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The map label for this gene is pepV [H]

Identifier: 116515781

GI number: 116515781

Start: 555559

End: 556959

Strand: Direct

Name: pepV [H]

Synonym: SPD_0542

Alternate gene names: 116515781

Gene position: 555559-556959 (Clockwise)

Preceding gene: 116516933

Following gene: 116517025

Centisome position: 27.15

GC content: 44.25

Gene sequence:

>1401_bases
ATGATAGCAATTGATTTTACAGCAGAAGTAGAAAAACGCAAAGAAGACCTCTTGGCTGACTTGTTTAGTCTTTTGGAAAT
CAACTCAGAACGTGATGATAGCAAGGCTGATGCCCAACATCCATTTGGACCTGGTCCAGTAAAAGCCTTGGAAAAATTCC
TTGAAATCGCAGACCGTGATGGTTATCCAACTAAAAATGTTGATAACTATGCAGGACATTTTGAGTTTGGTGATGGAGAA
GAAGTTCTCGGAATCTTTGCCCATATGGACGTGGTTCCAGCTGGTAGCGGTTGGGATACAGATCCTTACACACCAACTAT
CAAAGATGGTCGCCTTTATGCGCGCGGGGCTTCGGACGATAAGGGTCCTACAACAGCTTGTTACTATGGTTTGAAAATCA
TCAAAGAATTGGGTCTTCCAACTTCTAAGAAAGTTCGCTTCATCGTTGGAACAGACGAAGAATCAGGCTGGGCAGACATG
GACTACTACTTTGAGCACGTAGGACTTGCCAAACCAGATTTCGGTTTCTCACCAGATGCTGAATTTCCAATCATCAATGG
TGAAAAAGGAAATATCACGGAATACCTCCACTTTGCAGGAGAAAATACAGGTGCTGCCCGTCTTCACAGCTTTACAGGTG
GTTTACGTGAAAATATGGTACCAGAATCAGCAACAGCAGTCGTTTCAGGTGATTTGGCTGACTTGCAAGCTAAACTAGAT
GCCTTTGTTGCAGAACACAAACTTAGAGGAGAACTCCAAGAAGAAGCTGGCAAATACAAGGTGACGATCATTGGTAAATC
AGCCCACGGTGCTATGCCTGCTTCAGGTGTCAATGGCGCAACTTACCTTGCCCTCTTCCTCAGCCAGTTTGGCTTTGCTG
GTCCAGCCAAAGACTACCTTGACATCGCAGGTAAAATTCTCTTGAACGATCATGAGGGTGAAAATCTCAAGATTGCTCAT
GTGGATGAAAAGATGGGTGCTCTTTCTATGAATGCCGGCGTCTTCCGCTTCGATGAAACAAGTGCTGATAATACCATTGC
CCTTAACATCCGCTATCCAAAAGGAACAAGTCCAGAACAAATCAAGTCAATCCTAGAAAACTTGCCAGTTGCTTCTGTCA
GTCTATCAGAACATGGTCATACCCCTCACTATGTGCCAATGGAAGATCCACTTGTGCAAACCTTGTTGAATGTCTATGAA
AAACAAACAGGCCTACAAGGTCATGAACAAGTCATCGGTGGTGGAACCTTTGGTCGCTTGTTAGAGCGCGGAGTTGCCTA
TGGTGCTATGTTCCCAGACTCAATTGATACCATGCACCAAGCCAATGAATTTATTGCCTTGGACGATCTCTTCCGAGCAG
CAGCAATTTATGCCGAAGCTATTTACGAATTGATCAAATAA

Upstream 100 bases:

>100_bases
AGAACTCTTGATCACAGTGGGTTACACAGACGACAAATTGGAACCAAGCTACCGCTTGCCAGTAGATGAAATCATCGAAA
AAAGATAGGAAGAAGAAAAA

Downstream 100 bases:

>100_bases
AACGATAGAAGTCTGAGATCTTATGCTTGGACTTCTTTTTGGAGGGAAAGTAGATGTCTCAAATCGAAAGAATCAAACAG
GCTATCATGGCGGATTCGCA

Product: dipeptidase PepV

Products: succinate; LL-2,6-diaminoheptanedioate

Alternate protein names: Beta-Ala-His dipeptidase; Peptidase V [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGPGPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGE
EVLGIFAHMDVVPAGSGWDTDPYTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLRENMVPESATAVVSGDLADLQAKLD
AFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASGVNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAH
VDEKMGALSMNAGVFRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLVQTLLNVYE
KQTGLQGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDLFRAAAIYAEAIYELIK

