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The map label for this gene is epsM [H]

Identifier: 116516041

GI number: 116516041

Start: 959932

End: 960531

Strand: Direct

Name: epsM [H]

Synonym: SPD_0949

Alternate gene names: 116516041

Gene position: 959932-960531 (Clockwise)

Preceding gene: 116516989

Following gene: 116515531

Centisome position: 46.91

GC content: 32.67

Gene sequence:

>600_bases
TTGGCGGTATTGTCAATTTATGAAGGTGAAGTTGAGGGATTGGTAGATATTTTTGAGGAAGGTCGCTTTATTGGTTGCAA
AATCTTAGATAAAACTGTTTTAGGGGGATTAAAATGGTTTGAAAGTTTACTCTTAACACTTGATAGGAAGCCTAGAGCAA
TTGTTGCATTTGGTGATAATTTTAAGCGCGAAGAAATATTTTGTTCATATAAAAATAAGGTGGAGTACATTAATGTTATA
TGTAATTCCGCGAGAATTTTTAAACATTCTTTTTTAGGAAAAGGAAATTTTATTGGTACCAATGTGACTATTCAAGCACT
TGTAGAAATCGGTGACAACAATATTATAAATTCTGGTAGTATTGTTTCATGTAATTGTAAAATTGGTAATAATGTAAATA
TTTCCCCAGGTGTTATATTATCTGGAAATGTTAAAATTGATGATAACGTTTTTATTGGTGCAGGAGCAACAATTAGAGAT
GCGGTAAGCATTGGCTTTGGTGCTATAATTGGTGCAGGTGCTACCGTAATACATAACGTACCGGAAAATGCTGTGGTAGT
AGGAACACCAGGGAAAATAATAAAATATAGATCAGTTTGA

Upstream 100 bases:

>100_bases
GTGGCAAGATAAAAATACAATATATAAAGAGAAATAATCATAAGTTTGTTTTAAAATGTGGGTAAAGGATATGTTTATAC
TAGGTTCAGGAGATCAAGGA

Downstream 100 bases:

>100_bases
AGAGAAACTCTTCATTGCCATATAACTTAAGTTTCATCGCATGTCAGTAATCTCATAAGGAATTAGTTAGATATTAGTGT
TAGCTCCTAGGTTTCAACTG

Product: hexapeptide repeat-containing transferase

Products: CoA; acetylmaltose [C]

Alternate protein names: NA

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MAVLSIYEGEVEGLVDIFEEGRFIGCKILDKTVLGGLKWFESLLLTLDRKPRAIVAFGDNFKREEIFCSYKNKVEYINVI
CNSARIFKHSFLGKGNFIGTNVTIQALVEIGDNNIINSGSIVSCNCKIGNNVNISPGVILSGNVKIDDNVFIGAGATIRD
AVSIGFGAIIGAGATVIHNVPENAVVVGTPGKIIKYRSV

Sequences:

>Translated_199_residues
MAVLSIYEGEVEGLVDIFEEGRFIGCKILDKTVLGGLKWFESLLLTLDRKPRAIVAFGDNFKREEIFCSYKNKVEYINVI
CNSARIFKHSFLGKGNFIGTNVTIQALVEIGDNNIINSGSIVSCNCKIGNNVNISPGVILSGNVKIDDNVFIGAGATIRD
AVSIGFGAIIGAGATVIHNVPENAVVVGTPGKIIKYRSV
>Mature_198_residues
AVLSIYEGEVEGLVDIFEEGRFIGCKILDKTVLGGLKWFESLLLTLDRKPRAIVAFGDNFKREEIFCSYKNKVEYINVIC
NSARIFKHSFLGKGNFIGTNVTIQALVEIGDNNIINSGSIVSCNCKIGNNVNISPGVILSGNVKIDDNVFIGAGATIRDA
VSIGFGAIIGAGATVIHNVPENAVVVGTPGKIIKYRSV

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR013817
- InterPro:   IPR020019
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.79 [C]

Molecular weight: Translated: 21367; Mature: 21236

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS00101 HEXAPEP_TRANSFERASES ; PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVLSIYEGEVEGLVDIFEEGRFIGCKILDKTVLGGLKWFESLLLTLDRKPRAIVAFGDN
CEEEEEECCCHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
FKREEIFCSYKNKVEYINVICNSARIFKHSFLGKGNFIGTNVTIQALVEIGDNNIINSGS
CCCCCEEEECCCCEEEEEEEECCHHEEHHHHCCCCCEEECCEEEEEEEEECCCCEECCCC
IVSCNCKIGNNVNISPGVILSGNVKIDDNVFIGAGATIRDAVSIGFGAIIGAGATVIHNV
EEEEEEEECCCCCCCCCEEEECCEEECCCEEEECCCCHHHHHHHCHHHHHCCCCEEEECC
PENAVVVGTPGKIIKYRSV
CCCEEEEECCCCEEEEECC
>Mature Secondary Structure 
AVLSIYEGEVEGLVDIFEEGRFIGCKILDKTVLGGLKWFESLLLTLDRKPRAIVAFGDN
EEEEEECCCHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
FKREEIFCSYKNKVEYINVICNSARIFKHSFLGKGNFIGTNVTIQALVEIGDNNIINSGS
CCCCCEEEECCCCEEEEEEEECCHHEEHHHHCCCCCEEECCEEEEEEEEECCCCEECCCC
IVSCNCKIGNNVNISPGVILSGNVKIDDNVFIGAGATIRDAVSIGFGAIIGAGATVIHNV
EEEEEEEECCCCCCCCCEEEECCEEECCCEEEECCCCHHHHHHHCHHHHHCCCCEEEECC
PENAVVVGTPGKIIKYRSV
CCCEEEEECCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 0.20 {glucose} 0.11 {maltose}

Km value (mM): 90 {maltose}} 62 {glucose}} 0.018 {acetyl-CoA}} 40 {triethanolamine}} [C]

Substrates: Acetyl-CoA; maltose [C]

Specific reaction: Acetyl-CoA + maltose = CoA + acetylmaltose [C]

General reaction: Acyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]