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The map label for this gene is carA [H]

Identifier: 116515374

GI number: 116515374

Start: 1165769

End: 1166848

Strand: Reverse

Name: carA [H]

Synonym: SPD_1132

Alternate gene names: 116515374

Gene position: 1166848-1165769 (Counterclockwise)

Preceding gene: 116516819

Following gene: 116516501

Centisome position: 57.03

GC content: 44.17

Gene sequence:

>1080_bases
ATGACAAAAAGAATTCTAGTATTAGAAGATGGCACAGTTTTTGAAGGCAAGGCCTTCGGAGCAGATATTGATGTAACAGG
CGAAATCGTCTTTAATACAGGGATGACCGGCTACCAAGAATCCATTACAGACCAGTCTTATAATGGACAAATCTTGACCT
TTACTTATCCTTTGGTAGGAAATTATGGTATTAATCGTGATGATTATGAATCCATTATTCCAACTTGTAAGGGAGTCGTT
GTTTTTGAAGAAGCGCGTAGAGCTAGCAACTGGCGCAACCAAATGACCTTGGATGAATTTTTGAAAGCCAAGAAAATTCC
AGGGATTTCAGGAATTGATACGCGTGCTCTTACCAAGATTATCCGTAAGCATGGTACTATGCGTGCAACCTTGACCCATG
TTGGGGACAGTATGGACCATGTGACGGACCAGCTCCAAGCAACAGTTTTACCGACAGACAATATCAAACAGGTTTCTACT
AAAACGTCTTATCCAGCTCCAGGAGTTGGTTTGAGCGTGGTGCTAGTGGACTTTGGTCTCAAGCACTCAATCCTACGTGA
ACTTTCTAAACGCAACTGTAACGTGACAGTTGTTCCTTATTCGACAACGGCTGAAGAAATTCTCCATCTCAATCCTGACG
GAGTTATGTTGTCAAATGGTCCAGGTAACCCAGAAGACGTTCCACAAGCACTCGACATGATTCGCGGGGGACAAGGAAAA
ATTCCAATCTTTGGTATTTGTATGGGACACCAACTCTTTGCAATGGCAAACGGGGCTAAGACCTATAAGATGAAGTTTGG
CCACCGTGGATTCAACCATGCGGTACGTGAAATCGCAACAGGACGAGTAGACTTCACCAGCCAGAACCATGGTTATGCAG
TCAGCCGCGAGGATTTGCCAGAGCACTTGATTATTACCCACGAAGAAATCAATGACAAGTCAGTGGAAGGTGTGCGCCAC
AGATACCAACCTGCTTTCTCTGTTCAATACCACCCAGATGCAGCTCCTGGGCCACACGACGCTAGCTACCTATTTGACGA
GTTTATCGAGATGATGGAAGTTTTTAAACAATCAAACTAA

Upstream 100 bases:

>100_bases
GTTCGAATGGCAATCTTAGAATCCGTACTAGCGAGTAGAAACGCCAACTAAAACACAAGACGTTAAAAGACGCCAGTGAG
CGTCCACGAGAGGTGAAAAT

Downstream 100 bases:

>100_bases
GAACGAGTAAAAGCTCGTCGGAGAATTTATGTAACTGAACACGGACGGAAAGCTCAGAATGTAGAGAAACCAACTTGGAT
TGTCAATCCTTCCTTGATTT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITDQSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVV
VFEEARRASNWRNQMTLDEFLKAKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLSNGPGNPEDVPQALDMIRGGQGK
IPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVREIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRH
RYQPAFSVQYHPDAAPGPHDASYLFDEFIEMMEVFKQSN

Sequences:

>Translated_359_residues
MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITDQSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVV
VFEEARRASNWRNQMTLDEFLKAKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLSNGPGNPEDVPQALDMIRGGQGK
IPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVREIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRH
RYQPAFSVQYHPDAAPGPHDASYLFDEFIEMMEVFKQSN
>Mature_358_residues
TKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITDQSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVV
FEEARRASNWRNQMTLDEFLKAKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVSTK
TSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLSNGPGNPEDVPQALDMIRGGQGKI
PIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVREIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHR
YQPAFSVQYHPDAAPGPHDASYLFDEFIEMMEVFKQSN

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=362, Percent_Identity=40.3314917127072, Blast_Score=262, Evalue=4e-70,
Organism=Homo sapiens, GI21361331, Length=362, Percent_Identity=38.121546961326, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI169790915, Length=362, Percent_Identity=38.121546961326, Blast_Score=234, Evalue=1e-61,
Organism=Escherichia coli, GI1786215, Length=376, Percent_Identity=43.8829787234042, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1789760, Length=140, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=369, Percent_Identity=40.650406504065, Blast_Score=252, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6324878, Length=376, Percent_Identity=40.1595744680851, Blast_Score=258, Evalue=7e-70,
Organism=Saccharomyces cerevisiae, GI6322331, Length=394, Percent_Identity=37.5634517766497, Blast_Score=244, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6322638, Length=147, Percent_Identity=34.0136054421769, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45555749, Length=375, Percent_Identity=42.1333333333333, Blast_Score=269, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24642586, Length=375, Percent_Identity=42.1333333333333, Blast_Score=269, Evalue=2e-72,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 39822; Mature: 39690

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITDQSYNGQILTFTYPLVG
CCCEEEEEECCCEEECCCCCCCCEEEEEEEEECCCCCHHHHHCCCCCCCEEEEEEECCCC
NYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLKAKKIPGISGIDTRALTKI
CCCCCCHHHHHHHHHHCCEEEEHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHH
IRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVSTKTSYPAPGVGLSVVLVDFGL
HHHCCCEEEEHHHHCCHHHHHHHHHEEEECCCCCCHHHHCCCCCCCCCCCCEEEEEHHCH
KHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLSNGPGNPEDVPQALDMIRGGQGK
HHHHHHHHHHCCCCEEEEECCCCHHHHEEECCCCEEEECCCCCHHHHHHHHHHHCCCCCC
IPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVREIATGRVDFTSQNHGYAVSREDLP
CCEEEEECCCEEEEECCCCEEEEEECCCCCHHHHHHHHHCCCEEECCCCCCEEECCCCCC
EHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDAAPGPHDASYLFDEFIEMMEVFKQSN
CEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITDQSYNGQILTFTYPLVG
CCEEEEEECCCEEECCCCCCCCEEEEEEEEECCCCCHHHHHCCCCCCCEEEEEEECCCC
NYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLKAKKIPGISGIDTRALTKI
CCCCCCHHHHHHHHHHCCEEEEHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHH
IRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVSTKTSYPAPGVGLSVVLVDFGL
HHHCCCEEEEHHHHCCHHHHHHHHHEEEECCCCCCHHHHCCCCCCCCCCCCEEEEEHHCH
KHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLSNGPGNPEDVPQALDMIRGGQGK
HHHHHHHHHHCCCCEEEEECCCCHHHHEEECCCCEEEECCCCCHHHHHHHHHHHCCCCCC
IPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVREIATGRVDFTSQNHGYAVSREDLP
CCEEEEECCCEEEEECCCCEEEEEECCCCCHHHHHHHHHCCCEEECCCCCCEEECCCCCC
EHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDAAPGPHDASYLFDEFIEMMEVFKQSN
CEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]