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The map label for this gene is ybjI [H]

Identifier: 116493530

GI number: 116493530

Start: 1781385

End: 1782206

Strand: Reverse

Name: ybjI [H]

Synonym: PEPE_1803

Alternate gene names: 116493530

Gene position: 1782206-1781385 (Counterclockwise)

Preceding gene: 116493531

Following gene: 116493529

Centisome position: 97.26

GC content: 40.75

Gene sequence:

>822_bases
ATGGGTATTCAATTAATTGCAAGTGATATCGACGGAACTTTTCTAAATGATCAACAGGATTTTGACCATCAACGTTTTCA
AGCTCAGTTAGACAAACTTAATGAACGAAAAATTAAATTTGTAGTAGCGAGTGGAAATCAGATGGCTCATTGTCAGGAAG
TATTTGCTGGCATTAAGGGTGACTTAACCTTTGTTGCCGAAGATGGTGCCCTTACGATTGAACAAGGGAAAATTTTAGAC
GATAATCCGCTAAAACCGGCTTTATTACACGACACAATTGAGTACTTATTGACGACTCCGATGTTTAAAGGCGTTGAGCT
GATTCTGTCGAGCCAACATCGTGCATTTACTAATATGAAAATGGATAATCCGGAATGGCCCGTTAGCTCGTATTTTTACA
GCCAATTAACCCCAGTGGATCACTTTAAAGACATTAAAGATCCAATTTATAAGATTGATGCCCATTGGGTTGATATTGAT
AATGTGCGTGAACAAGCCGCACTGTTAAAAACCGCTTTAGGGGATAGCTTGGGAAGTGTGATGAGTGGCTTCAATGGAAT
TGACATTACGATGCCCCATGTTACTAAAGCGTATGGACTTCAGCAATTACAAAATCGTTGGAAAGTTTCGATGGATGAAA
CGATGGCTTTTGGTGATACCCAAAATGACGAAGCCATGTTGCATCATGCGCGACTAGGCTATGCAATGAAAAACGCAGAA
CAAGAAGCACTGGACGCCACCGAATTGGTTACACCATTGGATAACAACGATAGTGGAGTGATGGACATGATTGATCGGGT
GCTCCGCGGTGAAATTGAATAA

Upstream 100 bases:

>100_bases
TGGGGCTACAATGACAAAATGGAAGTTGGTAAAAAGGTTGAAAAACCAATTAACCCTAGTAAAAAATAATTTGTAAAAAG
CTAATATGTAGGAGGCCACC

Downstream 100 bases:

>100_bases
TTGATAGATTGTTCTTGGTTTATGGACCGCTACTAGGATGGAATGCAATTTTCCATATAGTAGTGGTTTTTTGTATGGAA
ATGGGTTTCAGGTTTACGTG

Product: HAD superfamily hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MGIQLIASDIDGTFLNDQQDFDHQRFQAQLDKLNERKIKFVVASGNQMAHCQEVFAGIKGDLTFVAEDGALTIEQGKILD
DNPLKPALLHDTIEYLLTTPMFKGVELILSSQHRAFTNMKMDNPEWPVSSYFYSQLTPVDHFKDIKDPIYKIDAHWVDID
NVREQAALLKTALGDSLGSVMSGFNGIDITMPHVTKAYGLQQLQNRWKVSMDETMAFGDTQNDEAMLHHARLGYAMKNAE
QEALDATELVTPLDNNDSGVMDMIDRVLRGEIE

Sequences:

>Translated_273_residues
MGIQLIASDIDGTFLNDQQDFDHQRFQAQLDKLNERKIKFVVASGNQMAHCQEVFAGIKGDLTFVAEDGALTIEQGKILD
DNPLKPALLHDTIEYLLTTPMFKGVELILSSQHRAFTNMKMDNPEWPVSSYFYSQLTPVDHFKDIKDPIYKIDAHWVDID
NVREQAALLKTALGDSLGSVMSGFNGIDITMPHVTKAYGLQQLQNRWKVSMDETMAFGDTQNDEAMLHHARLGYAMKNAE
QEALDATELVTPLDNNDSGVMDMIDRVLRGEIE
>Mature_272_residues
GIQLIASDIDGTFLNDQQDFDHQRFQAQLDKLNERKIKFVVASGNQMAHCQEVFAGIKGDLTFVAEDGALTIEQGKILDD
NPLKPALLHDTIEYLLTTPMFKGVELILSSQHRAFTNMKMDNPEWPVSSYFYSQLTPVDHFKDIKDPIYKIDAHWVDIDN
VREQAALLKTALGDSLGSVMSGFNGIDITMPHVTKAYGLQQLQNRWKVSMDETMAFGDTQNDEAMLHHARLGYAMKNAEQ
EALDATELVTPLDNNDSGVMDMIDRVLRGEIE

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates FMN and beta-glucose 1-phosphate [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081790, Length=271, Percent_Identity=36.90036900369, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI1787043, Length=274, Percent_Identity=35.7664233576642, Blast_Score=143, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 30722; Mature: 30591

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIQLIASDIDGTFLNDQQDFDHQRFQAQLDKLNERKIKFVVASGNQMAHCQEVFAGIKG
CCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCC
DLTFVAEDGALTIEQGKILDDNPLKPALLHDTIEYLLTTPMFKGVELILSSQHRAFTNMK
CEEEEECCCEEEEECCEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHCCC
MDNPEWPVSSYFYSQLTPVDHFKDIKDPIYKIDAHWVDIDNVREQAALLKTALGDSLGSV
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHEECCCEEECHHHHHHHHHHHHHHHHHHHHHH
MSGFNGIDITMPHVTKAYGLQQLQNRWKVSMDETMAFGDTQNDEAMLHHARLGYAMKNAE
HCCCCCCEEECCHHHHHHHHHHHHHHHCCCHHHHEECCCCCCCHHHHHHHHHHHHHHCCH
QEALDATELVTPLDNNDSGVMDMIDRVLRGEIE
HHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
GIQLIASDIDGTFLNDQQDFDHQRFQAQLDKLNERKIKFVVASGNQMAHCQEVFAGIKG
CCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCC
DLTFVAEDGALTIEQGKILDDNPLKPALLHDTIEYLLTTPMFKGVELILSSQHRAFTNMK
CEEEEECCCEEEEECCEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHCCC
MDNPEWPVSSYFYSQLTPVDHFKDIKDPIYKIDAHWVDIDNVREQAALLKTALGDSLGSV
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHEECCCEEECHHHHHHHHHHHHHHHHHHHHHH
MSGFNGIDITMPHVTKAYGLQQLQNRWKVSMDETMAFGDTQNDEAMLHHARLGYAMKNAE
HCCCCCCEEECCHHHHHHHHHHHHHHHCCCHHHHEECCCCCCCHHHHHHHHHHHHHHCCH
QEALDATELVTPLDNNDSGVMDMIDRVLRGEIE
HHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]