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The map label for this gene is hsdM [H]

Identifier: 116515872

GI number: 116515872

Start: 794286

End: 795779

Strand: Direct

Name: hsdM [H]

Synonym: SPD_0782

Alternate gene names: 116515872

Gene position: 794286-795779 (Clockwise)

Preceding gene: 116517060

Following gene: 116515342

Centisome position: 38.82

GC content: 36.28

Gene sequence:

>1494_bases
ATGATTACAGGCGAATTAAAAAATAAAATCGATCAGCTGTGGGAAATTCTTTGGACAGAAGGAAACGCAAATCCTTTAAC
AAATATTGAACAGTTGACTTATCTCTTATTTATGAAAGATTTGGATAGTGTCGAGCTTGGACGTGAAAGTGATGCTGAAT
TTCTAGGGATTCCTTATGAGGGAGTTTTTCCAAAAGATAAACCTGAATACCGTTGGTCAACTTTTAAAAATATAGGAGAT
GCTCAGGAAGTTTATCGTTTAATGACTCAGGAGATTTTTCCGTTTATTAAAAATCTCAAGGGGGATACAGATGATACAGC
CTTTTCACGATATATGCGAGAAGCTATTTTTCAAATAAATAAACCTGCTACGCTTCAAAAGGCAATTTCTATCTTAGATG
TTTTTCCAACTAGGGGATTAGATGTAGATTTTGATAATGACAAACAAAGTATTACTGATATCGGAGATATCTATGAATAT
CTGTTATCAAAATTGTCGACCGCAGGTAAAAATGGACAGTTCCGTACACCTCGTCACATCATCGATATGATGGTTGAGTT
GATGCAACCGACTATCAAAGATATCATCTCAGATCCCGCTATGGGTTCTGCTGGCTTCTTAGTATCTGCTAGCCGTTACT
TAAAGCGTAAGAAAGATGAATGGGAAACCAATACAGATAATATCAATCATTTTCATAATCAGATGTTTCATGGAAATGAT
ACGGATACGACTATGTTGAGACTTGGGGCGATGAACATGATGCTACATGGAGTAGAAAATCCACAAATCAGTTACCTTGA
CTCGCTGTCTCAAGATAATGAAGAAGCCGATAAATATACTTTGGTTTTAGCAAATCCTCCTTTTAAGGGCTCACTTGACT
ACAATTCAACCTCTAATGACCTTCTTGCAACCGTAAAAACCAAAAAAACAGAATTACTCTTTCTTTCTCTTTTCTTGCGA
ACTTTAAAACCAGGTGGACGAGCAGCAGTTATCGTACCTGATGGTGTCCTTTTTGGTTCGTCTAAAGCTCATAAAGGAAT
TCGTCAGGAAATTGTAGAGAATCATAAGCTTGATGCTGTAATCTCAATGCCTAGTGGTGTGTTCAAGCCTTATGCTGGAG
TTTCAACTGCCATTCTCATCTTTACAAAAACTGGTAATGGTGGTACTGACAAAGTCTGGTTTTACGATATGAAAGCGGAT
GGTTTAAGTTTGGATGATAAGCGACAACCGATTAGCGACAATGATATTCCAGATATTATCGAACGCTTTCATCATCTTGA
AAAAGAAGCAGAACGTCAGAGAACGGATCAATCTTTCTTTGTTCCAGTTGCTGAGATAAAGGAAAATGATTATGATTTGT
CTATCAATAAATATAAAGAGATTGAGTATGAAAAAGTTGAGTATGAACCAACAGAGGTCATCTTAAAGAAAATCAATGAT
TTAGAAAAAGAAATTCAAGCTGGCTTGGCTGAATTAGAAAAATTACTCAAGTAG

Upstream 100 bases:

>100_bases
ATATTCAGGTGACGCATAGATATAGTTAATTGAAGCTTTGTTTGAAATCTGATAAAATAATGATATTACTAAGTTTTAAA
AACTAAAGAAAAGGGAAGAT

Downstream 100 bases:

