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The map label for this gene is nanA [H]

Identifier: 116515419

GI number: 116515419

Start: 1190890

End: 1191621

Strand: Reverse

Name: nanA [H]

Synonym: SPD_1163

Alternate gene names: 116515419

Gene position: 1191621-1190890 (Counterclockwise)

Preceding gene: 116516518

Following gene: 116516734

Centisome position: 58.24

GC content: 43.31

Gene sequence:

>732_bases
TTGATTTTGGAAGAAGTCATGGCGGTAGCCAAAGGTAAATTGACCATTATTGCCCATGTTGCTTGCAATAATACTAAAGA
TAGTATGGAACTTGCTCGCCATGCTGAAAGCTTGGGAGTAGATGCTATTGCAACGATTCCACCAATTTACTTCCGCTTGC
CAGAATACTCAGTTGCCAAATACTGGAACGATATCAGTTCTGCAGCTCCAAACACAGACTACGTGATTTACAACATTCCT
CAATTGGCAGGGGTTGCTTTGACTCCAAGCCTTTACACAGAAATGTTGAAAAATCCTCGTGTTATCGGTGTTAAGAACTC
TTCTATGCCAGTTCAAGATATCCAAACCTTTGTCAGCCTTGGTGGGGAAGACCATATCGTCTTTAATGGTCCTGATGAGC
AGTTTCTAGGAGGACGCCTCATGGGTGCTAGGGCTGGTATCGGTGGTACTTATGGTGCTATGCCAGAACTCTTCTTGAAA
CTCAATCAGTTGATTGCGGATAAGGACCTAGAAACAGCGCGTGAATTGCAGTATGCTATCAACGCAATCATTGGTAAACT
CACTTCTGTTCATGGAAATATGTACGGTGTCATCAAAGAAGTTTTGAAAATCAATGAAGGCTTGACTATTGGATCTGTTC
GTTCACCATTGACACCAGTGACTGAAGAAGATCGTCCAGTTGTAGAAGAAGCTGCTGCCTTGATTCGTGAAACCAAGGAG
CGCTTCCTCTAA

Upstream 100 bases:

>100_bases
GCGTGCCTAGGTTCAATACTTCATTGATAAAGGTGTTCAAGGTCTTTATGTCAATGGTTCTTCTGGTGAATGTATCTACC
AAAGCGTTGAAGATCGCAAG

Downstream 100 bases:

>100_bases
TCCATAAGGAGGTATTTATGACGCACTACGTTGCCAATCCAGAGGTGGTCATTCTCGAGGGTGGCATCATGGGACAAGAA
GCTATCCTCAAACCAAAGAT

Product: N-acetylneuraminate lyase

Products: NA

Alternate protein names: N-acetylneuraminate pyruvate-lyase; N-acetylneuraminic acid aldolase; Sialate lyase; Sialic acid aldolase; Sialic acid lyase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGVDAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIP
QLAGVALTPSLYTEMLKNPRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPELFLK
LNQLIADKDLETARELQYAINAIIGKLTSVHGNMYGVIKEVLKINEGLTIGSVRSPLTPVTEEDRPVVEEAAALIRETKE
RFL

Sequences:

>Translated_243_residues
MILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGVDAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIP
QLAGVALTPSLYTEMLKNPRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPELFLK
LNQLIADKDLETARELQYAINAIIGKLTSVHGNMYGVIKEVLKINEGLTIGSVRSPLTPVTEEDRPVVEEAAALIRETKE
RFL
>Mature_243_residues
MILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGVDAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIP
QLAGVALTPSLYTEMLKNPRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPELFLK
LNQLIADKDLETARELQYAINAIIGKLTSVHGNMYGVIKEVLKINEGLTIGSVRSPLTPVTEEDRPVVEEAAALIRETKE
RFL

Specific function: Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate [H]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family. NanA subfamily [H]

Homologues:

Organism=Homo sapiens, GI13540533, Length=235, Percent_Identity=29.3617021276596, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1789620, Length=238, Percent_Identity=30.2521008403361, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI87082415, Length=177, Percent_Identity=27.1186440677966, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786463, Length=206, Percent_Identity=25.2427184466019, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788823, Length=232, Percent_Identity=21.551724137931, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR005264 [H]

Pfam domain/function: PF00701 DHDPS [H]

EC number: =4.1.3.3 [H]

Molecular weight: Translated: 26517; Mature: 26517

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGVDAIATIPPIYFRLPEYSVAK
CCHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCHHHHCCCCEEECCCHHHHH
YWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKNPRVIGVKNSSMPVQDIQTFVSL
HHHHHHHCCCCCCEEEEECHHHCCEEECHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHC
GGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPELFLKLNQLIADKDLETARELQYAI
CCCCEEEECCCCHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
NAIIGKLTSVHGNMYGVIKEVLKINEGLTIGSVRSPLTPVTEEDRPVVEEAAALIRETKE
HHHHHHHHHHCCHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
RFL
HCC
>Mature Secondary Structure
MILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGVDAIATIPPIYFRLPEYSVAK
CCHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCHHHHCCCCEEECCCHHHHH
YWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKNPRVIGVKNSSMPVQDIQTFVSL
HHHHHHHCCCCCCEEEEECHHHCCEEECHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHC
GGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPELFLKLNQLIADKDLETARELQYAI
CCCCEEEECCCCHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
NAIIGKLTSVHGNMYGVIKEVLKINEGLTIGSVRSPLTPVTEEDRPVVEEAAALIRETKE
HHHHHHHHHHCCHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
RFL
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]