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The map label for this gene is thiM

Identifier: 116515661

GI number: 116515661

Start: 645144

End: 645926

Strand: Direct

Name: thiM

Synonym: SPD_0623

Alternate gene names: 116515661

Gene position: 645144-645926 (Clockwise)

Preceding gene: 116516305

Following gene: 116516536

Centisome position: 31.53

GC content: 36.65

Gene sequence:

>783_bases
ATGACAAGTTTAAAATTATTAAAAGAAAAAGCACCATTGGTCATTTGTATAACCAATGATGTAGTAAAAAATTTCACAGC
AAATGGATTAGTAGCACTGGGCGCCTCACCAGCCATGAGTGAGTTTCCAGCAGATTTAGAGGATTTGTTAAAGTATGCTG
GTGGTTTATTAATAAACATAGGAACATTGACAGATGAAAATTGGAAATTATACCAAGCTGCTCTGAAAATTGCGGAGAAA
TATAATGTCCCAGCAGTTTTAGATCCTGTAGCCTGTGGAGCAGGAGAATATAGAAAAAAAGTAGCAGATGATCTAATCAA
CAATTATAAACTAGCAGCGATTAGAGGAAATGCTGGCGAGATTGCCTCTTTAGTAGGAATAGATGTGGCATCTAAAGGAG
TAGATAGTGCGGGCGTAGATAATATTGACGAAATTGCTCTAGCAGCAAATGAGAAGTTCAATATTCCAATAGTAGTAACA
GGTGAAGTGGATGCCATTGCTGTTAATGGAGAAGTGGTAACGATTCATAATGGTAGTGCTATGATGCCGAAAGTCATTGG
GACAGGATGCTTATTAGGAGCTGTAGTAGCAAGTTTTATCGGACTGGAAAAAGGTCAAGAATTAAAATCATTAGAAACAG
CAATGTTAGTTTACAATATCGCTGGAGAAATGGCAGAAAAACGTCCAAATGGACATCTTCCTGGGACATTTAAAGTTGAA
TTTATAAATGCCTTATACGAGATTACAGATGAAGATGTAAAGGAATTCAAAAGAGTGAAGTAA

Upstream 100 bases:

>100_bases
AGTATAAATCATTCAACGTAAATGAAAAAAATTTCTTAACGAAAGATAGAGATAAATCGAAATCAGTAGATCGTATAAAG
TGAAAAGGAAGGATTTCAAT

Downstream 100 bases:

>100_bases
GATGTTTCATAAAGAATTACTAAAACTATATTTTATTTGTGGAACGACTACTTGCCAAGGAAAAAATCTATATACAGTCG
TTGAGGAAGCCTTAAAAGGT

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase 1; TH kinase 1; Thz kinase 1

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPADLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEK
YNVPAVLDPVACGAGEYRKKVADDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVYNIAGEMAEKRPNGHLPGTFKVE
FINALYEITDEDVKEFKRVK

Sequences:

>Translated_260_residues
MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPADLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEK
YNVPAVLDPVACGAGEYRKKVADDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVYNIAGEMAEKRPNGHLPGTFKVE
FINALYEITDEDVKEFKRVK
>Mature_259_residues
TSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPADLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKY
NVPAVLDPVACGAGEYRKKVADDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVTG
EVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVYNIAGEMAEKRPNGHLPGTFKVEF
INALYEITDEDVKEFKRVK

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=251, Percent_Identity=42.2310756972112, Blast_Score=175, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6325042, Length=272, Percent_Identity=34.1911764705882, Blast_Score=116, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM1_STRP2 (Q04LH9)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816119.1
- ProteinModelPortal:   Q04LH9
- SMR:   Q04LH9
- STRING:   Q04LH9
- EnsemblBacteria:   EBSTRT00000019740
- GeneID:   4441244
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_0623
- eggNOG:   COG2145
- GeneTree:   EBGT00050000026805
- HOGENOM:   HBG351126
- OMA:   AIRGNAG
- ProtClustDB:   PRK09355
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099
- TIGRFAMs:   TIGR00694

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 27607; Mature: 27476

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: BINDING 39-39 BINDING 115-115 BINDING 160-160 BINDING 187-187

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPADLEDLLKYAGGLLINI
CCCHHHHHCCCCEEEEEECHHHHHCCCCCEEEECCCCCHHHCCCCHHHHHHHCCCEEEEE
GTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVADDLINNYKLAAIRGNAGE
ECCCCCCHHHHHHHHHHHHHCCCCEEECCHHCCCHHHHHHHHHHHHCCEEEEEEECCCHH
IASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVTGEVDAIAVNGEVVTIHNGSA
HHHHHHHHHHHCCCCCCCCCCHHHHHEECCCCCCCCEEEECCCEEEEECCEEEEEECCCC
MMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVYNIAGEMAEKRPNGHLPGTFKVE
CCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHH
FINALYEITDEDVKEFKRVK
HHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure 
TSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPADLEDLLKYAGGLLINI
CCHHHHHCCCCEEEEEECHHHHHCCCCCEEEECCCCCHHHCCCCHHHHHHHCCCEEEEE
GTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVADDLINNYKLAAIRGNAGE
ECCCCCCHHHHHHHHHHHHHCCCCEEECCHHCCCHHHHHHHHHHHHCCEEEEEEECCCHH
IASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVTGEVDAIAVNGEVVTIHNGSA
HHHHHHHHHHHCCCCCCCCCCHHHHHEECCCCCCCCEEEECCCEEEEECCEEEEEECCCC
MMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVYNIAGEMAEKRPNGHLPGTFKVE
CCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHH
FINALYEITDEDVKEFKRVK
HHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA