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The map label for this gene is yieO [C]

Identifier: 116493529

GI number: 116493529

Start: 1779843

End: 1781165

Strand: Reverse

Name: yieO [C]

Synonym: PEPE_1802

Alternate gene names: 116493529

Gene position: 1781165-1779843 (Counterclockwise)

Preceding gene: 116493530

Following gene: 116493528

Centisome position: 97.2

GC content: 37.79

Gene sequence:

>1323_bases
ATGAACAAACAGAAATTTGGTATGATTTTACCAATTATTTTAATTAGTTATTTTATGATTCTTTTGGATAATTCAATTGT
TTTTACAAGTACGGTAAAGATTGCAGCAGACTTGAGTTTGTCGGCTTCGGAATTATCGTGGGTAACTAATGCATATGCAT
TAACCTTTGGTGGATTATTAATGATTGGTGGACGGTCAGGAGATATTTTTGGTCGAAGAAATGTCTTTTTAATCGGGTTG
GTAATCTTTAGTATAGGATCCTTATTGGTTGGTTTATCAACTAATGGTTTAATGATTATCGCCATGCGAGCCTTACAAGG
AATTGGATCGGCAATTCTTGCTCCAACCACTTTAGCTTTGTTAATGGATAGTTATCGAGATCAGATGAGAACCAGAGCGA
TTGTATATTATGGTGCTACTGGTGGATTAGGTGCCAGCATTGGATTAGTTGTTGGTGGATTGATTGCTAGTTACGCTAGT
TGGCGCTGGGGCTTCTTACTTAACGTGCCCGTAGGGATACTGATGATTGTGTTAACACTCAAATATATTCCAGCAAGTAA
AAAGGTAGAAGGAAAATTAGATTGGTTAGGTTCGATTTTGTCAGTCTTAGGAATTACTGCCCTTGTTTATGGAATTAATG
GAGCTAGTCATCCGGTACCCATGGTTATGACCGGGATTTTACTTTTGGTTTTATTTGTATGGCAAGAAAAAGTTTCTAAG
CACCCGGTTACACCATTAAAACTATTTAAAGATCGAGAAAGAAGTAGTGCTTATATTGCAAGGTTCTTTTTCTTAGGTGC
AATGATTTCATACTTCTTCTTAACGCCACAAGCGATGCAACAGGTTTATCACTTTACGCCATTAATGGCAGCGGTAGGAT
TCTTACCAGAAACACTACCTCAATTTGTTTTTGCTACATTGGTATCACGGTTAAACGAAAAGTTTACTAATACTCAGATT
TTAATTGCTGGAACGGTAATTACCTTAGCTGGATTGGTTTTCGCCGCTGTGATAGGGATACAAGCAGGATATTGGTGGTC
AGTAGCTATTCCAATGGTGATTATTGGTATAGGTCAAGGATTTACATTGAGCCCTCTAACCGTAGCCGGAGTAGCAAATA
CGGATAAGGAAATTGCCGGATCGGCTTCAGGTGTAGTAAATATGGTTCATCAAATTGGTAGTTCAGTGGGACTTTCAATT
ATCACCGCGCTGACAGCTAATTTAATTAGCCCAGTATTATCATATGACCACGCTATCATCATTATGGCCGTATTTATGTT
GATTTCAGTCGGAGCTACTTTAAATATCGGTCTTAAAAAATAA

Upstream 100 bases:

>100_bases
TAAGAAAAACTTATGAAAATTGGTAAGAATCGAAATTGGTTAAGAATGGGGGAGCTTGTAGAATAATTACATAATAAAAA
TTTGGATGCGGGTGATAGGA

Downstream 100 bases:

>100_bases
GTGATTTTATAAAGAGAGGGATTATCATGAACGTATTAATTATTGGCGCAACAGGAACGATTGGAAATGCAGTAAGACAA
ACTTTATTAAGTGAAACAGA

Product: major facilitator superfamily permease

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MNKQKFGMILPIILISYFMILLDNSIVFTSTVKIAADLSLSASELSWVTNAYALTFGGLLMIGGRSGDIFGRRNVFLIGL
VIFSIGSLLVGLSTNGLMIIAMRALQGIGSAILAPTTLALLMDSYRDQMRTRAIVYYGATGGLGASIGLVVGGLIASYAS
WRWGFLLNVPVGILMIVLTLKYIPASKKVEGKLDWLGSILSVLGITALVYGINGASHPVPMVMTGILLLVLFVWQEKVSK
HPVTPLKLFKDRERSSAYIARFFFLGAMISYFFLTPQAMQQVYHFTPLMAAVGFLPETLPQFVFATLVSRLNEKFTNTQI
LIAGTVITLAGLVFAAVIGIQAGYWWSVAIPMVIIGIGQGFTLSPLTVAGVANTDKEIAGSASGVVNMVHQIGSSVGLSI
ITALTANLISPVLSYDHAIIIMAVFMLISVGATLNIGLKK

Sequences:

