTTCCATCAACTCTTCACAACGCTTTG
AAAGCTTTGACAGAAGATGAAGTGGTTAAAGCTGCTCTCGGAGATCACATCTACACTAGCTTCCTTGAAGCCAAACGAAT
CGAATGGGCAAGTTATGCAACCTTCGTTTCACAATGGGAAATTGATAATTATTTAGACCTTTACTAA

Upstream 100 bases:

>100_bases
TCACAATGAACTCCTCCAACAGGGGCGTTTTGCTTCAGTACAGTCACCTTTTGGTCGCGGTTAGGCAACCGCAAGTAGTC
ATAGATTAAGGAGAAAACTT

Downstream 100 bases:

>100_bases
TACTAATATAGAAGAAAGATTGCCTGTGGGTAAACAGTCACACAGCAGTCTAAGCCATCAAATACCTATAACCCTTAAAA
TAGCGATTTCGGTCGCTATT

Product: glutamine synthetase, type I

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLDKVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTV
FPWGDENGSVAGLICDVYTTEGEPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLVVKTIARKHGLYATFMAKPKFGI
AGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAYHFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNR
SPLVRVPASRGMGTRLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDLPSTLHNAL
KALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY

Sequences:

>Translated_448_residues
MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLDKVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTV
FPWGDENGSVAGLICDVYTTEGEPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLVVKTIARKHGLYATFMAKPKFGI
AGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAYHFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNR
SPLVRVPASRGMGTRLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDLPSTLHNAL
KALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY
>Mature_447_residues
PITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLDKVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVF
PWGDENGSVAGLICDVYTTEGEPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDLA
PTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLVVKTIARKHGLYATFMAKPKFGIA
GSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAYHFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRS
PLVRVPASRGMGTRLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDLPSTLHNALK
ALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=443, Percent_Identity=27.313769751693, Blast_Score=136, Evalue=4e-32,
Organism=Escherichia coli, GI1790301, Length=461, Percent_Identity=39.6963123644252, Blast_Score=272, Evalue=3e-74,
Organism=Escherichia coli, GI87081870, Length=459, Percent_Identity=25.7080610021786, Blast_Score=132, Evalue=3e-32,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 50323; Mature: 50192

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00180 GLNA_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLDKVLSNKVMFDGSSIEG
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCEEECCCCCEE
FVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEGEPFAGDPRGNLKRALRHM
EEEECCCCEEEECCCCCEEEEECCCCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHH
EEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDLAPTDLADNTRREIVNVLTKM
HHCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHC
GFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLVVKTIARKHGLYATFMAKPKFGI
CCEEECCCCEEEECCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
AGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAYHFLGGLIKHAYNYTAIMNPTVNSY
CCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHH
KRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGTRLELRSVDPMANPYVAMAVLLEVGLY
HHHCCCCCCCEEEEECCCCCCCEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
GIENKIEAPAPIEENIYIMTAEERKEAGITDLPSTLHNALKALTEDEVVKAALGDHIYTS
CCCCCCCCCCCCCCCEEEEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLEAKRIEWASYATFVSQWEIDNYLDLY
HHHHHHCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLDKVLSNKVMFDGSSIEG
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCEEECCCCCEE
FVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEGEPFAGDPRGNLKRALRHM
EEEECCCCEEEECCCCCEEEEECCCCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHH
EEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDLAPTDLADNTRREIVNVLTKM
HHCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHC
GFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLVVKTIARKHGLYATFMAKPKFGI
CCEEECCCCEEEECCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
AGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAYHFLGGLIKHAYNYTAIMNPTVNSY
CCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHH
KRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGTRLELRSVDPMANPYVAMAVLLEVGLY
HHHCCCCCCCEEEEECCCCCCCEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
GIENKIEAPAPIEENIYIMTAEERKEAGITDLPSTLHNALKALTEDEVVKAALGDHIYTS
CCCCCCCCCCCCCCCEEEEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLEAKRIEWASYATFVSQWEIDNYLDLY
HHHHHHCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]