Click here to switch to the map view.

The map label for this gene is thiE

Identifier: 116515649

GI number: 116515649

Start: 651741

End: 652373

Strand: Direct

Name: thiE

Synonym: SPD_0631

Alternate gene names: 116515649

Gene position: 651741-652373 (Clockwise)

Preceding gene: 116515654

Following gene: 116515476

Centisome position: 31.85

GC content: 42.97

Gene sequence:

>633_bases
ATGAATAGAGAAGCACTTAGACTATATCTGGTAACCAATCGCTATCAAGATTCCGTGGAAAGCTTTCTTGCAAAAGTTGA
GACGGCCTGCCGTTCAGGGGTTACCATAGTCCAATTGCGAGAAAAAAATCTCACAACCAATCAATATTATCAACTGGCAA
AACAAGTCAAGGAAATAACAGATGCTTATCAGGTACCCTTGATAATCGATGATCGGTTGGATGTTTGTCTTGCGGTTGAT
GCAGCAGGTCTGCATATCGGAGATGATGAATTACCAGTTTCGGTAGCTCGCAAAGTCTTGGGTCCTGAAAAAATCCTCGG
TGTCACCGCTAAAACGGTTAAAAGAGCTCTGGAAGCGGAGAAATCAGGTGCAGATTACTTGGGTACAGGAGCCATTTTCC
CAACCACCACCAAGGAAAATGCGCCCATTACTCTGATATCGACCTTGAAAACAATTTGCCAAACGGTTGCCATTCCAGTA
GTTGCTATTGGAGGCTTGACTTCGGAGAATATTGATCAGCTTATGGGCACTGGCATAGCTGGTGTAGCTGTTGTACGTGA
TTTGATGCAAGCAGAAGATATTGAGGCAAAAACGCAAGCCTTTTTAAAAAAGTTGCATAACATTCTTTCCTAA

Upstream 100 bases:

>100_bases
CAAGGAATGGGATTGGAAGAATTTCGTTACCAAGTACTCAATCAGCTTTCGCTCCTAAGAAGAGATGAAAATTGGCTAGA
TACCATCAAAGGAGAGGTTT

Downstream 100 bases:

>100_bases
TTAATACTCAATGAAAATCAAAGAGTAAACTAGAAAGCTAGTCGCAGGTTGCTCAAAGTACTGCCTTGAGGTTTTAGATA
AGACTGACGAAGTCAGCTCA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase 2; TMP-PPase 2; Thiamine-phosphate synthase 2

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNLTTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVD
AAGLHIGDDELPVSVARKVLGPEKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLKKLHNILS

Sequences:

>Translated_210_residues
MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNLTTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVD
AAGLHIGDDELPVSVARKVLGPEKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLKKLHNILS
>Mature_210_residues
MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNLTTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVD
AAGLHIGDDELPVSVARKVLGPEKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLKKLHNILS

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Saccharomyces cerevisiae, GI6325042, Length=227, Percent_Identity=36.1233480176211, Blast_Score=132, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE2_STRPN (P66920)

Other databases:

- EMBL:   AE005672
- PIR:   A95084
- RefSeq:   NP_345226.1
- ProteinModelPortal:   P66920
- SMR:   P66920
- EnsemblBacteria:   EBSTRT00000027247
- GeneID:   930675
- GenomeReviews:   AE005672_GR
- KEGG:   spn:SP_0725
- TIGR:   SP_0725
- GeneTree:   EBGT00050000028192
- HOGENOM:   HBG754477
- OMA:   SAICHAE
- ProtClustDB:   PRK00043
- BioCyc:   SPNE170187-1:SP_0725-MONOMER
- BRENDA:   2.5.1.3
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 22771; Mature: 22771

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNLTTNQYYQLAKQVKEIT
CCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHH
DAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGPEKILGVTAKTVKRALEAE
HHHHCCEEECCCCCEEEEEECCCCEECCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHH
KSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPVVAIGGLTSENIDQLMGTGIA
HCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHH
GVAVVRDLMQAEDIEAKTQAFLKKLHNILS
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNLTTNQYYQLAKQVKEIT
CCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHH
DAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGPEKILGVTAKTVKRALEAE
HHHHCCEEECCCCCEEEEEECCCCEECCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHH
KSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPVVAIGGLTSENIDQLMGTGIA
HCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHH
GVAVVRDLMQAEDIEAKTQAFLKKLHNILS
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916