>Translated_440_residues
MNKQKFGMILPIILISYFMILLDNSIVFTSTVKIAADLSLSASELSWVTNAYALTFGGLLMIGGRSGDIFGRRNVFLIGL
VIFSIGSLLVGLSTNGLMIIAMRALQGIGSAILAPTTLALLMDSYRDQMRTRAIVYYGATGGLGASIGLVVGGLIASYAS
WRWGFLLNVPVGILMIVLTLKYIPASKKVEGKLDWLGSILSVLGITALVYGINGASHPVPMVMTGILLLVLFVWQEKVSK
HPVTPLKLFKDRERSSAYIARFFFLGAMISYFFLTPQAMQQVYHFTPLMAAVGFLPETLPQFVFATLVSRLNEKFTNTQI
LIAGTVITLAGLVFAAVIGIQAGYWWSVAIPMVIIGIGQGFTLSPLTVAGVANTDKEIAGSASGVVNMVHQIGSSVGLSI
ITALTANLISPVLSYDHAIIIMAVFMLISVGATLNIGLKK
>Mature_440_residues
MNKQKFGMILPIILISYFMILLDNSIVFTSTVKIAADLSLSASELSWVTNAYALTFGGLLMIGGRSGDIFGRRNVFLIGL
VIFSIGSLLVGLSTNGLMIIAMRALQGIGSAILAPTTLALLMDSYRDQMRTRAIVYYGATGGLGASIGLVVGGLIASYAS
WRWGFLLNVPVGILMIVLTLKYIPASKKVEGKLDWLGSILSVLGITALVYGINGASHPVPMVMTGILLLVLFVWQEKVSK
HPVTPLKLFKDRERSSAYIARFFFLGAMISYFFLTPQAMQQVYHFTPLMAAVGFLPETLPQFVFATLVSRLNEKFTNTQI
LIAGTVITLAGLVFAAVIGIQAGYWWSVAIPMVIIGIGQGFTLSPLTVAGVANTDKEIAGSASGVVNMVHQIGSSVGLSI
ITALTANLISPVLSYDHAIIIMAVFMLISVGATLNIGLKK

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=413, Percent_Identity=25.181598062954, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI87081983, Length=412, Percent_Identity=27.1844660194175, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1788392, Length=403, Percent_Identity=27.7915632754342, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1788710, Length=416, Percent_Identity=26.9230769230769, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789042, Length=409, Percent_Identity=24.6943765281174, Blast_Score=107, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324954, Length=385, Percent_Identity=27.012987012987, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6323520, Length=383, Percent_Identity=26.3707571801567, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6319770, Length=228, Percent_Identity=29.8245614035088, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6325455, Length=155, Percent_Identity=25.8064516129032, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323935, Length=273, Percent_Identity=25.2747252747253, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=206, Percent_Identity=27.1844660194175, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=132, Percent_Identity=28.7878787878788, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47082; Mature: 47082

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQKFGMILPIILISYFMILLDNSIVFTSTVKIAADLSLSASELSWVTNAYALTFGGLL
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEEHEEHHCCCCCCHHHHHHHHHHHHHHHCCEE
MIGGRSGDIFGRRNVFLIGLVIFSIGSLLVGLSTNGLMIIAMRALQGIGSAILAPTTLAL
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
LMDSYRDQMRTRAIVYYGATGGLGASIGLVVGGLIASYASWRWGFLLNVPVGILMIVLTL
HHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHH
KYIPASKKVEGKLDWLGSILSVLGITALVYGINGASHPVPMVMTGILLLVLFVWQEKVSK
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
HPVTPLKLFKDRERSSAYIARFFFLGAMISYFFLTPQAMQQVYHFTPLMAAVGFLPETLP
CCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
QFVFATLVSRLNEKFTNTQILIAGTVITLAGLVFAAVIGIQAGYWWSVAIPMVIIGIGQG
HHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEECCCC
FTLSPLTVAGVANTDKEIAGSASGVVNMVHQIGSSVGLSIITALTANLISPVLSYDHAII
CEECCEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
IMAVFMLISVGATLNIGLKK
HHHHHHHHHCCCEEECCCCC
>Mature Secondary Structure
MNKQKFGMILPIILISYFMILLDNSIVFTSTVKIAADLSLSASELSWVTNAYALTFGGLL
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEEHEEHHCCCCCCHHHHHHHHHHHHHHHCCEE
MIGGRSGDIFGRRNVFLIGLVIFSIGSLLVGLSTNGLMIIAMRALQGIGSAILAPTTLAL
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
LMDSYRDQMRTRAIVYYGATGGLGASIGLVVGGLIASYASWRWGFLLNVPVGILMIVLTL
HHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHH
KYIPASKKVEGKLDWLGSILSVLGITALVYGINGASHPVPMVMTGILLLVLFVWQEKVSK
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
HPVTPLKLFKDRERSSAYIARFFFLGAMISYFFLTPQAMQQVYHFTPLMAAVGFLPETLP
CCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
QFVFATLVSRLNEKFTNTQILIAGTVITLAGLVFAAVIGIQAGYWWSVAIPMVIIGIGQG
HHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEECCCC
FTLSPLTVAGVANTDKEIAGSASGVVNMVHQIGSSVGLSIITALTANLISPVLSYDHAII
CEECCEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
IMAVFMLISVGATLNIGLKK
HHHHHHHHHCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]