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The map label for this gene is regR [H]

Identifier: 116515692

GI number: 116515692

Start: 298962

End: 299963

Strand: Direct

Name: regR [H]

Synonym: SPD_0301

Alternate gene names: 116515692

Gene position: 298962-299963 (Clockwise)

Preceding gene: 116516834

Following gene: 116515442

Centisome position: 14.61

GC content: 39.82

Gene sequence:

>1002_bases
TTGGAGAAGAAACTGACCATAAAAGACATTGCGGAAATGGCTCAGACCTCGAAAACAACCGTGTCATTTTACCTAAACGG
GAAATATGAAAAAATGTCCCAAGAGACACGTGAAAAGATTGAAAAAGTTATTCATGAAACAAATTACAAACCGAGCATTG
TTGCGCGTAGCTTAAACTCCAAACGAACAAAATTAATCGGTGTTTTGATCGGTGATATTACCAACAGTTTCTCAAACCAA
ATTGTTAAGGGAATTGAGGATATCGCCAGCCAGAATGGCTACCAGGTAATGATAGGAAATAGTAATTACAGCCAAGAGAG
TGAGGACCGGTATATTGAAAGCATGCTTCTCTTGGGAGTAGACGGCTTTATTATTCAGCCGACCTCTAATTTCCGAAAAT
ATTCTCGTATCATCGATGAGAAAAAGAAGAAAATGGTCTTTTTTGATAGTCAGCTCTATGAACACCGGACTAGCTGGGTT
AAAACCAATAACTATGATGCCGTTTATGACATGACCCAGTCCTGTATCGAAAAAGGTTATGAGTATTTTCTCTTGATTAC
AGCGGATACGAGTCGTTTGAGTACTCGGATTGAGCGGGCAAGTGGTTTTGTGGATGCTTTAACAGATGCTAATATGCGTC
ACGCCAGTCTAACCATTGAAGATAAGCATACGAATTTGGAACAAATTAAGGAATTTTTACAAAAAGAAATCGATCCCGAT
GAAAAAACTCTGGTATTTATCCCTAACTGTTGGGCCCTACCTCTAGTCTTTACCGTTATCAAAGAGTTGAATTATAACTT
GCCGCAAGTTGGGTTGATTGGTTTTGACAATACGGAGTGGACTTGCTTTTCTTCTCCAAGTGTTTCGACGCTGGTTCAGC
CCTCCTTTGAGGAAGGACAACAGGCTACAAAGATTTTGATTGACCAGATTGAAGGCCGCAATCAAGAAGAAAGGCAACAA
GTCTTGGATTGTAGTGTGAATTGGAAAGAGTCGACTTTCTAA

Upstream 100 bases:

>100_bases
ATGATAAAATAAATGAGAGAATGATTCGCTTGCAGTGCTAGAAATAGGCATTTTGAATAGTGAATATGTTATAATAAGTA
TTAGTAGGAGGTGTTTTAGA

Downstream 100 bases:

>100_bases
AATGAAGGAAAATGACTTGCAATCTCTGTTAAGAAATAAAATAATCCCACCTAGAACAAGCTAGGTGGGATTATTTGCCT
ATGAAATGAGAAATTATGGG

Product: sugar binding transcriptional regulator RegR

Products: NA

Alternate protein names: Kdg operon repressor [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVIHETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQ
IVKGIEDIASQNGYQVMIGNSNYSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
KTNNYDAVYDMTQSCIEKGYEYFLLITADTSRLSTRIERASGFVDALTDANMRHASLTIEDKHTNLEQIKEFLQKEIDPD
EKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFDNTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQ
VLDCSVNWKESTF

Sequences:

>Translated_333_residues
MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVIHETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQ
IVKGIEDIASQNGYQVMIGNSNYSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
KTNNYDAVYDMTQSCIEKGYEYFLLITADTSRLSTRIERASGFVDALTDANMRHASLTIEDKHTNLEQIKEFLQKEIDPD
EKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFDNTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQ
VLDCSVNWKESTF
>Mature_333_residues
MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVIHETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQ
IVKGIEDIASQNGYQVMIGNSNYSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
KTNNYDAVYDMTQSCIEKGYEYFLLITADTSRLSTRIERASGFVDALTDANMRHASLTIEDKHTNLEQIKEFLQKEIDPD
EKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFDNTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQ
VLDCSVNWKESTF

Specific function: Transcriptional repressor of the kdg operon for pectin utilization [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=335, Percent_Identity=27.1641791044776, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1790194, Length=328, Percent_Identity=27.4390243902439, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1787948, Length=315, Percent_Identity=28.2539682539683, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1790715, Length=325, Percent_Identity=26.4615384615385, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI48994940, Length=318, Percent_Identity=24.5283018867925, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786268, Length=212, Percent_Identity=33.0188679245283, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789068, Length=315, Percent_Identity=22.8571428571429, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1789202, Length=298, Percent_Identity=22.8187919463087, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788474, Length=316, Percent_Identity=23.4177215189873, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787580, Length=324, Percent_Identity=25.6172839506173, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1787906, Length=206, Percent_Identity=26.2135922330097, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790689, Length=331, Percent_Identity=24.1691842900302, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1786540, Length=336, Percent_Identity=24.4047619047619, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012310
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38360; Mature: 38360

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVIHETNYKPSIVARSLNS
CCCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCC
KRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSNYSQESEDRYIESMLLLGV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHCC
DGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWVKTNNYDAVYDMTQSCIEKGY
CCEEECCCHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHEECCCCHHHHHHHHHHHHCCC
EYFLLITADTSRLSTRIERASGFVDALTDANMRHASLTIEDKHTNLEQIKEFLQKEIDPD
EEEEEEEECHHHHHHHHHHHCCHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCC
EKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFDNTEWTCFSSPSVSTLVQPSFEEGQ
CCEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCHHHCCCCHHHHH
QATKILIDQIEGRNQEERQQVLDCSVNWKESTF
HHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVIHETNYKPSIVARSLNS
CCCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCC
KRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSNYSQESEDRYIESMLLLGV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHCC
DGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWVKTNNYDAVYDMTQSCIEKGY
CCEEECCCHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHEECCCCHHHHHHHHHHHHCCC
EYFLLITADTSRLSTRIERASGFVDALTDANMRHASLTIEDKHTNLEQIKEFLQKEIDPD
EEEEEEEECHHHHHHHHHHHCCHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCC
EKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFDNTEWTCFSSPSVSTLVQPSFEEGQ
CCEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCHHHCCCCHHHHH
QATKILIDQIEGRNQEERQQVLDCSVNWKESTF
HHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377 [H]