>100_bases
AGAGGTGGCTGTATGAAAAAAGTGAAGTTGGGGGAAGTCTTATCTCTAAAAAAAGGCAAGAAAGCCACTGTACTTGCTGA
ACAAACAACTCTAAGCCAAC

Product: type I restriction-modification system, M subunit

Products: NA

Alternate protein names: M.EcoEI [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSVELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGD
AQEVYRLMTQEIFPFIKNLKGDTDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKKDEWETNTDNINHFHNQMFHGND
TDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEADKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLR
TLKPGGRAAVIVPDGVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKVWFYDMKAD
GLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKENDYDLSINKYKEIEYEKVEYEPTEVILKKIND
LEKEIQAGLAELEKLLK

Sequences:

>Translated_497_residues
MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSVELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGD
AQEVYRLMTQEIFPFIKNLKGDTDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKKDEWETNTDNINHFHNQMFHGND
TDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEADKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLR
TLKPGGRAAVIVPDGVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKVWFYDMKAD
GLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKENDYDLSINKYKEIEYEKVEYEPTEVILKKIND
LEKEIQAGLAELEKLLK
>Mature_497_residues
MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSVELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGD
AQEVYRLMTQEIFPFIKNLKGDTDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKKDEWETNTDNINHFHNQMFHGND
TDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEADKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLR
TLKPGGRAAVIVPDGVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKVWFYDMKAD
GLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKENDYDLSINKYKEIEYEKVEYEPTEVILKKIND
LEKEIQAGLAELEKLLK

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=486, Percent_Identity=29.4238683127572, Blast_Score=161, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 56663; Mature: 56663

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSVELGRESDAEFLGIPYE
CCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHCCCCCCCCEEECCCC
GVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGDTDDTAFSRYMREAIFQIN
CCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
KPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEYLLSKLSTAGKNGQFRTPRHI
CCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
IDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKKDEWETNTDNINHFHNQMFHGND
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCC
TDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEADKYTLVLANPPFKGSLDYNSTSND
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
LLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPDGVLFGSSKAHKGIRQEIVENHKLDAV
EEEEEECCHHHHHHHHHHHHHCCCCCCEEEEECCCEEECCCHHHHHHHHHHHHCCCCCEE
ISMPSGVFKPYAGVSTAILIFTKTGNGGTDKVWFYDMKADGLSLDDKRQPISDNDIPDII
EECCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHH
ERFHHLEKEAERQRTDQSFFVPVAEIKENDYDLSINKYKEIEYEKVEYEPTEVILKKIND
HHHHHHHHHHHHHHCCCHHEEEHHHHCCCCCCEECHHHHCCCHHHCCCCHHHHHHHHHHH
LEKEIQAGLAELEKLLK
HHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSVELGRESDAEFLGIPYE
CCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHCCCCCCCCEEECCCC
GVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGDTDDTAFSRYMREAIFQIN
CCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
KPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEYLLSKLSTAGKNGQFRTPRHI
CCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
IDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKKDEWETNTDNINHFHNQMFHGND
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCC
TDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEADKYTLVLANPPFKGSLDYNSTSND
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
LLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPDGVLFGSSKAHKGIRQEIVENHKLDAV
EEEEEECCHHHHHHHHHHHHHCCCCCCEEEEECCCEEECCCHHHHHHHHHHHHCCCCCEE
ISMPSGVFKPYAGVSTAILIFTKTGNGGTDKVWFYDMKADGLSLDDKRQPISDNDIPDII
EECCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHH
ERFHHLEKEAERQRTDQSFFVPVAEIKENDYDLSINKYKEIEYEKVEYEPTEVILKKIND
HHHHHHHHHHHHHHCCCHHEEEHHHHCCCCCCEECHHHHCCCHHHCCCCHHHHHHHHHHH
LEKEIQAGLAELEKLLK
HHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]