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The map label for this gene is pyrB

Identifier: 116516819

GI number: 116516819

Start: 1166898

End: 1167821

Strand: Reverse

Name: pyrB

Synonym: SPD_1133

Alternate gene names: 116516819

Gene position: 1167821-1166898 (Counterclockwise)

Preceding gene: 116515708

Following gene: 116515374

Centisome position: 57.08

GC content: 42.64

Gene sequence:

>924_bases
ATGTCAGAAAATCAACAAGCATTGAACCATGTGGTGTCCATGGAAGACCTCACTGTTGATCAAGTAATGAAATTGATCAA
GCGAGGAATTGAGTTTAAAAATGGAGCCCAGATTCCCTATGAAGACCATCCGATTGTTTCCAATCTCTTCTTTGAGGATT
CTACACGGACACATAAGTCCTTTGAAGTCGCAGAGATTAAACTTGGATTGGAACGACTTGACTTTGATGTGAAGACTAGT
TCGGTTAATAAGGGTGAGACACTTTATGATACCATTTTGACTCTGTCTGCTTTAGGAGTGGATGTCTGTGTGATTCGCCA
TCCAGAGGTCGACTACTATAGAGAATTGATTGCTAGTCCAACGATTACGACTTCCATCATCAATGGTGGAGATGGTTCGG
GCCAACACCCTAGCCAGAGCTTGCTTGATTTGATGACCATTTATGAGGAATTTGGCCACTTTGAGGGTCTTAAAGTTGCT
ATTGCAGGTGACTTGGACCACTCACGCGTTGCCAAATCCAATATGCAGATTTTGAAACGCTTGGGATCTGAACTCTTTTT
CGCTGGACCTGAGGAATGGAGAAGTCAAGAGTTTGCAGACTATGGAAAGTTTGTAACCATTGATGAAATCATTGATCAGG
TTGATGTCATGATGTTTCTCCGTGTGCAACACGAACGCCATGATAGTGGAGCTGTATTTTCAAAAGAAGACTACCATGCC
CAACATGGCTTGACTCAAGAACGTTACGATCGTTTGAAAGAAACAGCAATCCTCATGCACCCAGCTCCAATCAATCGTGA
TGTAGAAATAGCAGACCACTTGGTTGAAGCACCAAAATCACGGATTGTCCAACAAATGACCAATGGTGTCTTTGTTCGAA
TGGCAATCTTAGAATCCGTACTAGCGAGTAGAAACGCCAACTAA

Upstream 100 bases:

>100_bases
AACCAGTCGTTCTGAAGAAATCATCGTAGAGATGACAGAACTTGATGACCAAGACAGAGTTCTGATTACTGAAGAAGCTT
AGAAAGTTAAAGGAGTAGCC

Downstream 100 bases:

>100_bases
AACACAAGACGTTAAAAGACGCCAGTGAGCGTCCACGAGAGGTGAAAATATGACAAAAAGAATTCTAGTATTAGAAGATG
GCACAGTTTTTGAAGGCAAG

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQIPYEDHPIVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTS
SVNKGETLYDTILTLSALGVDVCVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
IAGDLDHSRVAKSNMQILKRLGSELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMMFLRVQHERHDSGAVFSKEDYHA
QHGLTQERYDRLKETAILMHPAPINRDVEIADHLVEAPKSRIVQQMTNGVFVRMAILESVLASRNAN

Sequences:

>Translated_307_residues
MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQIPYEDHPIVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTS
SVNKGETLYDTILTLSALGVDVCVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
IAGDLDHSRVAKSNMQILKRLGSELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMMFLRVQHERHDSGAVFSKEDYHA
QHGLTQERYDRLKETAILMHPAPINRDVEIADHLVEAPKSRIVQQMTNGVFVRMAILESVLASRNAN
>Mature_306_residues
SENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQIPYEDHPIVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSS
VNKGETLYDTILTLSALGVDVCVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVAI
AGDLDHSRVAKSNMQILKRLGSELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMMFLRVQHERHDSGAVFSKEDYHAQ
HGLTQERYDRLKETAILMHPAPINRDVEIADHLVEAPKSRIVQQMTNGVFVRMAILESVLASRNAN

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=318, Percent_Identity=32.3899371069182, Blast_Score=152, Evalue=5e-37,
Organism=Escherichia coli, GI2367364, Length=304, Percent_Identity=33.5526315789474, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI193204318, Length=326, Percent_Identity=33.1288343558282, Blast_Score=126, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6322331, Length=273, Percent_Identity=35.1648351648352, Blast_Score=129, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24642586, Length=275, Percent_Identity=34.1818181818182, Blast_Score=141, Evalue=6e-34,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_STRP2 (Q04K46)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816602.1
- ProteinModelPortal:   Q04K46
- SMR:   Q04K46
- STRING:   Q04K46
- EnsemblBacteria:   EBSTRT00000020483
- GeneID:   4442176
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1133
- eggNOG:   COG0540
- GeneTree:   EBGT00050000027081
- HOGENOM:   HBG579429
- OMA:   LYFAGPE
- ProtClustDB:   PRK00856
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34707; Mature: 34576

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQIPYEDHPIVSNLFFEDSTRTHKS
CCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHEECCCCCCCCC
FEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDVCVIRHPEVDYYRELIASP
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHCCC
TITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVAIAGDLDHSRVAKSNMQILKR
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHH
LGSELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMMFLRVQHERHDSGAVFSKEDYHA
HCHHHEECCHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
QHGLTQERYDRLKETAILMHPAPINRDVEIADHLVEAPKSRIVQQMTNGVFVRMAILESV
HHCCCHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHH
LASRNAN
HHHCCCC
>Mature Secondary Structure 
SENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQIPYEDHPIVSNLFFEDSTRTHKS
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHEECCCCCCCCC
FEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDVCVIRHPEVDYYRELIASP
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHCCC
TITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVAIAGDLDHSRVAKSNMQILKR
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHH
LGSELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMMFLRVQHERHDSGAVFSKEDYHA
HCHHHEECCHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
QHGLTQERYDRLKETAILMHPAPINRDVEIADHLVEAPKSRIVQQMTNGVFVRMAILESV
HHCCCHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHH
LASRNAN
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA