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The map label for this gene is mraW

Identifier: 116515442

GI number: 116515442

Start: 301027

End: 301977

Strand: Direct

Name: mraW

Synonym: SPD_0304

Alternate gene names: 116515442

Gene position: 301027-301977 (Clockwise)

Preceding gene: 116515692

Following gene: 116516218

Centisome position: 14.71

GC content: 43.85

Gene sequence:

>951_bases
ATGACAAAAGAATTTCATCATGTAACGGTCTTACTCCACGAAACGATTGATATGCTTGACGTAAAGCCTGAAGGTATCTA
CGTTGATGCGACTTTGGGCGGAGCAGGACATAGCGAGTATTTATTAAGTAAATTAAGTGAAAAAGGCCATCTCTATGCCT
TTGACCAGGATCAGAATGCCATTGACAATGCGCAAAAACGCTTGGCACCTTACATTGAGAAGGGAATGGTGACCTTTATC
AAGGATAACTTCCGTCATTTACAGGCACGTTTGCGCGAAGCTGGTGTTCAGGAAATTGATGGAATTTGTTATGACTTGGG
AGTGTCTAGTCCTCAATTGGACCAGCGTGAGCGTGGTTTTTCTTATAAAAAGGATGCGCCACTGGACATGCGGATGAATC
AGGATGCTAGTCTGACAGCCTATGAAGTGGTTAATCATTATGACTATCATGATTTGGTTCGTATTTTCTTCAAATACGGT
GAGGATAAATTCTCTAAACAGATTGCGCGTAAGATTGAGCAAGCGCGTGAAGTGAAGCCGATTGAGACAACGACTGAGTT
AGCAGAGATTATCAAGTTGGTCAAACCTGCCAAGGAACTCAAGAAGAAGGGTCATCCTGCTAAGCAGATTTTCCAGGCTA
TTCGAATTGAAGTCAATGATGAACTGGGAGCGGCAGATGAGTCCATCCAGCAGGCTATGGATATGTTGGCTCTGGATGGT
AGAATTTCAGTGATTACCTTTCATTCCTTAGAAGACCGCTTGACCAAGCAATTGTTCAAGGAAGCTTCAACAGTTGAAGT
TCCAAAAGGCTTGCCTTTCATCCCAGATGATCTCAAGCCCAAGATGGAATTGGTGTCCCGTAAGCCAATCTTGCCAAGTG
CGGAAGAGTTAGAAGCCAATAACCGCTCGCACTCAGCCAAGTTGCGCGTGGTCAGAAAAATTCACAAGTAA

Upstream 100 bases:

>100_bases
TCAAGTATTTTTGACATATTTTTTGAAGAAATAGTAGTCTCCTTGTCCTATTTGTCTGACAAGTGCAAGCTGGTCGGATT
TGTGGTAAAATAGATAAGAT

Downstream 100 bases:

>100_bases
GAGGGAAAAAGATGGCAGAAAAAATGGAAAAAACAGGTCAAATACTACAGATGCAACTTAAACGGTTTTCGCGTGTGGAA
AAAGCTTTTTACTTTTCCAT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MTKEFHHVTVLLHETIDMLDVKPEGIYVDATLGGAGHSEYLLSKLSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFI
KDNFRHLQARLREAGVQEIDGICYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEVNDELGAADESIQQAMDMLALDG
RISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPDDLKPKMELVSRKPILPSAEELEANNRSHSAKLRVVRKIHK

Sequences:

>Translated_316_residues
MTKEFHHVTVLLHETIDMLDVKPEGIYVDATLGGAGHSEYLLSKLSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFI
KDNFRHLQARLREAGVQEIDGICYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEVNDELGAADESIQQAMDMLALDG
RISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPDDLKPKMELVSRKPILPSAEELEANNRSHSAKLRVVRKIHK
>Mature_315_residues
TKEFHHVTVLLHETIDMLDVKPEGIYVDATLGGAGHSEYLLSKLSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIK
DNFRHLQARLREAGVQEIDGICYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYGE
DKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEVNDELGAADESIQQAMDMLALDGR
ISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPDDLKPKMELVSRKPILPSAEELEANNRSHSAKLRVVRKIHK

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=341, Percent_Identity=35.7771260997067, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI165377202, Length=192, Percent_Identity=40.625, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI1786270, Length=319, Percent_Identity=39.4984326018809, Blast_Score=231, Evalue=5e-62,
Organism=Drosophila melanogaster, GI62472493, Length=331, Percent_Identity=36.5558912386707, Blast_Score=174, Evalue=5e-44,
Organism=Drosophila melanogaster, GI28571637, Length=331, Percent_Identity=36.5558912386707, Blast_Score=174, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_STRP2 (Q04MC7)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_815821.1
- ProteinModelPortal:   Q04MC7
- SMR:   Q04MC7
- STRING:   Q04MC7
- EnsemblBacteria:   EBSTRT00000018825
- GeneID:   4441289
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_0304
- eggNOG:   COG0275
- GeneTree:   EBGT00050000027368
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 36066; Mature: 35935

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: BINDING 55-55 BINDING 84-84 BINDING 105-105 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKEFHHVTVLLHETIDMLDVKPEGIYVDATLGGAGHSEYLLSKLSEKGHLYAFDQDQNA
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEECCCCHH
IDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGICYDLGVSSPQLDQRERGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHCCCCCCCCCCHHHCCC
SYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYGEDKFSKQIARKIEQAREVKP
CCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCC
IETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEVNDELGAADESIQQAMDMLALDG
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
RISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPDDLKPKMELVSRKPILPSAEELEAN
CEEEEEEHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHCC
NRSHSAKLRVVRKIHK
CCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TKEFHHVTVLLHETIDMLDVKPEGIYVDATLGGAGHSEYLLSKLSEKGHLYAFDQDQNA
CCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEECCCCHH
IDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGICYDLGVSSPQLDQRERGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHCCCCCCCCCCHHHCCC
SYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYGEDKFSKQIARKIEQAREVKP
CCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCC
IETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEVNDELGAADESIQQAMDMLALDG
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
RISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPDDLKPKMELVSRKPILPSAEELEAN
CEEEEEEHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHCC
NRSHSAKLRVVRKIHK
CCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA