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The map label for this gene is rsmE [H]

Identifier: 116492884

GI number: 116492884

Start: 1121544

End: 1122281

Strand: Reverse

Name: rsmE [H]

Synonym: PEPE_1122

Alternate gene names: 116492884

Gene position: 1122281-1121544 (Counterclockwise)

Preceding gene: 116492885

Following gene: 116492883

Centisome position: 61.25

GC content: 38.62

Gene sequence:

>738_bases
ATGCAAAGGTATTTTGTAAAACAGCCCCTTAAGATCCAAGCTGAACTTCAACTTTCGAAAGAAGATTCTCATCATCTGAT
TCGAGTAATGCGTACTGATATTGGTCAAGATGTGGAAGTTGTAGATCTAGATGGTAAAGTTTTCATTGCGGAAGTTTTGA
AGAGTGGATCAATTGCAACTTTAAGAACTAAAGAAGATATTACTAAGTCAGTTGAACTACCAGTTGATATTACGATTGCG
TGTGGAATTTCTAAGGGTGACAAAAACGAACAAATTATTAAACGCGGAACTGAAATGGGAGCTAAGCACTTTATATTTTT
TGAGTCGCGTTACTCGGTAGCTCGTTGGTCAGAACCTAAAGTGGAGAAGAAATTAAAAAGACTGCGTGAAATTGCCAAAG
GTGCGGCTGAGCAATCACATCGCCAGCTTATTCCTACGGTGGTATTTATTAAAGCGCCTGCACTATTTGAAACAACCATT
GAGCATCGAATGGTAGCTTATGAAGAATCGGCTAAACAAGATGAACATGCTAATTTTAAATCGCTTCTATTGCAACTAAA
GGCTGGAGACCAGCTTCTAGCCGTCTTCGGTCCTGAAGGTGGATTTGCAGAAGAGGAAATTCAAGAATTTACTGAGCATC
AATTTGTTTTAGCAGGTTTAGGGCCAAGAATTTTAAGAGCGGAAACAGCTCCGATGTATTTATTATCAGCTGTATCTTAT
CAATTCGAGCTTAAGTAA

Upstream 100 bases:

>100_bases
TACATTGATAGCCAATGATTTTAAAATTGAAGAAGTTCTACATTACGGTGAGTGGCGTGGCGTGATTGCTACTAATCGGA
AAGATGATTAAGGAGAAACT

Downstream 100 bases:

>100_bases
TAAGGAGAAAGACAATGAAAAAAATTAATAAATGGGGACTTTCTATTCATTGGCGATACATACTTTTCGCTTTCTTAATT
GGAATTGTCCTTCCAATAAT

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MQRYFVKQPLKIQAELQLSKEDSHHLIRVMRTDIGQDVEVVDLDGKVFIAEVLKSGSIATLRTKEDITKSVELPVDITIA
CGISKGDKNEQIIKRGTEMGAKHFIFFESRYSVARWSEPKVEKKLKRLREIAKGAAEQSHRQLIPTVVFIKAPALFETTI
EHRMVAYEESAKQDEHANFKSLLLQLKAGDQLLAVFGPEGGFAEEEIQEFTEHQFVLAGLGPRILRAETAPMYLLSAVSY
QFELK

Sequences:

>Translated_245_residues
MQRYFVKQPLKIQAELQLSKEDSHHLIRVMRTDIGQDVEVVDLDGKVFIAEVLKSGSIATLRTKEDITKSVELPVDITIA
CGISKGDKNEQIIKRGTEMGAKHFIFFESRYSVARWSEPKVEKKLKRLREIAKGAAEQSHRQLIPTVVFIKAPALFETTI
EHRMVAYEESAKQDEHANFKSLLLQLKAGDQLLAVFGPEGGFAEEEIQEFTEHQFVLAGLGPRILRAETAPMYLLSAVSY
QFELK
>Mature_245_residues
MQRYFVKQPLKIQAELQLSKEDSHHLIRVMRTDIGQDVEVVDLDGKVFIAEVLKSGSIATLRTKEDITKSVELPVDITIA
CGISKGDKNEQIIKRGTEMGAKHFIFFESRYSVARWSEPKVEKKLKRLREIAKGAAEQSHRQLIPTVVFIKAPALFETTI
EHRMVAYEESAKQDEHANFKSLLLQLKAGDQLLAVFGPEGGFAEEEIQEFTEHQFVLAGLGPRILRAETAPMYLLSAVSY
QFELK

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=244, Percent_Identity=28.6885245901639, Blast_Score=88, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27742; Mature: 27742

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRYFVKQPLKIQAELQLSKEDSHHLIRVMRTDIGQDVEVVDLDGKVFIAEVLKSGSIAT
CCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCCEEE
LRTKEDITKSVELPVDITIACGISKGDKNEQIIKRGTEMGAKHFIFFESRYSVARWSEPK
EECHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCCCHHCCCCCH
VEKKLKRLREIAKGAAEQSHRQLIPTVVFIKAPALFETTIEHRMVAYEESAKQDEHANFK
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCHH
SLLLQLKAGDQLLAVFGPEGGFAEEEIQEFTEHQFVLAGLGPRILRAETAPMYLLSAVSY
HHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCEEEEECCCCHHEECCCCHHHHHHHHCE
QFELK
EEECC
>Mature Secondary Structure
MQRYFVKQPLKIQAELQLSKEDSHHLIRVMRTDIGQDVEVVDLDGKVFIAEVLKSGSIAT
CCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCCEEE
LRTKEDITKSVELPVDITIACGISKGDKNEQIIKRGTEMGAKHFIFFESRYSVARWSEPK
EECHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCCCHHCCCCCH
VEKKLKRLREIAKGAAEQSHRQLIPTVVFIKAPALFETTIEHRMVAYEESAKQDEHANFK
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCHH
SLLLQLKAGDQLLAVFGPEGGFAEEEIQEFTEHQFVLAGLGPRILRAETAPMYLLSAVSY
HHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCEEEEECCCCHHEECCCCHHHHHHHHCE
QFELK
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9023197; 9384377 [H]