Sequences:

>Translated_466_residues
MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGPGPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGE
EVLGIFAHMDVVPAGSGWDTDPYTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLRENMVPESATAVVSGDLADLQAKLD
AFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASGVNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAH
VDEKMGALSMNAGVFRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLVQTLLNVYE
KQTGLQGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDLFRAAAIYAEAIYELIK
>Mature_466_residues
MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGPGPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGE
EVLGIFAHMDVVPAGSGWDTDPYTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLRENMVPESATAVVSGDLADLQAKLD
AFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASGVNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAH
VDEKMGALSMNAGVFRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLVQTLLNVYE
KQTGLQGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDLFRAAAIYAEAIYELIK

Specific function: Is a relatively unspecific dipeptidase cleaving a variety of dipeptides, notably those with an N-terminal beta-Ala or D-Ala residue, e.g. carnosine (beta-Ala-His). To a lesser extent, also shows aminopeptidase activity, since it is able to catalyze the re

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family [H]

Homologues:

Organism=Caenorhabditis elegans, GI193211059, Length=88, Percent_Identity=37.5, Blast_Score=66, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17563146, Length=79, Percent_Identity=36.7088607594937, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR011291
- InterPro:   IPR010964
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF01546 Peptidase_M20 [H]

EC number: 3.5.1.18

Molecular weight: Translated: 50750; Mature: 50750

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGPGPVKALEKFLEIADRD
CEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
GYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDPYTPTIKDGRLYARGASDD
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCEEEEECCCCC
KGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADMDYYFEHVGLAKPDFGFSPDA
CCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCC
EFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLRENMVPESATAVVSGDLADLQAKLD
CCCEEECCCCCHHHHHHCCCCCCCHHHHHHHHCCHHHCCCCCCHHEEEECCHHHHHHHHH
AFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASGVNGATYLALFLSQFGFAGPAKDYL
HHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHH
DIAGKILLNDHEGENLKIAHVDEKMGALSMNAGVFRFDETSADNTIALNIRYPKGTSPEQ
HCCCEEEEECCCCCCEEEEEECCHHCCEECCCCEEEECCCCCCCEEEEEEECCCCCCHHH
IKSILENLPVASVSLSEHGHTPHYVPMEDPLVQTLLNVYEKQTGLQGHEQVIGGGTFGRL
HHHHHHHCCCEEEEHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHEECCCHHHHH
LERGVAYGAMFPDSIDTMHQANEFIALDDLFRAAAIYAEAIYELIK
HHHHHHHCCCCCCHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGPGPVKALEKFLEIADRD
CEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
GYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDPYTPTIKDGRLYARGASDD
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCEEEEECCCCC
KGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADMDYYFEHVGLAKPDFGFSPDA
CCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCC
EFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLRENMVPESATAVVSGDLADLQAKLD
CCCEEECCCCCHHHHHHCCCCCCCHHHHHHHHCCHHHCCCCCCHHEEEECCHHHHHHHHH
AFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASGVNGATYLALFLSQFGFAGPAKDYL
HHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHH
DIAGKILLNDHEGENLKIAHVDEKMGALSMNAGVFRFDETSADNTIALNIRYPKGTSPEQ
HCCCEEEEECCCCCCEEEEEECCHHCCEECCCCEEEECCCCCCCEEEEEEECCCCCCHHH
IKSILENLPVASVSLSEHGHTPHYVPMEDPLVQTLLNVYEKQTGLQGHEQVIGGGTFGRL
HHHHHHHCCCEEEEHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHEECCCHHHHH
LERGVAYGAMFPDSIDTMHQANEFIALDDLFRAAAIYAEAIYELIK
HHHHHHHCCCCCCHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-succinyl-LL-2,6-diaminoheptanedioate; H2O

Specific reaction: N-succinyl-LL-2,6-diaminoheptanedioate + H2O = succinate + LL-2,6-diaminoheptanedioate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7528082 [H]