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The map label for this gene is thiE-1 [H]

Identifier: 116516536

GI number: 116516536

Start: 645928

End: 646557

Strand: Direct

Name: thiE-1 [H]

Synonym: SPD_0624

Alternate gene names: 116516536

Gene position: 645928-646557 (Clockwise)

Preceding gene: 116515661

Following gene: 116516079

Centisome position: 31.57

GC content: 33.17

Gene sequence:

>630_bases
ATGTTTCATAAAGAATTACTAAAACTATATTTTATTTGTGGAACGACTACTTGCCAAGGAAAAAATCTATATACAGTCGT
TGAGGAAGCCTTAAAAGGTGGTATAACCTTATTTCAATTTCGAGAAAAAGGTGAGAGTGCTTTAGAAGGCTTAGAAAAAC
TTGAGCTAGCTATCCAAATTAAAGAACTTTGTAAAAAATACAATGTTCCATTTATCGTTAATGACGATATAGATTTAGCA
ATGGAAATTGACGCCGATGGCGTACATGTAGGTCAAGATGATATTGGTGTTGATGAAATTAGAAAATTGATGCCAGATAA
AATAATTGGTCTTTCTATAAGAAACGAAGAAGAATTTCAACAATCAAAAGTTGAATATGTAGATTATGTAGGTGTTGGGC
CTGTATTTGATACCCAGTCAAAAGATGATGCTGGTGGTGCTATAGGTTATGAAGGTCTTGAATTGATGAGAAAACTATTG
CCACAAATGCCTTTAGTTGCAATTGGTGGGATACAGACGAAACATATTAAAGACATTATAAAGACCAATATGGATGGCGT
TTCAATCATTTCGGCAATATCGTATGCAAAAAATATAGAAAAAACTGTTCGGGAAATGAGTGAACAATAG

Upstream 100 bases:

>100_bases
CCAAATGGACATCTTCCTGGGACATTTAAAGTTGAATTTATAAATGCCTTATACGAGATTACAGATGAAGATGTAAAGGA
ATTCAAAAGAGTGAAGTAAG

Downstream 100 bases:

>100_bases
ATATCTATCCTTGGATTCTAAGTAAGTAGGATTTTCCTTTTTTAAAAAGCTATGTCCAGATTCAGCTTGAATGGCTAATT
CATCAAACAGGTCATTTGTT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase 1; TMP-PPase 1; Thiamine-phosphate synthase 1 [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKGESALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLA
MEIDADGVHVGQDDIGVDEIRKLMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ

Sequences:

>Translated_209_residues
MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKGESALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLA
MEIDADGVHVGQDDIGVDEIRKLMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ
>Mature_209_residues
MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKGESALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLA
MEIDADGVHVGQDDIGVDEIRKLMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=195, Percent_Identity=28.2051282051282, Blast_Score=74, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6325042, Length=229, Percent_Identity=35.8078602620087, Blast_Score=122, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 23334; Mature: 23334

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKGESALEGLEKLELAIQI
CCHHHHHHHHHHHCCCEECCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHH
KELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRKLMPDKIIGLSIRNEEEFQ
HHHHHHCCCCEEECCCCCEEEEECCCCEEECCCCCCHHHHHHHCCHHEEEEEECCHHHHH
QSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLLPQMPLVAIGGIQTKHIKDII
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHH
KTNMDGVSIISAISYAKNIEKTVREMSEQ
HCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKGESALEGLEKLELAIQI
CCHHHHHHHHHHHCCCEECCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHH
KELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRKLMPDKIIGLSIRNEEEFQ
HHHHHHCCCCEEECCCCCEEEEECCCCEEECCCCCCHHHHHHHCCHHEEEEEECCHHHHH
QSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLLPQMPLVAIGGIQTKHIKDII
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHH
KTNMDGVSIISAISYAKNIEKTVREMSEQ
HCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]