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The map label for this gene is fmt

Identifier: 116515769

GI number: 116515769

Start: 1567672

End: 1568607

Strand: Reverse

Name: fmt

Synonym: SPD_1545

Alternate gene names: 116515769

Gene position: 1568607-1567672 (Counterclockwise)

Preceding gene: 116516961

Following gene: 116517030

Centisome position: 76.66

GC content: 45.94

Gene sequence:

>936_bases
ATGACAAAACTAATCTTTATGGGGACCCCCGACTTTTCAGCAACAGTCTTAAAAGGACTTTTGACAGATGACCGTTACGA
AATTCTAGCCGTTGTGACCCAGCCAGACCGTGCTGTTGGTCGTAAAAAAGTTATCCAAGAAACCCCAGTCAAGCAGGCTG
CCAAGGAAGCAGGATTATCTATCTACCAACCTGAAAAATTATCTGGAAGTCCAGAGATGGAAGATCTTATGAAGCTAGGA
GCAGATGGAATTGTGACTGCTGCTTTTGGGCAGTTTCTCCCAAGCAAACTCCTTGATAGCATGGACTTTGCTGTCAACGT
TCATGCCTCCCTCCTTCCTAGACACCGTGGTGGTGCGCCTATCCATTATGCCTTGATTCAAGGGGATGAGGAAGCTGGTG
TGACCATCATGGAAATGGTTAAGGAAATGGATGCAGGAGATATGATTTCTCGTCGCAGCATTCCGATCACAGATGAGGAC
AATGTTGGCACCTTGTTTGAAAAATTGGCGCTAGTTGGTCGTGATTTGCTTTTGGACACTCTGCCTGCCTATATTGCTGG
TGATATCAAACCTGAACCGCAGGATACGAGTCAGGTTACCTTCTCTCCAAATATAAAGTCAGAGGAAGAAAAACTGAACT
GGAACAAAACCAATCGTCAACTCTTTAACCAAATTCGTGGAATGAACCCCTGGCCTGTTGCCCATACTTTCCTTAAGGGC
GACCGCTTTAAGATTTATGAAGCCCTACCAGTAGAAGGTCAGGGAAATCCAGGTGAGATTCTCTCTATCGGCAAGAAAGA
ATTGATTGTCGCAACGGCTGAAGGGGCTCTATCCCTCAAACAAGTGCAGCCAGCTGGTAAGCCTAAGATGGACATTGCTT
CCTTCCTCAACGGAGTTGGACGTACATTGACTGTAGGAGAACGATTTGGTGACTAA

Upstream 100 bases:

>100_bases
CCTCAACCAGGTCCTGGCCTTGACTCAAGAACGGGAAAATAGTGAGCTCCGTCTCAGCATTGACCATGAGCCGCAGCAAT
TTTTATAAGAAGGAGAAGAT

Downstream 100 bases:

>100_bases
AGTAGAAACGGCTAGAAGTTTAGCTCTAGCAGTGCTAGAGGATGTTTTTGTGAACCAAGCATATTCAAATATCGCCTTAA
ATAAACACCTCAAGGGGAGT

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKVIQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLG
ADGIVTAAFGQFLPSKLLDSMDFAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKSEEEKLNWNKTNRQLFNQIRGMNPWPVAHTFLKG
DRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGALSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD

Sequences:

>Translated_311_residues
MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKVIQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLG
ADGIVTAAFGQFLPSKLLDSMDFAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKSEEEKLNWNKTNRQLFNQIRGMNPWPVAHTFLKG
DRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGALSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD
>Mature_310_residues
TKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKVIQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGA
DGIVTAAFGQFLPSKLLDSMDFAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDEDN
VGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKSEEEKLNWNKTNRQLFNQIRGMNPWPVAHTFLKGD
RFKIYEALPVEGQGNPGEILSIGKKELIVATAEGALSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI238814322, Length=268, Percent_Identity=26.1194029850746, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI21614513, Length=303, Percent_Identity=22.7722772277228, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI164663775, Length=235, Percent_Identity=26.8085106382979, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1789683, Length=310, Percent_Identity=40.6451612903226, Blast_Score=224, Evalue=4e-60,
Organism=Escherichia coli, GI1788589, Length=281, Percent_Identity=29.8932384341637, Blast_Score=127, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI133930964, Length=259, Percent_Identity=28.5714285714286, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6319458, Length=289, Percent_Identity=24.9134948096886, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45550868, Length=321, Percent_Identity=27.4143302180685, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI28571984, Length=237, Percent_Identity=29.535864978903, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24585660, Length=247, Percent_Identity=25.1012145748988, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_STRP2 (Q04J40)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816998.1
- ProteinModelPortal:   Q04J40
- SMR:   Q04J40
- STRING:   Q04J40
- EnsemblBacteria:   EBSTRT00000020660
- GeneID:   4441669
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1545
- eggNOG:   COG0223
- GeneTree:   EBGT00050000028330
- HOGENOM:   HBG571560
- OMA:   VVAYGAI
- ProtClustDB:   PRK00005
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 33977; Mature: 33845

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKVIQETPVKQAAKEAGLS
CCEEEEEECCCHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHHCCHHHHHHHCCCE
IYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMDFAVNVHASLLPRHRGGAP
EECCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEHHHHCCCCCCCCC
IHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDEDNVGTLFEKLALVGRDLLLDT
EEEEEEECCCCCCCHHHHHHHHCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LPAYIAGDIKPEPQDTSQVTFSPNIKSEEEKLNWNKTNRQLFNQIRGMNPWPVAHTFLKG
HHHHHCCCCCCCCCCCCEEEECCCCCCHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHCC
DRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGALSLKQVQPAGKPKMDIASFLNGVG
CCEEEEEEECCCCCCCCCHHEECCCCEEEEEECCCCEEHHHCCCCCCCCHHHHHHHHHCC
RTLTVGERFGD
CEEECCHHCCC
>Mature Secondary Structure 
TKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKVIQETPVKQAAKEAGLS
CEEEEEECCCHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHHCCHHHHHHHCCCE
IYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMDFAVNVHASLLPRHRGGAP
EECCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEHHHHCCCCCCCCC
IHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDEDNVGTLFEKLALVGRDLLLDT
EEEEEEECCCCCCCHHHHHHHHCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LPAYIAGDIKPEPQDTSQVTFSPNIKSEEEKLNWNKTNRQLFNQIRGMNPWPVAHTFLKG
HHHHHCCCCCCCCCCCCEEEECCCCCCHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHCC
DRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGALSLKQVQPAGKPKMDIASFLNGVG
CCEEEEEEECCCCCCCCCHHEECCCCEEEEEECCCCEEHHHCCCCCCCCHHHHHHHHHCC
RTLTVGERFGD
CEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA